Pre- and Post-fertilization Barriers in Interspecific Hybridization between Evergreen Azalea Species and &lt;i&gt;Rhododendron uwaense&lt;/i&gt; H. Hara &amp; T. Yamanaka

Okamoto, Akihide; Ureshino, Kenji

doi:10.2503/hortj.mi-036

Cited by 8 publications

(6 citation statements)

References 28 publications

(30 reference statements)

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“…In studies on breeding compatibility, a histological analysis can provide key clues to determine the reason for reproductive obstacles. Previous studies have shown that incompatibility reactions are caused by prevention of pollen germination, pollen tube growth arrest, or pollen tube navigation during its growth (He et al, 2019;Kacar et al, 2015;Okamoto and Ureshino, 2015;Varotto et al, 1995). In a recent study, callus tissue that developed in the stigma and style blocked the development of the pollen tube and led to incompatibility of Passiflora species (P. alata, P. cincinnata, P. edulis, P. gibertii, P. mucronata) and changed the direction of crossings (Soares et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Pollen–Stigma Interaction between Vitis rotundifolia and Vitis vinifera

Wang

et al. 2022

J. Amer. Soc. Hort. Sci.

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Muscadine grape (Vitis rotundifolia) is highly resistant to many diseases and insects that attack european grape (Vitis vinifera). However, distant hybridization incompatibility between V. rotundifolia (female) and V. vinifera (male) impedes the utilization of V. rotundifolia in grape breeding. This study used fourth-dimension label-free protein quantitation to detect the key genes and pathways in the V. rotundifolia stigma after self-pollination (V. rotundifolia × V. rotundifolia) and cross-pollination (V. rotundifolia × V. vinifera). A histological analysis showed that pollen tube growth in the stigma of V. rotundifolia was arrested 8 hours after cross-pollination, but not after self-pollination. A proteomic analysis identified 32 differentially expressed proteins (DEPs) in the stigma of V. rotundifolia between self-pollination and cross-pollination. A heatmap analysis grouped these DEPs into four clusters. The top gene ontology terms were ATPase-coupled transmembrane transporter activity, extracellular region, DNA replication, and cellular carbohydrate biosynthetic process. A Kyoto Encyclopedia of Genes and Genomes analysis revealed that these DEPs participated in DNA replication and starch and sucrose metabolism pathways. The downregulated A5AY88, D7TJ35, D7SU26, F6HJI1, and F6GUE7 may have a role in cross incompatibility. This study revealed the cross incompatibility of grapes at histological and proteomic levels.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Pollen–Stigma Interaction between Vitis rotundifolia and Vitis vinifera

Wang

et al. 2022

J. Amer. Soc. Hort. Sci.

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“…Another well-known example is the cytoplasmic male sterility (CMS) which is a maternally inherited trait characterized by the inability of a plant to produce functional pollen (Eckardt 2006). Hybrid chlorophyll deficiency causes white-coloured cotyledons and this has been identified to occur as a result of incompatibility between the plastid genome and the nuclear genome (Ureshino et al 1999;Okamoto and Ureshino 2015).…”

Section: Hybridization For Crop Improvement In Brassicamentioning

confidence: 99%

“…Hybrid necrosis or death of young seedlings is another form of post-zygotic incompatibility which is associated with complexities in gene interaction (Potts and Dungey 2004;Okamoto and Ureshino 2015). In Arabidopsis for instance, it has been revealed that conflict between two gene variants or loci (DANGEROUS MIX 1 (DM1) and DANGEROUS MIX 2 (DM2)) may trigger defence reactions which can be detected phenotypically in hybrids as necrotic lesions on leaves and a decline in growth and fertility (Bomblies and Weigel 2007;Chae et al 2014).…”

