Potato Virus Y (PVY) Purification and Antiserum Preparation

Abstract

This study included identification, purificationand antiserum preparation of potato virus Y (PVY).Positive reaction of PVY diagnosis using DAS-ELISA was characterized by yellow color. The results of spectrometrical tests indicated the good purification of the virus using differential centrifugation, precipitation and filtration techniques. The purity of PVY was 0.483 mg per gram of fresh plant tissue.A 260 /A 280 value of PVY purity was1.116. Antiserum preparation was done by immunization albino New Zealand male rabbit through four injections of purified PVY at interval of one week, intravenously and intramuscularly. The results of agar double diffusion and chloroplast agglutination tests indicated the successful preparation of the virus antiserum.