PRE-1, a cis element sufficient to enhance cone- and rod- specific expression in differentiating zebrafish photoreceptors

Morrissey, Maria E; Shelton, Sara; Brockerhoff, Susan E.; Hurley, James B.; Kennedy, Breandán N.

doi:10.1186/1471-213x-11-3

Cited by 4 publications

(3 citation statements)

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“…Genes with higher number of RAX-binding sites on promoter are more likely to be actively regulated by Rx3, and exhibit larger change of gene expression in the rx3 -/- mutants (Figure 6 C, D). Rx binds to what is referred to as the PCE-1 element (photoreceptor conserved element-1), which is based on the TAAT homeodomain binding core sequence, in photoreceptor promoters to enhance gene expression [ 57 – 61 ]. The lack of a zebrafish Rx3-specific antibody hinders direct validation of these binding sites in the context of optic vesicle morphogenesis in vivo .…”

Section: Discussionmentioning

confidence: 99%

Genes and signaling networks regulated during zebrafish optic vesicle morphogenesis

et al. 2014

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BackgroundThe genetic cascades underpinning vertebrate early eye morphogenesis are poorly understood. One gene family essential for eye morphogenesis encodes the retinal homeobox (Rx) transcription factors. Mutations in the human retinal homeobox gene (RAX) can lead to gross morphological phenotypes ranging from microphthalmia to anophthalmia. Zebrafish rx3 null mutants produce a similar striking eyeless phenotype with an associated expanded forebrain. Thus, we used zebrafish rx3-/- mutants as a model to uncover an Rx3-regulated gene network during early eye morphogenesis.ResultsRx3-regulated genes were identified using whole transcriptomic sequencing (RNA-seq) of rx3-/- mutants and morphologically wild-type siblings during optic vesicle morphogenesis. A gene co-expression network was then constructed for the Rx3-regulated genes, identifying gene cross-talk during early eye development. Genes highly connected in the network are hub genes, which tend to exhibit higher expression changes between rx3-/- mutants and normal phenotype siblings. Hub genes down-regulated in rx3-/- mutants encompass homeodomain transcription factors and mediators of retinoid-signaling, both associated with eye development and known human eye disorders. In contrast, genes up-regulated in rx3-/- mutants are centered on Wnt signaling pathways, associated with brain development and disorders. The temporal expression pattern of Rx3-regulated genes was further profiled during early development from maternal stage until visual function is fully mature. Rx3-regulated genes exhibited synchronized expression patterns, and a transition of gene expression during the early segmentation stage when Rx3 was highly expressed. Furthermore, most of these deregulated genes are enriched with multiple RAX-binding motif sequences on the gene promoter.ConclusionsHere, we assembled a comprehensive model of Rx3-regulated genes during early eye morphogenesis. Rx3 promotes optic vesicle morphogenesis and represses brain development through a highly correlated and modulated network, exhibiting repression of genes mediating Wnt signaling and concomitant enhanced expression of homeodomain transcription factors and retinoid-signaling genes.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-825) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Genes and signaling networks regulated during zebrafish optic vesicle morphogenesis

et al. 2014

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“…Because expression of the transcription factor Nrl is found infrequently in ONL cells of the rod lineage (Nelson et al, 2008), and because the mouse nrl has a key role in promoting rod differentiation, it is tempting to speculate further that transient nrl expression is the last piece of the transcriptional network required for rod development in the zebrafish. The presence of nrl mRNA in zebrafish cone progenitors (Nelson et al, 2008), and the presence of Nrl response elements in an in vivo enhancer of both rod and cone photoreceptor gene expression (Morrissey et al, 2011) argue against this possibility. However, Nrl may be required in nascent cones as a transcriptional repressor (Hennig et al , 2008).…”

Section: The Rod Lineage Of Teleost Fishmentioning

confidence: 99%

The rod photoreceptor lineage of teleost fish

Stenkamp

2011

Progress in Retinal and Eye Research

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The retinas of postembryonic teleost fish continue to grow for the lifetime of the fish. New retinal cells are added continuously at the retinal margin, by stem cells residing at the circumferential germinal zone. Some of these retinal cells differentiate as Müller glia with cell bodies that reside within the inner nuclear layer. These glia retain some stem cell properties in that they carry out asymmetric cell divisions and continuously generate a population of transit-amplifying cells – the rod photoreceptor lineage – that are committed to rod photoreceptor neurogenesis. These rod progenitors progress through a stereotyped sequence of changes in gene expression as they continue to divide and migrate to the outer nuclear layer. Now referred to as rod precursors, they undergo terminal mitoses and then differentiate as rods, which are inserted into the existing array of rod and cone photoreceptors. The rod lineage displays developmental plasticity, as rod precursors can respond to the loss of rods through increased proliferation, resulting in rod replacement. The stem cells of the rod lineage, Müller glia, respond to acute damage of other retinal cell types by increasing their rate of proliferation. In addition, the Müller glia in an acutely damaged retina dedifferentiate and become multipotent, generating new, functional neurons. This review focuses on the cells of the rod lineage and includes discussions of experiments over the last 30 years that led to their identification and characterization, and the discovery of the stem cells residing at the apex of the lineage. The plasticity of cells of the rod lineage, their relationships to cone progenitors, and the applications of this information for developing future treatments for human retinal disorders will also be discussed.

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“…The GTGANTG10 motif (CCAC) in the tobacco g10 gene promoter was demonstrated to play an important role in the regulation of pollen-specific gene expression [21]. Moreover, enhancer motifs in the promoter region have been found to be essential for high levels of tissue-specific gene expression, such as hTERT and CMV elements [22], the photoreceptor regulatory element-1 [23], and the C-repeat/dehydration-responsive element [24]. Thus, detailed research on pollen-specific promoters should further our understanding of the molecular regulation mechanism of pollen development and sexual reproduction.…”

Section: Introductionmentioning

confidence: 99%

Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter

et al. 2020

IJMS

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The regulatory mechanisms of pollen development have potential value for applications in agriculture, such as better understanding plant reproductive regularity. Pollen-specific promoters are of vital importance for the ectopic expression of functional genes associated with pollen development in plants. However, there is a limited number of successful applications using pollen-specific promoters in genetic engineering for crop breeding and hybrid generation. Our previous work led to the identification and isolation of the OsSUT3 promoter from rice. In this study, to analyze the effects of different putative regulatory motifs in the OsSUT3 promoter, a series of promoter deletions were fused to a GUS reporter gene and then stably introduced into rice and Arabidopsis. Histochemical GUS analysis of transgenic plants revealed that p385 (from −385 to −1) specifically mediated maximal GUS expression in pollen tissues. The S region (from −385 to −203) was the key region for controlling the pollen-specific expression of a downstream gene. The E1 (−967 to −606), E2 (−202 to −120), and E3 (−119 to −1) regions enhanced ectopic promoter activity to different degrees. Moreover, the p385 promoter could alter the expression pattern of the 35S promoter and improve its activity when they were fused together. In summary, the p385 promoter, a short and high-activity promoter, can function to drive pollen-specific expression of transgenes in monocotyledon and dicotyledon transformation experiments.

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PRE-1, a cis element sufficient to enhance cone- and rod- specific expression in differentiating zebrafish photoreceptors

Cited by 4 publications

References 51 publications

Genes and signaling networks regulated during zebrafish optic vesicle morphogenesis

Genes and signaling networks regulated during zebrafish optic vesicle morphogenesis

The rod photoreceptor lineage of teleost fish

Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter

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