Practical pH for the IVF Laboratory

Swain, Jason E.; Wilding, Martin

doi:10.1177/205891581200300205

Cited by 4 publications

(3 citation statements)

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“…The need to specifically tailor atmospheric CO 2 to optimize culture medium pH has been recognized [ 25 ]. To ascertain that the correct pH is reached, one must choose a method to perform this check.…”

Section: Discussionmentioning

confidence: 99%

“…Although method validation is required when the BGA is being used outside its intended scope, no technical validation of the BGA for measurement of IVF-media has been published. Furthermore, it has been recognized that not all analyzers are equal and that it is prudent to validate the accuracy before use [ 25 , 26 ]. The BGA is recommended based on its accuracy [ 1 , 25 ], however, it is unclear how the accuracy is determined.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it has been recognized that not all analyzers are equal and that it is prudent to validate the accuracy before use [ 25 , 26 ]. The BGA is recommended based on its accuracy [ 1 , 25 ], however, it is unclear how the accuracy is determined. This is important, because the definition and method by which it is determined may differ between studies.…”

Section: Introductionmentioning

confidence: 99%

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Validation of the blood gas analyzer for pH measurements in IVF culture medium: Prevent suboptimal culture conditions

et al. 2018

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Measurement of pH in IVF-media using the blood gas analyzer (BGA) requires validation, because IVF-media is outside the intended scope of the BGA. To determine whether the Siemens Rapidpoint 500 BGA is suitable for pH measurements in IVF-media this study will validate the BGA and assess its accuracy. In this method comparison study, the pH of over three hundred IVF-media samples was measured with the BGA and a pH electrode (Hanna pH checker). The precision of both the BGA and the pH electrode were excellent (coefficient variation <1.4%). However, the closeness of agreement between measured values of both devices were not equivalent to each other in the tested IVF-media, showing 15% to 85% accordance between devices. The pH measured with the blood gas analyzer was also significantly higher in the tested media, compared to that measured by the pH electrode. One of the tested media did not reach its target pH when it was measured with the BGA, even at 9% CO2. The results show that the validated blood gas analyzer produces excellent results in terms of precision but not in terms of accuracy. Inaccurate measurement may lead to misinterpretation of results and consequently to suboptimal culture conditions. Therefore, each laboratory is encouraged to perform a validation of their BGA.

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Section: Discussionmentioning

confidence: 99%