Pramipexole Dihydrochloride Loaded Mpegpcl Nanosuspension by Modified Nanoprecipitation: In Vitro and in Vivo Evaluation

Abstract

Objective: Nanosuspension in drug delivery is known to improve solubility, dissolution, and eventually bioavailability of the drugs in the brain. This study was carried out to show the neuroprotective effect of pramipexole dihydrochloride nanosuspension for the potential treatment in Parkinson's disease and to evaluate the effectiveness in both in vitro and in vivo models.Methods: Nanosuspension of pramipexole dihydrochloride was prepared with methoxy polyethylene glycol-poly (caprolactone) (MPEG-PCL) and Pluronic F68 by the process of modified nanoprecipitation technique with different concentrations of MPEG-PCL. The particle size, zeta potential, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and in vitro drug release were performed. The cell viability study was performed using SH-SY5Y cells. Further, the formulation is evaluated for its antioxidant potential against rotenone-induced neuronal damage in male Wister rats such as enzymatic, non-enzymatic antioxidants, and histopathological evaluation.Result: The nanoformulation shows least particle size of 143 nm and maximum zeta potential value of 33.4 mv with 88.53% entrapment efficiency were observed with PMPNP 2 formulation. The SEM, TEM, and in vitro drug release of PMPNP 2 were showed spherical shape with controlled release when compared to other formulations. Further, the MTT assay was performed using SH-SY5Y cells which show more than 50% cell viability with 50 μl of PPMNP 2 nanoformulation. Moreover, the antioxidant potential done in rotenone-induced neuronal damage in Wister rats. The results showed elevation in the levels of enzymatic and non-enzymatic antioxidants compared with the neuronal toxic group, and the nanoformulation group showed a decrease in levels of lipid peroxidation which correlates with histopathological architecture. Conclusion:This study concluded that our nanoformulation shows a significant advantage of neuroprotective effect than the plain formulation for the treatment of Parkinson's disease in both in vitro and in vivo studies.

Nanocrystal Suspension of Cefixime Trihydrate Preparation by High-Pressure Homogenization Formulation Design Using 23 Factorial Design

“…Non-ionic stabilizers are reported to sterically stabilize the nanoparticles [35]. In general, bulky and non-ionic surfactants are reported to be less toxic than single-chain and ionic surfactants [36][37][38]. Hence, PVP K-30 was selected for further investigations for the formulation.…”

Artemether Loaded Ethylcellulose Nanosuspensions: Effects of Formulation Variables, Physical Stability and Drug Release Profile

“…Many reports have focused on the characterization and qualifications of the formulated nanosuspension products [6,7] whilst others have documented the individual effects of formulation or processing variables on the attributes of nanosuspensions of different drugs [8,9]. However, the joint influences of formulation variables on particles properties of nanosuspensions have received less recognition in the relevant literature.…”