Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter

Abstract

The regulatory mechanisms of pollen development have potential value for applications in agriculture, such as better understanding plant reproductive regularity. Pollen-specific promoters are of vital importance for the ectopic expression of functional genes associated with pollen development in plants. However, there is a limited number of successful applications using pollen-specific promoters in genetic engineering for crop breeding and hybrid generation. Our previous work led to the identification and isolation of the OsSUT3 promoter from rice. In this study, to analyze the effects of different putative regulatory motifs in the OsSUT3 promoter, a series of promoter deletions were fused to a GUS reporter gene and then stably introduced into rice and Arabidopsis. Histochemical GUS analysis of transgenic plants revealed that p385 (from −385 to −1) specifically mediated maximal GUS expression in pollen tissues. The S region (from −385 to −203) was the key region for controlling the pollen-specific expression of a downstream gene. The E1 (−967 to −606), E2 (−202 to −120), and E3 (−119 to −1) regions enhanced ectopic promoter activity to different degrees. Moreover, the p385 promoter could alter the expression pattern of the 35S promoter and improve its activity when they were fused together. In summary, the p385 promoter, a short and high-activity promoter, can function to drive pollen-specific expression of transgenes in monocotyledon and dicotyledon transformation experiments.

Molecular cloning and characterization of Cinnamoyl‐CoA reductase promoter gene from Asarum sieboldii Miq

“…While analyzing the 114‐bp 5′ UTR, several motifs were found, such as the CAAT box which was located in promoter and enhancer regions, ABRE which was involved in the abscisic acid response, and G‐Box/G‐box/TCCC‐motif which was involved in response to light stress. It was reported that the 5′ UTR in the OsSUT3 promoter significantly mediated high GUS expression in pollen tissues, compared with other 5′ terminal deletion promoter fragments; and compared to CaMV35S::GUS , the GUS expression level in transgenic lines with 5′ UTR increased approximately 8.27‐fold 44 Hence, we found that the 114‐bp 5′ UTR of the AsCCR1 promoter also contained some cis ‐regulation elements that could regulate downstream gene expression, which can be of interest for future studies.…”

Isolation and Functional Characterization of a Green-Tissue Promoter in Japonica Rice (Oryza sativa subsp. Japonica)

“…Analysis together with the results of 5′ end fragment deletion showed that ACE and I-box may play a decisive role in the expression of green tissue promoter GSX7R . Previous studies have shown that the ACE element in the promoter is regulated by HY5, and the lack of HY5 will widely reduce the accumulation of other photosystem proteins except for PSII protein [ 35 ]. I-box elements mainly regulate the activity of promoters in leaves, not fruits, and can control light regulatory genes [ 36 , 37 ].…”

Spatial and Temporal Expression Characteristics of the HBB Gene Family in Six Different Pig Breeds

“…qPCR was performed on an ABI 7500 machine using the SYBR Premix Ex Taq Kit (Vazyme, Nanjing, China) and 18S ribosomal ribonucleic acid ( 18S rRNA) was used as an endogenous control gene. Relative mRNA expression levels were calculated using the Lg2 -ΔΔCt method [ 31 ]; the 2 −∆∆Ct method was reviewed in [ 32 ].…”