Pre‐ and post‐natal <i>Lactobacillus reuteri</i> supplementation decreases allergen responsiveness in infancy

Forsberg, Anna; Abrahamsson, Thomas; Björkstén, Bengt; Jenmalm, Maria C.

doi:10.1111/cea.12082

Cited by 53 publications

(43 citation statements)

References 41 publications

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“…The lower IL-1β, IL-6, CCL4 and CXCL8 responses to TLR2 stimulation in the probiotic treated infants were not dependent on differences in TLR2 mRNA expression in the probiotic and placebo group. Probiotic supplementation may be associated with an increased immune regulatory capacity during infancy, in line with our previous findings showing lower allergen responsiveness in the probiotic treated children [10]. …”

Section: Discussionsupporting

confidence: 88%

“…Infants supplemented with probiotics showed lower secretion of Th1/Th2 and anti-inflammatory cytokines and chemokines after allergen and mitogen stimulation as compared with infants receiving placebo [10]. These results suggest that Lactobacillus reuteri supplementation could enhance the immune regulatory capacity during infancy, possibly via innate-mediated mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Pre‐ and postnatal administration ofLactobacillus reuteridecreases TLR2 responses in infants

Forsberg

Abrahamsson

Björkstén

et al. 2014

Clinical & Translational All

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BackgroundMice models indicate that intact Toll like receptor (TLR) signaling may be essential for the allergy protective effects of diverse bacterial exposure observed in clinical trials and epidemiological studies. Probiotic supplementation with Lactobacillus reuteri from pregnancy week 36 and to the infant through the first year of life decreased the prevalence of IgE-associated eczema at two years (ClinicalTrials.gov NCT01285830). The effect of this supplementation on innate immune responses to bacterial products and the expression of associated TLRs were explored.MethodsBlood mononuclear cells were collected at birth, 6, 12 and 24 months from 61 infants and cultured with TLR2, 4 and 9 ligands. Cytokine and chemokine secretion was determined as well as TLR2, 4 and 9 mRNA expression.ResultsProbiotic supplementation was associated with decreased LTA (lipoteichoic acid) induced CCL4, CXCL8, IL-1β and IL-6 responses at 12 months and decreased CCL4 and IL-1β secretion at 24 months. TLR2 mRNA expression was not affected by probiotic treatment.ConclusionsDecreased responses to TLR2, the main receptor for LTA from Gram positive bacteria, in probiotic treated children seem to be dependent on factors downstream of TLR mRNA expression.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Pre‐ and postnatal administration ofLactobacillus reuteridecreases TLR2 responses in infants

Forsberg

Abrahamsson

Björkstén

et al. 2014

Clinical & Translational All

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“…9,10 This finding has been the rationale for the administration of probiotic bacteria for the prevention and/or treatment of allergies. [11][12][13] Probiotic lactobacilli and bifidobacteria are non-invasive and non-pathogenic Gram-positive bacteria possessing immunomodulatory properties that are strictly strain-dependent. 14 They have been documented to compete with pathogens and toxins for adherence to the intestinal epithelium and to promote intestinal epithelial cell survival, enhance barrier function and directly interact with cells of the immune system, such as dendritic cells (DCs).…”

Section: Introductionmentioning

confidence: 99%

Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization

et al. 2015

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Increasing numbers of clinical trials and animal experiments have shown that probiotic bacteria are promising tools for allergy prevention. Here, we analyzed the immunomodulatory properties of three selected lactobacillus strains and the impact of their mixture on allergic sensitization to Bet v 1 using a gnotobiotic mouse model. We showed that Lactobacillus (L.) rhamnosus LOCK0900, L. rhamnosus LOCK0908 and L. casei LOCK0919 are recognized via Toll-like receptor 2 (TLR2) and nucleotide-binding oligomerization domain-containing protein 2 (NOD2) receptors and stimulate bone marrow-derived dendritic cells to produce cytokines in species-and strain-dependent manners. Colonization of germ-free (GF) mice with a mixture of all three strains (Lmix) improved the intestinal barrier by strengthening the apical junctional complexes of enterocytes and restoring the structures of microfilaments extending into the terminal web. Mice colonized with Lmix and sensitized to the Bet v 1 allergen showed significantly lower levels of allergen-specific IgE, IgG1 and IgG2a and an elevated total IgA level in the sera and intestinal lavages as well as an increased transforming growth factor (TGF)-b level compared with the sensitized GF mice. Splenocytes and mesenteric lymph node cells from the Lmix-colonized mice showed the significant upregulation of TGF-b after in vitro stimulation with Bet v 1. Our results show that Lmix colonization improved the gut epithelial barrier and reduced allergic sensitization to Bet v 1. Furthermore, these findings were accompanied by the increased production of circulating and secretory IgA and the regulatory cytokine TGF-b. Thus, this mixture of three lactobacillus strains shows potential for use in the prevention of increased gut permeability and the onset of allergies in humans. Cellular & Molecular Immunology

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“…Probiotic treatment with Lactobacillus reuteri [75] has been shown to be associated with lower secretion of allergen induced Th2-and Th1-related cytokines during infancy, as well as with low IL-10 and Th2-associated CCL22 responses [76]. In our study, the differences were more marked for responses to the perennial and ubiquitously present [9] cat allergen than the food allergen OVA and the seasonal birch allergen.…”

Section: Lactobacillus Reuteri and Allergen Responsivenessmentioning

confidence: 46%

Lactobacillus reuteri, Infant Allergy Prevention and Childhood Immune Maturation

Forsberg¹

2016

Probiotics and Prebiotics in Human Nutrition and Health

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The increasing allergy prevalence in affluent countries may be caused by reduced microbial stimulation, resulting in an abnormal postnatal immune maturation. This chapter concerns the theories behind the use of probiotics in randomized prevention trials, and how this supplementation affects the immunity of pregnant women, the immune development in their children, and possibly preventing allergic diseases. Most studies investigating the underlying mechanisms have focused on postnatal microbial exposure. An increasing body of evidence from studies suggests that the maternal microbial environment during pregnancy can program the immune development of the child. In human allergy intervention studies, probiotic supplementation to the mother during pregnancy, as well as to her baby postnatally, may be important for preventive effects. Also, prenatal environmental exposures may alter gene expression via epigenetic mechanisms, aiming to induce physiological adaptations to the anticipated postnatal environment. The maternal microbial environment during pregnancy may program the immune development of the child.

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Pre‐ and post‐natal Lactobacillus reuteri supplementation decreases allergen responsiveness in infancy

Cited by 53 publications

References 41 publications

Pre‐ and postnatal administration ofLactobacillus reuteridecreases TLR2 responses in infants

Pre‐ and postnatal administration ofLactobacillus reuteridecreases TLR2 responses in infants

Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization

Lactobacillus reuteri, Infant Allergy Prevention and Childhood Immune Maturation

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