Section: Hybridization For Crop Improvement In Brassicamentioning

confidence: 99%

Using wild relatives and related species to build climate resilience in Brassica crops

et al. 2021

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Climate change will have major impacts on crop production: not just increasing drought and heat stress, but also increasing insect and disease loads and the chance of extreme weather events and further adverse conditions. Often, wild relatives show increased tolerances to biotic and abiotic stresses, due to reduced stringency of selection for yield and yield-related traits under optimum conditions. One possible strategy to improve resilience in our modern-day crop cultivars is to utilize wild relative germplasm in breeding, and attempt to introgress genetic factors contributing to greater environmental tolerances from these wild relatives into elite crop types. However, this approach can be difficult, as it relies on factors such as ease of hybridization and genetic distance between the source and target, crossover frequencies and distributions in the hybrid, and ability to select for desirable introgressions while minimizing linkage drag. In this review, we outline the possible effects that climate change may have on crop production, introduce the Brassica crop species and their wild relatives, and provide an index of useful traits that are known to be present in each of these species that may be exploitable through interspecific hybridization-based approaches. Subsequently, we outline how introgression breeding works, what factors affect the success of this approach, and how this approach can be optimized so as to increase the chance of recovering the desired introgression lines. Our review provides a working guide to the use of wild relatives and related crop germplasm to improve biotic and abiotic resistances in Brassica crop species.

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“…Unilateral cross-incompatibility(UCI) is defined as asymmetric reproductive isolation, manifested by successful crosses in one direction and unsuccessful crosses in the other ( Muñoz-Sanz et al., 2020 ). Currently, UCI has been observed in banana shrub ( Xu et al., 2017 ), jacaranda tree ( Bittencourt, 2019 ), azalea ( Okamoto and Ureshino, 2015 ), etc. However, no studies have been reported on the cytological mechanism of interspecific asymmetric hybridization in the genus Camellia between Camellia oleifera and Camellia yuhsienensis.…”

Section: Introductionmentioning

confidence: 99%

Unilateral cross-incompatibility between Camellia oleifera and C. yuhsienensis provides new insights for hybridization in Camellia spp

Gong

Chang

et al. 2023

Front. Plant Sci.

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Camellia yuhsienensis was used to cross with Camellia oleifera to improve the resistance of oil camellia anthracnose. However, unilateral cross-incompatibility (UCI) between C. oleifera and C. yuhsienensis was found during the breeding process. Five C.oleifera cultivars and four C. uhsienensis materials were tested to confirm the UCI between C. oleifera and C. yuhsienensis. ‘Huashuo’ (HS) and ‘Youza 2’ (YZ2) were used to represent these two species to characterize the UCI, including pollen tube growth, fertilization and fruit development. The results demonstrated that UCI was prevalent between C. oleifera and C. yuhsienensis. The asynchronous flowering period was a pre-pollination barrier that limited mating between these two species under natural conditions. Interspecific pollen tubes were observed through the styles of these two plants, though the growth rates differed considerably. At 96 hours after pollination, the pollen tube of YZ2 barely entered the ovule, but remained at the base of the style and became swollen. However, the HS pollen tube entered the ovule 48 hours after pollination, double fertilization was observed, and the fruit and seeds developed commonly. Relative to compatible combinations, most unfertilized ovules in incompatible combinations failed to grow, turned brown 150 days after pollination, and the fruits were smaller than expected with uneven enlargement. Investigations on both semi-in vivo and in vitro pollen tubes gave us new idea for thought: the HS style has a stronger inhibitory effect on the interspecific pollen tubes, while calcium alleviates the inhibitory of styles but failed to prevent the appearance of abnormal pollen tube morphology. This study provides useful information on interspecific hybridization between C. oleifera and C. yuhsienensis for understanding reproductive isolation mechanisms and breeding programs in genus Camellia.

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Pre- and Post-fertilization Barriers in Interspecific Hybridization between Evergreen Azalea Species and <i>Rhododendron uwaense</i> H. Hara & T. Yamanaka

Cited by 8 publications

References 28 publications

Proteomic Analysis of Pollen–Stigma Interaction between Vitis rotundifolia and Vitis vinifera

Proteomic Analysis of Pollen–Stigma Interaction between Vitis rotundifolia and Vitis vinifera

Using wild relatives and related species to build climate resilience in Brassica crops

Unilateral cross-incompatibility between Camellia oleifera and C. yuhsienensis provides new insights for hybridization in Camellia spp

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