PP‐22 promotes autophagy and apoptosis in the nasopharyngeal carcinoma cell line CNE‐2 by inducing endoplasmic reticulum stress, downregulating STAT3 signaling, and modulating the MAPK pathway

Tan, G.‐X.; Wang, Xin‐Ning; Tang, Yunyun; Cen, Wan‐Jing; Li, Zhenhua; Wang, Guo‐Cai; Jiang, Jianwei; Wang, Xi‐Cheng

doi:10.1002/jcp.27076

Cited by 25 publications

(15 citation statements)

References 49 publications

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“…The ER stress response exists in diverse biological systems and plays roles in the regulation of cell proliferation in many tumor cells . There is also evidence that the induction of ER stress proteins plays a role in apoptosis in various cancer cells . GRP78 functions as a molecular chaperone and is a major regulator of ER stress, as it maintains the integrity of the ER and controls the activation of unfolded protein response (UPR) signaling molecules .…”

Section: Discussionmentioning

confidence: 99%

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Norcantharidin inhibits proliferation and promotes apoptosis via c‐Met/Akt/mTOR pathway in human osteosarcoma cells

et al. 2019

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Osteosarcoma (OS) is the most common malignant bone tumor and frequently affects adolescents. Norcantharidin (NCTD), a demethylated derivative of cantharidin, has been reported to exhibit anticancer activity against various types of tumors but not human OS. The aim of the present study was to evaluate the effects of NCTD on OS cell lines (MG63 and HOS) and to explore the underlying mechanisms. In the present study, the proliferation of OS cells decreased significantly, while the apoptosis was accelerated significantly after exposure to NCTD. Meanwhile, our results also indicated that NCTD could suppress the migration and invasion, decrease the colony‐forming ability and induce S phase cell cycle arrest of OS cells in a dose‐dependent manner. Moreover, our results revealed that the anticancer effects induced by NCTD on OS cells involved autophagy, mitophagy, endoplasmic reticulum stress and c‐Met pathway. Furthermore, the results of animal experiments showed that NCTD inhibited tumor growth in a xenograft model of human OS. These results provide important new insight into the possible molecular mechanisms of NCTD and highlight its potential use as an antitumor drug for human OS.

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Section: Discussionmentioning

confidence: 99%

“…Several studies have reported the induction of endoplasmic reticulum (ER) stress during apoptosis in various tumor cells. 17 Therefore, we investigated ER stress-associated proteins. Our results indicate that NCTD significantly increased the expression of GRP78, ATF-4,…”

Section: Norcantharidin Induces Endoplasmic Reticulum Stress In Ostmentioning

confidence: 99%

Norcantharidin inhibits proliferation and promotes apoptosis via c‐Met/Akt/mTOR pathway in human osteosarcoma cells

et al. 2019

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“…A recent study reported that PDZ binding kinase, a serine–threonine kinase, accelerates cell apoptosis by activating the MAPK signaling pathway in nasopharyngeal carcinoma . PP‐22 promotes apoptotic and autophagic cell death by inducing endoplasmic reticulum stress and modulation of the p38 MAPK pathway in nasopharyngeal carcinoma CNE‐2 cells . Early reports have indicated that MAPK and PI3K pathways are targets for the development of therapeutic agents against nasopharyngeal carcinoma .…”

Section: Discussionmentioning

confidence: 99%

“…45 PP-22 promotes apoptotic and autophagic cell death by inducing endoplasmic reticulum stress and modulation of the p38 MAPK pathway in nasopharyngeal carcinoma CNE-2 cells. 46 Early reports have indicated that MAPK and PI3K pathways are targets for the development of therapeutic agents against nasopharyngeal carcinoma. 47 Recently, S100A6 has been shown to elevate cell proliferation by increasing the levels of the phosphorylated p38 pathway in human nasopharyngeal carcinoma patients.…”

