Practical options for microwave digestions

Kimber, Graham M.; Kokot, Serge

doi:10.1016/0165-9936(90)87045-n

Cited by 24 publications

(5 citation statements)

References 11 publications

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“…For acid digestion, a sample (ca. 0.1 dry weight) was transferred into a Teflon 1 PFA vessel covered with a double-sealed Teflon decomposition vessel (type-P-70, SAN-AI science Co. Ltd., Nagoya, Japan) with 4 ml nitric acid and heated for 3 min with a microwave (Kimber and Kokot 1990). Digested samples were then diluted to 20 ml with ultra-pure water.…”

Section: Measurements Of the Sodium And Copper Concentrationsmentioning

confidence: 99%

Over-expression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers

Wen

Pang

Matsuda³

et al. 2007

Transgenic Res

163

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An apple spermidine synthase (SPDS) gene (MdSPDS1) was verified to encode a functional protein by the complementation of the spe3 yeast mutant, which lacks the SPDS gene. To justify our hypothesis that apple SPDS is involved in abiotic stress responses and to obtain transgenic fruit trees tolerant to abiotic stresses as well, MdSPDS1-over-expressing transgenic European pear (Pyrus communis L. 'Ballad') plants were created by Agrobacterium-mediated transformation. A total of 21 transgenic lines showing various spermidine (Spd) titers and MdSPDS1 expression levels were obtained. Selected lines were exposed to salt (150 mM NaCl), osmosis (300 mM mannitol), and heavy metal (500 microM CuSO4) stresses for evaluating their stress tolerances. Transgenic line no. 32, which was revealed to have the highest Spd accumulation and expression level of MdSPDS1, showed the strongest tolerance to these stresses. When growth increments, electrolyte leakage (EL), and values of thiobarbituric acid reactive substances (TBARS) were monitored, line no. 32 showed the lowest growth inhibition and the least increase in EL or TBARS under stress conditions. Spd titers in wild-type and transgenic lines showed diverse changes upon stresses, and these changes were not consistent with the changes in MdSPDS1 expressions. Moreover, there were no differences in the sodium concentration in the shoots between the wild type and line no. 32, whereas the copper concentration was higher in the wild type than in line no. 32. Although the mechanism(s) underlying the involvement of polyamines in stress responses is not known, these results suggest that the over-expression of the SPDS gene substantially increased the tolerance to multiple stresses by altering the polyamine titers in pear. Thus, MdSPDS1-over-expressing transgenic pear plants could be used to improve desert land and/or to repair polluted environments.

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Section: Measurements Of the Sodium And Copper Concentrationsmentioning

confidence: 99%

Over-expression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers

Wen

Pang

Matsuda³

et al. 2007

Transgenic Res

163

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“…The mineralization temperature should be controlled to avoid excessive heating of the reactors. PTFE vessels are not recommended for use above 200 °C (Kimber and Kokot, 1990). Experiments were made to assess the pressure and temperature generated during digestion in the conditions described above (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…This material was chosen because it has a softening point of about 250 °C and shows low surface adsorption of ions. To avoid the leaching of contaminating substances, we have adopted the cleanup procedure recommended by Kimber and Kokot (1990) for trace analysis: The reactors were heated at 448 W in the microwave for 10 min with 50% HNO3 and then thoroughly rinsed with deionized water. Moreover, all reactors, glassware, and crucibles were kept in 10% HNO3 and were rinsed thoroughly with distilled water before use.…”

Section: Methodsmentioning

confidence: 99%

Manganese and Zinc Analysis in Milk by Microwave Oven Digestion and Platform Graphite Furnace Atomic Absorption Spectrometry

Fuente¹,

Guevara²,

Juárez³

1995

J. Agric. Food Chem.

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“…Polytetrafluoroethylene, PTFE) are the material of choice thanks to their stability, chemical inertia and transparency to MW radiation. 6 However, sample contamination may occur because of infiltration and trapping of gases or other phases into the polymer during the digestion treatment. 7,8 The employment of highly pure quartz may be a simple strategy to overcome this problem.…”

Section: Introductionmentioning

confidence: 99%

High-throughput, Multi-batch System for the Efficient Microwave Digestion of Biological Samples

et al. 2020

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In this paper, we proposed a high-throughput microwave digestion system based on multibatch reactors (three quartz test tubes inside commercial PTFE vessels). This original configuration was validated by ICP-MS analysis of several elements in biological certified reference materials (fish tissues and plankton). The proposed system was proved to be free from contamination showing very low LODs. The improved hardware configuration is therefore highly beneficial for the detection of trace elements in microsamples from the marine food web.

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Practical options for microwave digestions

Cited by 24 publications

References 11 publications

Over-expression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers

Over-expression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers

Manganese and Zinc Analysis in Milk by Microwave Oven Digestion and Platform Graphite Furnace Atomic Absorption Spectrometry

High-throughput, Multi-batch System for the Efficient Microwave Digestion of Biological Samples

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