Pre‐ and Postprandial Total Serum Bile Acid Concentration Following Acute Liver Damage in Dogs

Abstract

Summary The importance of preprandial and postprandial total bile acids were investigated in dogs with liver damage induced by carbon tetrachloride (CCl4) administration. Six healthy and mature dogs were used. After base‐line clinical and biochemical examinations, hepatocellular damage was induced by oral CCI4 administration. Determinations of plasma total protein (TP), albumin (Alb), total and direct bilirubin (TBil, DBil), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) along with histologic examination of the livers 10 days following CCl4 administration were conducted to ensure that hepatic damage was in fact induced by the CCl4 administration. Twelve h fasting preprandial and 2 h postprandial serum total bile acids (PRSBA, POSBA) concentrations were also measured. The median plasma TP, Alb, TBil and DBil concentrations did not show any difference (P > 0.05) during the study. Plasma ALT activities increased significantly (P < 0.05, P < 0.01) between on the 3rd and 10th days of the experiment. The median serum values of PRSBA and POSBA were significantly different (P < 0.01) on the first day and continued to be different during the experiment. In conclusion, POSBA seemed to be more reliable than PRSBA in the diagnosis of hepatic necrosis.

Comparison of Postprandial and Ceruletide Serum Bile Acid Stimulation in Dogs

“…The sensitivity of pre-and postprandial SBA to detect hepatic dysfunction is discussed controversially; some authors have shown little difference between both parameters for most disorders, 22 whereas others have demonstrated PP SBA to be superior. 2,23,24 A recent study found baseline SBA concentrations to have a sensitivity of 92.2% for the detection of a congenital PSS and 81% for acquired shunting. 9 In our study, none of the dogs with tracheal collapse had increased baseline SBA and 2 of 11 dogs with a PSS were missed with only a fasting SBA measurement.…”