Abstract:Excellent pre-analytical stability is an essential precondition for reliable molecular profiling of circulating tumor DNA (ctDNA) in oncological diagnostics. Therefore, in vitro degradation of ctDNA and the additional release of contaminating genomic DNA from lysed blood cells must be prevented. Streck Cell-Free DNA blood collection tubes (cfDNA BCTs) have proposed advantages over standard K2EDTA tubes, but mainly have been tested in healthy individuals. Blood was collected from cancer patients (n = 53) suffer… Show more
“…Additionally, the mutational loads of samples stored in both tube types remained consistent across different cancer patient cohorts and concentrations. These findings support the use of cfDNA BCTs for collecting and storing clinical oncology specimens for up to 3 days, ensuring reliable ctDNA and mutation analysis [2].…”
Section: Preanalytical Considerations For Cfdna Analysissupporting
“…Additionally, the mutational loads of samples stored in both tube types remained consistent across different cancer patient cohorts and concentrations. These findings support the use of cfDNA BCTs for collecting and storing clinical oncology specimens for up to 3 days, ensuring reliable ctDNA and mutation analysis [2].…”
Section: Preanalytical Considerations For Cfdna Analysissupporting
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