Prauserella isguenensis sp. nov., a halophilic actinomycete isolated from desert soil

Abstract

Two actinomycete strains, designated H225 T and H137, were isolated from two soil samples collected from the arid region of Ahbas at Bé ni-Isguen (Mzab), located in the Algerian Sahara. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the novel strains should be assigned to the genus Prauserella of the family Pseudonocardiaceae, and they were therefore subjected to a polyphasic taxonomic study. These two strains contained mesodiaminopimelic acid as the diagnostic diamino acid and arabinose and galactose as major wholecell sugars. The diagnostic phospholipid was phosphatidylethanolamine. The predominant menaquinone was MK-9(H 4 ), and the major fatty acid was iso-C 16 : 0 . DNA-DNA hybridization values between strain H225 T and its closest phylogenetic neighbours, namely Prauserella flava DSM 45265 T , Prauserella alba DSM 44590 T , Prauserella aidingensis DSM 45266 T , Prauserella salsuginis DSM 45264 T and Prauserella sediminis DSM 45267 T , were clearly below the 70 % threshold used for species delineation. The genomic DNA G+C content of strains H225 T and H137 was 70.4 mol%. On the basis of phenotypic and genotypic data, strains H225 T and H137 T are considered to represent a novel species of the genus Prauserella, for which the name Prauserella isguenensis sp. nov. is proposed. The type strain is H225 T (5DSM 46664 T 5CECT 8577 T ).

Extreme environments: microbiology leading to specialized metabolites

“…A steady flow of filamentous actinobacteria recovered from other nonpolar deserts have been validly named as new species, they include Amycolatopsis desertii (Busarakam et al ), Desertiactinospora gelatinilytica (Saygin et al ), Nakamurella deserti (Liu et al , b), Prauserella isguensis (Saker et al ), Saccharothrix algeriensis (Zitouni et al ), Saccharothrix tharensis (Ibeyaima et al ) and Streptosporangium becharense (Chaouch et al ).…”

Modeling improved production of the chemotherapeutic polypeptide actinomycin D by a novel Streptomyces sp. strain from a Saharan soil

“…Based on inhibition zones formation, only 45 strains confirmed their higher antagonistic potential and the strain GSBNT10 revealed a potent bioactivity against both Gram positive and negative bacteria but also against MRSA (Table 7) mainly attributed to the production of antibacterial compounds. Numerous investigations confirmed the presence of diverse culturable rare and novel actinobacteria able to produce new natural products (Lamari et al., 2002; Zitouni et al., 2004; Saker et al., 2015; Chaabane-Chaouch et al., 2016).…”

Actinobacteria From Desert: Diversity and Biotechnological Applications

“…Other genera, namely, Nocardiopsis , Janibacter , Kocuria , Prauserella , and Streptosporangium , were described from the Beni Suef Governorate Desert in Egypt, the arid-saline sediment in Tunisia, the saline–alkaline desert in Egypt, the Béni Isguen Desert, and the Béni Abbès Desert in Algeria, respectively. The alkaliphilic strain of Nocardiopsis benisuefensis WS65 was isolated from the Beni Suef Governorate Desert in Egypt ( Hozzein et al, 2014 ), while a halotolerant actinobacterium Prauserella isguenensis H225 T (grew in up to 25% NaCl) was recovered from the Béni Isguen (Mzab) desert in Algeria ( Saker et al, 2015 ). Twenty-three new species belonging to nine genera ( Amycolatopsis , Blastococcus , Geodermatophilus , Lechevalieria , Lentzea , Micromonospora , Modestobacter , Pseudonocardia , and Streptomyces ) were reported and described from the Atacama Desert in Chile.…”