Section: Discussionmentioning

confidence: 99%

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

Chuang

Tang

et al. 2019

Environmental Toxicology

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Licochalcone A is widely studied in different fields and possesses antiasthmatic, antibacterial, anti‐inflammatory, antioxidative, and anticancer properties. Its antimalignancy activity on renal, liver, lung, and oral cancer has been explored. However, limited studies have been conducted on the inhibitory effects of licochalcone A in human nasopharyngeal carcinoma cells. We determined cell viability using MTT assay. Cell cycle distribution and apoptotic cell death were measured via flow cytometry. Caspase activation and mitogen‐activated protein kinase‐related proteins in nasopharyngeal cancer cells in response to licochalcone A were identified by Western blot analysis. Results indicated that licochalcone A reduces cell viability and induces apoptosis, as evidenced by the upregulation of caspase‐8 and caspase‐9, caspase‐3 activation, and cleaved‐poly ADP‐ribose polymerase expression. Treatment with licochalcone A significantly increases ERK1/2, p38, and JNK1/2 activation. Co‐administration of a JNK inhibitor (JNK‐IN‐8) or p38 inhibitor (SB203580) abolishes the activation of caspase‐9, caspase‐8, and caspase‐3 protein expression during licochalcone A treatment. These findings indicate that licochalcone A exerts a cytostatic effect through apoptosis by targeting the JNK/p38 pathway in human nasopharyngeal carcinoma cells. Therefore, licochalcone A is a promising therapeutic agent for the treatment of human nasopharyngeal cancer cells.

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“…Hara are the two legal species according to the CP 2015 edition [10], which is the main raw material of Chinese medicine including "Yunnan Baiyao" and "Gongxuening". Studies have shown that PR has diverse pharmacological effects including antitumor [11][12][13], antimicrobial [14], anthelmintic activities [15], promoting hemostasis [16], antifungal [17], and immuno-stimulating properties [18]. The major active components in PR are spirostanol saponins including polyphyllin VII, VI, I, II, which are as the markers for quality control of PR [19].…”

Section: Introductionmentioning

confidence: 99%

A Simple and Economic Limewater-based Conversion of Spirostanol Saponins: Using Paridis Rhizoma as a case study

Yu¹,

Bu²,

Shen³

et al. 2020

Preprint

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Background Green and economic conversion of spirostanol saponins in the plant is considered as an attracting area in pharmaceutical applications. We aimed to provide a practical paradigm named limewater-based conversion of spirostanol saponins (LCSS), meanwhile, a widely-used traditional Chinese herbal medicine, Paridis Rhizoma (PR), was selected as a case study. Methods Based on single factor experiments, response surface methodology (RSM) using a Box-Behnken design (BBD) was employed to optimize processing time, limewater concentration and solvent volume to obtain a maximum total saponins yield from PR. H1299, A549 and HeLa cell lines was carried out to check pharmacological effect of Crude Paridis Rhizoma (CPR) and Processed Paridis Rhizoma (PPR), and the technology was reconfirmed by another herbal medicine, Anemarrhenae Rhizoma (AR). Results The optimal conditions were: processing time of 24 h, limewater concentration of 0.12% limewater and solvent volume of 60 mL/30 g. Under these conditions, the contents of polyphyllin VII, polyphyllin II, dioscin, gracillin, and polyphyllin I had 1.131 ± 0.448, 1.835 ± 0.479, 1.430 ± 0.550, 1.761 ± 0.571 and 1.668 ± 0.360 times increasing in four batches of PR, which was responsible for the increasing of total spirostanol saponins (TSS) in PPR. In addition, the extracts of PPR exhibited stronger antitumor potential than that of CPR on H1299, A549 and HeLa cell lines based on MTT test and cell scratch test. The efficiency of proposed LCSS was then reconfirmed by Anemarrhenae Rhizome (AR), indicating its capacity in broader application. Conclusion This study depicted a LCSS strategy that may have great potential in achieving effective and economic improvement of spirostanol saponin accumulation in herbal medicines.

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PP‐22 promotes autophagy and apoptosis in the nasopharyngeal carcinoma cell line CNE‐2 by inducing endoplasmic reticulum stress, downregulating STAT3 signaling, and modulating the MAPK pathway

Cited by 25 publications

References 49 publications

Norcantharidin inhibits proliferation and promotes apoptosis via c‐Met/Akt/mTOR pathway in human osteosarcoma cells

Norcantharidin inhibits proliferation and promotes apoptosis via c‐Met/Akt/mTOR pathway in human osteosarcoma cells

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

A Simple and Economic Limewater-based Conversion of Spirostanol Saponins: Using Paridis Rhizoma as a case study

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