Different CO2 settings (6.0% vs 7.0%) do have an impact on extracellular pH of culture medium (pHe) and euploidy rates rather than on blastocyst development: a sibling oocyte study

Abdala, Andrea; Elkhatib, Ibrahim; Bayram, A; Arnanz, Ana; El-Damen, Ahmed; Melado, Laura; Lawrenz, Barbara; Garrido, Nicolás; Fatemi, Human M.; Munck, Neelke De

doi:10.1007/s10815-021-02303-0

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…Several studies examined a possible correlation between culture settings and embryo aneuploidy. Culture of sibling oocytes under different extracellular pHs (6 vs. 7.5% CO 2 [ 69 ]; 6 vs. 7% CO 2 [ 70 ]) had no impact on blastocyst development but a significantly lower euploidy rate was found at 7 and 7.5% CO 2 . Different single-step embryo culture media reportedly did not affect the embryo euploidy of sibling oocytes [ 71 ].…”

Section: Can In Vitro Culture Increase Embryo Aneuploidy?mentioning

confidence: 99%

In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?

Gualtieri,

De Gregorio,

Candela

et al. 2024

Cells

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Preimplantation embryo culture, pivotal in assisted reproductive technology (ART), has lagged in innovation compared to embryo selection advancements. This review examines the persisting gap between in vivo and in vitro embryo development, emphasizing the need for improved culture conditions. While in humans this gap is hardly estimated, animal models, particularly bovines, reveal clear disparities in developmental competence, cryotolerance, pregnancy and live birth rates between in vitro-produced (IVP) and in vivo-derived (IVD) embryos. Molecular analyses unveil distinct differences in morphology, metabolism, and genomic stability, underscoring the need for refining culture conditions for better ART outcomes. To this end, a deeper comprehension of oviduct physiology and embryo transport is crucial for grasping embryo–maternal interactions’ mechanisms. Research on autocrine and paracrine factors, and extracellular vesicles in embryo–maternal tract interactions, elucidates vital communication networks for successful implantation and pregnancy. In vitro, confinement, and embryo density are key factors to boost embryo development. Advanced dynamic culture systems mimicking fluid mechanical stimulation in the oviduct, through vibration, tilting, and microfluidic methods, and the use of innovative softer substrates, hold promise for optimizing in vitro embryo development.

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Section: Can In Vitro Culture Increase Embryo Aneuploidy?mentioning

confidence: 99%

In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?

Gualtieri,

De Gregorio,

Candela

et al. 2024

Cells

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“…A major discussion over the years has centered around stress factors that may have a negative effect on human embryos and a potential impact on the results [11,12]. Numerous stress factors have been identi ed like oxygen concentration [13,14] and CO 2 concentration [15], to mention a few. Stress factors are linked to the generation of reactive oxygen species (ROS) which may accumulate over time during growth of embryos in-vitro [16] and are known to negatively affect IVF results [17].…”

Section: Introductionmentioning

confidence: 99%

Prospective randomized comparison of applying media with or without antioxidants during the entire IVF procedure on sibling gametes, subsequent embryo culture and single-vitrified blastocyst transfer

Mizumoto,

Nagao,

Watanabe

et al. 2023

Preprint

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Background To investigate the effect of antioxidants (AOX) on human embryo development and clinical outcome when used during all IVF procedures. Methods Prospective randomized single-centre study, where cumulus-oocyte complexes and semen samples from 127 treatment cycles were equally split between study and control (with/without AOX). The primary endpoint was Day 5/6 good quality blastocyst (GQB) rate / Metaphase-II (M-II) oocyte. Results The GQB rate between the study group and control was almost significant (P = 0.050). An age-related analysis revealed significantly higher fertilization rates in the study group for the older age groups (P < 0.05) but not in the younger. Development of Good-quality Day 3 embryos / M-II oocytes was comparable in the younger and significantly higher in the older age group (P < 0.05). Total Day 5/6 blastocyst rate and Day 5/6 GQB rate was not significantly different in the younger groups. For older patients Day 5/6 blastocyst rate and Day 5/6 GQB rate were higher in the study group (P < 0.05). Blastocyst utilization rates were comparable in younger patients and significantly different in older patients (P < 0.05). A total of 188 single vitrified blastocyst transfers were performed; 94 in each group. We noted similar implantation and ongoing pregnancy rates between in the younger age group but higher, albeit not-significant rates, for older patients in the study group (48.2% vs. 28.6%; 46.4% vs. 28.6%). Respective live birth rates were 47.4% vs 51.9% and 42.9% vs 23.8% in the younger/older age group. Conclusion Using media with AOX in all IVF procedures shows age-dependent effects. Trial regintration: Approval no: 16002

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Maîtrise des conditions environnementales en FIV

Pollet-Villard,

Doshi

2023

Infertilité

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Different CO2 settings (6.0% vs 7.0%) do have an impact on extracellular pH of culture medium (pHe) and euploidy rates rather than on blastocyst development: a sibling oocyte study

Cited by 4 publications

References 38 publications

In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?

In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?

Prospective randomized comparison of applying media with or without antioxidants during the entire IVF procedure on sibling gametes, subsequent embryo culture and single-vitrified blastocyst transfer

Maîtrise des conditions environnementales en FIV

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