Practical quantification of necrosis in histological whole-slide images

Homeyer, André; Schenk, Andrea; Arlt, Janine; Dahmen, Uta; Dirsch, Olaf; Hahn, Horst K.

doi:10.1016/j.compmedimag.2013.05.002

Cited by 32 publications

(34 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this, we used a novel software developed by Fraunhofer MEVIS (Bremen, Germany), which enables the quick quantification of necrosis in entire tissue sections. A detailed description and evaluation of this software is given by Homeyer et al [20].…”

Section: Discussionmentioning

confidence: 99%

“…Two whole slide images were analyzed from each animal. Necrosis quantification was performed with automated image analysis software, as described by Homeyer et al [20]. Results of quantification were revised, and further pathologic alterations were revealed in a blinded fashion by two independent investigators, including a senior pathologist.…”

Section: Histologic Analysismentioning

confidence: 99%

See 1 more Smart Citation

Neural elements behind the hepatoprotection of remote perconditioning

Czigány

Turóczi

Kleiner

et al. 2015

Journal of Surgical Research

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Histologic Analysismentioning

confidence: 99%

Neural elements behind the hepatoprotection of remote perconditioning

Czigány

Turóczi

Kleiner

et al. 2015

Journal of Surgical Research

Self Cite

View full text Add to dashboard Cite

“…This intelligent software can be trained to either include or exclude elements in an image and can subsequently analyze in an identical fashion and with high accuracy, large numbers of sections. 51 After training the software, the results were concurred by 2 independent experienced evaluators in a blinded fashion. When the results were deviant from that of HistoCad, the software was trained anew.…”

Section: Quantification Of Immunostained Macrophagesmentioning

confidence: 99%

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies

Hristodorov¹,

Mladenov²,

Felbert³

et al. 2015

mAbs

View full text Add to dashboard Cite

(2015) Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies, mAbs, 7:5, 853-862, DOI: 10.1080/19420862.2015 To link to this article: https://doi.org/10. 1080/19420862.2015 (classical M1 or alternative M2), that play a differential role in immune regulation. In general, the M1 contribute to onset of inflammation, whereas the M2 orchestrate resolution and repair, whereby failure to switch from predominantly M1 to M2 reinforces a pro-inflammatory environment and chronic inflammation. Here, we show selective elimination of M1 macrophages in vitro by a range of CD64-targeted immunotoxins, including H22 (scFv)-ETA'. After re-polarization of already polarized macrophages, still only M1 polarization showed sensitivity toward CD64-directed immunotoxins. The selectivity for M1 was found linked to reduced endosomal protease activity in M1 macrophages as demonstrated by inhibition of endosomal proteases. Using the H22(scFv)-ETA' in a transgenic mouse model for chronic cutaneous inflammation, the M1 specificity was confirmed in vivo and a beneficial effect on inflammation demonstrated. Also ex vivo on skin biopsies from atopic dermatitis and diabetes type II patients with chronically-inflamed skin, a clear M1 specific effect was found. This indicates the potential relevance for human application. Our data show that targeting M1 macrophages through CD64 can be instrumental in developing novel intervention strategies for chronic inflammatory conditions.

show abstract

“…The software uses machine-learning methods in order to identify necrotic tissue by colour and texture features. A detailed description of the utilized algorithm and its application is given in Arlt et al and Homeyer et al [18, 19]. …”

Section: Methodsmentioning

confidence: 99%

Modulation of hepatic perfusion did not improve recovery from hepatic outflow obstruction

Arlt

Wei

Xie

et al. 2017

BMC Pharmacol Toxicol

View full text Add to dashboard Cite

BackgroundFocal hepatic venous outflow obstruction frequently occurs after extended liver resection and leads to a portal hypertension, arterial hypoperfusion and parenchymal necrosis.In this study, we investigated the pharmacological modulation of liver perfusion and hepatic damage in a surgical model of hepatic outflow obstruction after extended liver resection by administration of 5 different drugs in comparison to an operative intervention, splenectomy.MethodsMale inbred Lewis rats (Lew/Crl) were subjected to right median hepatic vein ligation + 70% partial hepatectomy. Treatment consisted of a splenectomy or the application of saline, carvedilol or isosorbide-5-mononitrate (ISMN) (5 mg · kg−1 respectively 7,2 mg · kg−1 per gavage 12 h−1). The splenectomy was performed during operation. The effect of the treatments on hepatic hemodynamics were measured in non-operated animals, immediately after operation (n = 4/group) and 24 h after operation (n = 5/group). Assessment of hepatic damage (liver enzymes, histology) and liver cell proliferation (BrdU-immunohistochemistry) was performed 24 h after operation. Furthermore sildenafil (10 μg · kg−1 i.p. 12h−1), terlipressin (0.05 mg · kg−1 i.v. 12 h−1) and octreotide (10 μg · kg−1 s.c. 12 h−1) were investigated regarding their effect on hepatic hemodynamics and hepatic damage 24 h after operation (n = 4/group).ResultsCarvedilol and ISMN significantly decreased the portal pressure in normal non-operated rats from 11,1 ± 1,1 mmHg (normal rats) to 8,4 ± 0,3 mmHg (carvedilol) respectively 7,4 ± 1,8 mmHg (ISMN). ISMN substantially reduced surgery-induced portal hypertension from 15,4 ± 4,4 mmHg to 9,6 ± 2,3 mmHg. Only splenectomy reduced the portal flow immediately after operation by approximately 25%. No treatment had an immediate effect on the hepatic arterial perfusion. In all treatment groups, portal flow increased by approximately 3-fold within 24 h after operation, whereas hepatic arterial flow decreased substantially. Neither treatment reduced hepatic damage as assessed 24 h after operation. The distribution of proliferating cells appeared very similar in all drug treated groups and the splenectomy group.ConclusionTransient relative reduction of portal pressure did not result in a reduction of hepatic damage. This might be explained by the development of portal hyperperfusion which was accompanied by arterial hypoperfusion.Electronic supplementary materialThe online version of this article (doi:10.1186/s40360-017-0155-4) contains supplementary material, which is available to authorized users.

show abstract

Practical quantification of necrosis in histological whole-slide images

Cited by 32 publications

References 19 publications

Neural elements behind the hepatoprotection of remote perconditioning

Neural elements behind the hepatoprotection of remote perconditioning

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies

Modulation of hepatic perfusion did not improve recovery from hepatic outflow obstruction

Contact Info

Product

Resources

About