Prauserella shujinwangii sp. nov., from a desert environment

Abstract

A Gram-positive, spore-forming, rod-shaped actinomycete, designated XJ46 T , was isolated from Xinjiang Uyghur Autonomous Region, China and subjected to a polyphasic taxonomic analysis. Morphological and chemotaxonomic characteristics of XJ46 T were identified as being similar to those of members of the genus Prauserella. The phylogenetic tree based on 16S rRNA gene sequences showed that XJ46 T shared the highest similarity (95.9 %) with Prauserella marina MS498 T . Based on its phenotypic characteristics, chemotaxonomic analysis and 16S rRNA gene sequence analysis, strain XJ46 T is proposed to represent a novel species of the genus Prauserella, named Prauserella shujinwangii sp. nov. The type strain is XJ46 T (5CGMCC 4.7125 T 5JCM 19736 T ).Rare actinomycetes are usually regarded as the actinomycete strains with a lower isolation frequency than the members of the genus Streptomyces isolated by conventional methods (Fiedler et al., 2008; Kurtböke, 2012). The identification of rare actinomycetes of previously underrepresented genera from unexplored environments is considered to be an effective avenue for discovery of novel antibiotics (Tiwari & Gupta, 2012). The study of organisms from unique, untapped ecological niches, such as deep-sea, salt lakes and deserts, provides an efficient approach for identification of novel microbes which have the significant potential for producing unique active compounds. The most significant examples include the isolation of strains representing members of the genera Salinispora and Verrucosispora from marine sediments, producing salinosporamides and abyssomicins, respectively (Bister et al., 2004;Buck, 1982; Fenical et al., 2009;Fiedler et al., 2008;Goodfellow & Fiedler, 2010;Keller et al., 2007;Maldonado et al., 2005;Riedlinger et al., 2004;Wang et al., 2013;Williams et al., 2005). Our laboratory has been devoted to systematics-guided bioprospecting for discovery of antiinfective natural products from actinomycetes, especially the rare ones from sea sediments, salt lakes and deserts (Bian et al., 2009; Dai et al., 2010;Mao et al., 2011; Wang et al., 2010). Recently, the investigation of samples from deserts led us to isolate a novel actinomycete strain belonging to the genus Prauserella.The genus Prauserella (Kim & Goodfellow, 1999) belongs to the family Pseudonocardiaceae. This genus is described as containing aerobic, Gram-positive, non-acid-fast actinomycetes that form a well-developed substrate mycelium with irregular rod-shaped elements and aerial mycelium with spores in chains. Whole cells of the members of this genus contain meso-diaminopimelic acid in the peptidoglycan and major amounts of galactose and arabinose. The predominant menaquinone is MK-9 (H 4 ) and the DNA G+C contents are between 65.8 and 69.9 mol%. The polar lipids profiles have diphosphatidylglycerol and phosphatidylethanolamine as diagnostic components and the fattyacid profiles are rich in branched chain and saturated components (Kim & Goodfellow, 1999;Li et al., 2003). To date, this genus comprises nin...

Characterization of anti-BCG benz[α]anthraquinones and new siderophores from a Xinjiang desert–isolated rare actinomycete Nocardia sp. XJ31

“…Xinjiang area harbors lots of highly diversified ecological environments, including salt lakes, deserts, and plateaus with saline-alkali soils. Our previous efforts to isolate rare actinobacteria from extreme environments such as work in the South China Sea and a Xinjiang desert have successfully described a series of new species from genera including Verrucosispora and Prauserella (Dai et al 2010 ; Liu et al 2014b ; Wang et al 2010a , b ). In the present study, we continued a survey of the biological diversity of actinobacteria from the Xinjiang region and explored the potential anti-TB activity of NPs present in previously unknown strains.…”

Prauserella isguenensis sp. nov., a halophilic actinomycete isolated from desert soil

“…If present, the aerial mycelium forms spores in chains. At the time of writing, the genus comprised 11 recognized species, namely Prauserella rugosa (Kim & Goodfellow, 1999), P. halophila and P. alba (Li et al , 2003), P. salsuginis , P. flava , P. aidingensis and P. sediminis (Li et al , 2009), P. marina (Wang et al , 2010), P. muralis (Schäfer et al , 2010), P. shujinwangii (Liu et al , 2014) and P. coralliicola (Wu et al , 2014). The present polyphasic study was designed to determine the taxonomic position of two Prauserella -like strains.…”