Practical Plant Virology

Dijkstra, Jeanne; Jager, Cees P. de

doi:10.1007/978-3-642-72030-7

Cited by 75 publications

(16 citation statements)

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“…Then upper leaves were harvested after the appearance of symptoms (2 weeks), ground 1:10 ( w / v ) in 0.01 M phosphate buffer (pH 7.0), centrifuged at 6000 rpm for 5 min, and then the soluble phase (sap extract) was stored at −80 °C. Standard infectivity assays were carried out to estimate the amount of infectious virus in the sap preparation used, to ensure future reproducibility [ 77 ]. Chenopodium quinoa leaves (n = 6) were rub-inoculated with 20 µL of sap and the numbers of chlorotic foci were counted after 7–12 days.…”

Section: Methodsmentioning

confidence: 99%

Insight into the bZIP Gene Family in Solanum tuberosum: Genome and Transcriptome Analysis to Understand the Roles of Gene Diversification in Spatiotemporal Gene Expression and Function

Herath

Verchot

2020

IJMS

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The basic region-leucine zipper (bZIP) transcription factors (TFs) form homodimers and heterodimers via the coil–coil region. The bZIP dimerization network influences gene expression across plant development and in response to a range of environmental stresses. The recent release of the most comprehensive potato reference genome was used to identify 80 StbZIP genes and to characterize their gene structure, phylogenetic relationships, and gene expression profiles. The StbZIP genes have undergone 22 segmental and one tandem duplication events. Ka/Ks analysis suggested that most duplications experienced purifying selection. Amino acid sequence alignments and phylogenetic comparisons made with the Arabidopsis bZIP family were used to assign the StbZIP genes to functional groups based on the Arabidopsis orthologs. The patterns of introns and exons were conserved within the assigned functional groups which are supportive of the phylogeny and evidence of a common progenitor. Inspection of the leucine repeat heptads within the bZIP domains identified a pattern of attractive pairs favoring homodimerization, and repulsive pairs favoring heterodimerization. These patterns of attractive and repulsive heptads were similar within each functional group for Arabidopsis and S. tuberosum orthologs. High-throughput RNA-seq data indicated the most highly expressed and repressed genes that might play significant roles in tissue growth and development, abiotic stress response, and response to pathogens including Potato virus X. These data provide useful information for further functional analysis of the StbZIP gene family and their potential applications in crop improvement.

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Section: Methodsmentioning

confidence: 99%

Insight into the bZIP Gene Family in Solanum tuberosum: Genome and Transcriptome Analysis to Understand the Roles of Gene Diversification in Spatiotemporal Gene Expression and Function

Herath

Verchot

2020

IJMS

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“…Thermal inactivation point (TEP) as described by [16] is the temperature required for the complete inactivation of a virus in crude plant sap when exposed to heat. The result in Table 2 shows that at temperature between 35°C -65°C the virus was completely inactivated and the inoculated test plants did not show symptoms of infection by the virus.…”

Section: Number Of Inoculated Plantsmentioning

confidence: 99%

“…The dilution end-point (DEP) is the highest dilution of sap from a virus-infected plant which is still infectious, but is usually given as the range between the dilution and the next one at which the infectivity is lost [16]. Longevity in vitro (LIV) is the length of time after which a crude sap from a virus-infected plant loses its infectivity when kept at room temperature (28 +-2°C) [16]. Continuous keeping of the inoculum for 6 days, the infectivity of the virus reduced progressively and was completely non-infective in day 6 ( Table 4).…”

Section: Number Of Inoculated Plantsmentioning

confidence: 99%

Biological and Physico-chemical Properties of Causal Agent of Virus Disease of Coccinia barteri (Hook. f.) Keay in Calabar, Cross River State

Uyoh

Umoh

Toby

et al. 2020

IJPR

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The physico-chemical properties of the causal agent of virus disease of Coccinia barteri (Hook. f.) Keay were studied. The virus causing the disease was characterized using diagnostic tools such as host range, longevity in vitro, thermal inactivation point, dilution endpoint and aphid transmission. The virus was mechanically transmitted from the natural host (C. barteri) to the healthy test plants in the green house. In the biological properties, the virus was successfully transmitted by Aphis spiraecola (obtained from Chromolaena odorata (L.) R. M. King & H. Rob.) from infected Cucumeropsis mannii Naudin to a healthy C. mannii in a non-persistent manner and had a narrow host range limited to the family Cucurbitaceae. In the physico-chemical properties based on crude sap with an unknown virus concentration, beyond which infectivity was lost. It was readily inactivated by heating to 35 – 65°C for 10 minutes in determination of thermal inactivation point. The virus had a longevity in vitro of between 4 – 5 days beyond which it was non-infectious. Symptoms induced by the virus were leaf cupping, mottle chlorosis, blisters, stunted growth, rugosity, leaf malformation and mosaic patterns.

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“…Purified virus preparation is a prerequisite for studying the virus properties as well as for raising an antiserum against the virus. Antiserum against plant viruses is produced in a suitable warm blooded animal such as rabbits, rats, mice and chicken (Dijkstra and de Jager, 1998). This study aims to purify PVY,using differential centrifugation, precipitation and filtration process using Minisart filter, and to prepare an antiserum against this virus using albino New Zealand male rabbit.…”

Section: Introductionmentioning

confidence: 99%

Potato Virus Y (PVY) Purification and Antiserum Preparation

Nerway¹,

Kassim²

2014

IOSRJAVS

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This study included identification, purificationand antiserum preparation of potato virus Y (PVY).Positive reaction of PVY diagnosis using DAS-ELISA was characterized by yellow color. The results of spectrometrical tests indicated the good purification of the virus using differential centrifugation, precipitation and filtration techniques. The purity of PVY was 0.483 mg per gram of fresh plant tissue.A 260 /A 280 value of PVY purity was1.116. Antiserum preparation was done by immunization albino New Zealand male rabbit through four injections of purified PVY at interval of one week, intravenously and intramuscularly. The results of agar double diffusion and chloroplast agglutination tests indicated the successful preparation of the virus antiserum.

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Practical Plant Virology

Cited by 75 publications

References 0 publications

Insight into the bZIP Gene Family in Solanum tuberosum: Genome and Transcriptome Analysis to Understand the Roles of Gene Diversification in Spatiotemporal Gene Expression and Function

Insight into the bZIP Gene Family in Solanum tuberosum: Genome and Transcriptome Analysis to Understand the Roles of Gene Diversification in Spatiotemporal Gene Expression and Function

Biological and Physico-chemical Properties of Causal Agent of Virus Disease of Coccinia barteri (Hook. f.) Keay in Calabar, Cross River State

Potato Virus Y (PVY) Purification and Antiserum Preparation

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