High-throughput, Multi-batch System for the Efficient Microwave Digestion of Biological Samples

Spanu, Davide; Butti, Laura; Boldrocchi, Ginevra; Bettinetti, Roberta; Monticelli, Damiano

doi:10.2116/analsci.19a004

Cited by 21 publications

(10 citation statements)

References 18 publications

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“…All the samples were prepared by using ultrapure water produced by a Sartorius Arium mini UV Lab Water System (Varedo, Italy) and low-density polyethylene bottles after a decontamination procedure as follows: (I) soaking in a 0.4% w / w detergent solution (Nalgene L900, Thermo Scientific, Waltham, MA, USA) for a week; (II) soaking in a HNO 3 solution (2% w / w ) for a week; (III) soaking in a second HNO 3 solution (2% w / w ) for one week [ 56 ]. Bottles were thoroughly rinsed with ultrapure water between each step and before use.…”

Section: Methodsmentioning

confidence: 99%

Optimizing Antimony Speciation Analysis via Frontal Chromatography–ICP-MS to Explore the Release of PET Additives

López,

Binda,

Roncoroni

et al. 2024

Molecules

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Antimony (Sb) contamination poses significant environmental and health concerns due to its toxic nature and widespread presence, largely from anthropogenic activities. This study addresses the urgent need for an accurate speciation analysis of Sb, particularly in water sources, emphasizing its migration from polyethylene terephthalate (PET) plastic materials. Current methodologies primarily focus on total Sb content, leaving a critical knowledge gap for its speciation. Here, we present a novel analytical approach utilizing frontal chromatography coupled with inductively coupled plasma mass spectrometry (FC-ICP-MS) for the rapid speciation analysis of Sb(III) and Sb(V) in water. Systematic optimization of the FC-ICP-MS method was achieved through multivariate data analysis, resulting in a remarkably short analysis time of 150 s with a limit of detection below 1 ng kg−1. The optimized method was then applied to characterize PET leaching, revealing a marked effect of the plastic aging and manufacturing process not only on the total amount of Sb released but also on the nature of leached Sb species. This evidence demonstrates the effectiveness of the FC-ICP-MS approach in addressing such an environmental concern, benchmarking a new standard for Sb speciation analysis in consideration of its simplicity, cost effectiveness, greenness, and broad applicability in environmental and health monitoring.

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Section: Methodsmentioning

confidence: 99%

Optimizing Antimony Speciation Analysis via Frontal Chromatography–ICP-MS to Explore the Release of PET Additives

López,

Binda,

Roncoroni

et al. 2024

Molecules

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“…For each digestion batch, 3 vessels were used for SiC samples and 2 for reference blanks. The adopted strategy initially involved the exploitation of the “Vessel-Inside-Vessel” technique [ 41 , 42 ] to avoid memory effects [ 43 ]; digestion took place in small capped containers of perfluoroalkoxy (PFA, internal volume = 5 mL) placed inside commercial PTFE vessels. The sample (mass approximately equal to 50 mg) was introduced into the PFA vessel together with the mixture of acids, while 5 mL of ultrapure H 2 O was placed outside of it (i.e., inside the PTFE vessel).…”

Section: Methodsmentioning

confidence: 99%

“…Before their use, the LDPE bottles were decontaminated with a three-step process [ 42 ]: (i) washing with ultrapure H 2 O and immersion in a 0.4% v / v Nalgene L900 detergent solution for one week; (ii) rinsing with ultrapure H 2 O and placement in a 2% wt. HNO 3 solution for one week and (iii) rinsing with ultrapure H 2 O and placement, for a week, in a second solution of 2% wt.…”

Section: Methodsmentioning

confidence: 99%

Tackling the Challenging Determination of Trace Elements in Ultrapure Silicon Carbide by LA-ICP-MS

et al. 2023

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The goal of accurately quantifying trace elements in ultrapure silicon carbide (SiC) with a purity target of 5N (99.999% purity) was addressed. The unsuitability of microwave-assisted acid digestion followed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis was proved to depend mainly on the contamination induced by memory effects of PTFE microwave vessels and by the purity levels of acids, even if highly pure ones were used in a clean environment. A new analytical protocol for the direct analysis of the solid material by laser ablation coupled with ICP-MS (LA-ICP-MS) was then exploited. Different samples were studied; the best results were obtained by embedding SiC (powders or grains) in epoxy resin. This technique has the great advantage of avoiding any source of external contamination, as grinding, pressing and sintering pretreatments are totally unnecessary. Two different laser wavelengths (266 and 193 nm) were tested, and best results were obtained with the 266 nm laser. The optimized protocol allows the determination of elements down to the sub-mg/kg level with a good accuracy level.

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“…All the adsorption experiments were performed in low-density polyethylene (LDPE) bottles thoroughly cleaned and decontaminated by a procedure involving prolonged washing with a detergent solution (4 mL/L Nalgene L900) and then with a 2% wt. HNO 3 solution (see details in [33,34]). Containers used for ICP-MS analysis were washed following the same protocol.…”

Section: Reagents Materials and Solutionsmentioning

confidence: 99%

Exploring the Adsorption of Pb on Microalgae-Derived Biochar: A Versatile Material for Environmental Remediation and Electroanalytical Applications

et al. 2022

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Biochar, a carbon material obtained by pyrolysis of biomasses, is increasingly applied in environmental remediation and sensing thanks to its functional properties, cost-effectiveness and eco-friendliness. The adsorption capacity of biochar, strictly dependent on its specific surface area, heteroatom doping and surface functional groups, is crucial for these applications. Here, biochar produced at low temperature (350 °C) from a marine microalga (Nannochloropsis sp.) is proposed as an efficient adsorbent of lead (II) ions in aqueous solution; this production strategy promotes the natural self-doping of biochar without requiring harsh conditions. The kinetics and thermodynamics of the adsorption process, as well as the effect of pH, ionic strength and dissolved organic matter on the adsorption efficiency were systematically assessed. The microalgae-derived biochar shows superior adsorption performances compared to a nutshell-derived one (used as a reference of lignocellulosic feedstocks) under all the tested conditions. The microalgae-derived biochar was finally used to decorate screen-printed carbon electrodes to improve the electroanalytical performances towards the voltammetric detection of lead (II) ions. A two-fold increase in sensitivity was obtained compared to the unmodified electrode thanks to the enhanced electron transfer and adsorption properties provided by biochar. These results highlight the potentialities of microalgae-derived biochar for environmental and sensing applications.

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High-throughput, Multi-batch System for the Efficient Microwave Digestion of Biological Samples

Cited by 21 publications

References 18 publications

Optimizing Antimony Speciation Analysis via Frontal Chromatography–ICP-MS to Explore the Release of PET Additives

Optimizing Antimony Speciation Analysis via Frontal Chromatography–ICP-MS to Explore the Release of PET Additives

Tackling the Challenging Determination of Trace Elements in Ultrapure Silicon Carbide by LA-ICP-MS

Exploring the Adsorption of Pb on Microalgae-Derived Biochar: A Versatile Material for Environmental Remediation and Electroanalytical Applications

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