POZylation: a new approach to enhance nanoparticle diffusion through mucosal barriers

Abstract

The increasing use of nanoparticles in the pharmaceutical industry is generating concomitant interest in developing nanomaterials that can rapidly penetrate into, and permeate through, biological membranes to facilitate drug delivery and improve the bioavailability of active pharmaceutical ingredients. Here, we demonstrate that the permeation of thiolated silica nanoparticles through porcine gastric mucosa can be significantly enhanced by their functionalization with either 5 kDa poly(2-ethyl-2-oxazoline) or poly(ethylene glycol). Nanoparticle diffusion was assessed using two independent techniques; Nanoparticle Tracking Analysis, and fluorescence microscopy. Our results show that poly(2-ethyl-2-oxazoline) and poly(ethylene glycol) have comparable abilities to enhance diffusion of silica nanoparticles in mucin dispersions and through the gastric mucosa. These findings provide a new strategy in the design of nanomedicines, by surface modification or nanoparticle core construction, for enhanced transmucosal drug delivery.

Synthesis and evaluation of mucoadhesive acryloyl-quaternized PDMAEMA nanogels for ocular drug delivery

“…Samples for zetapotential determination were made according to literature (0.25 mg·mL -1 of 0.005M NaCl aqueous solution) and conducted in a disposable folded capillary cell. 24 Transmission electron microscopy (TEM) was performed on a 200 kV Philips CM 20 TEM microscope. Samples were transferred onto a carbon-coated copper grid and subsequently stained with uranyl acetate.…”

DLS and zeta potential – What they are and what they are not?

“…With the launch of Nanosight® series of instruments from Malvern -the use of NTA® software has increased rapidly for determining particle size [94]. Both these techniques determine particle size from the D t (section 2.1.2.2 of this review) with Stokes-Einstein equation.…”

Synthesis of thiolated, PEGylated and POZylated silica nanoparticles and evaluation of their retention on rat intestinal mucosa in vitro

“…Previously, Khutoryanskiy et al have developed thiolated silica nanoparticles and demonstrated their mucoadhesive properties on ocular (Irmukhametova et al, 2011) and urinary bladder mucosa (Mun et al, 2016). They also demonstrated that these nanoparticles could be easily functionalised via fluorescent labelling, PEGylation and POZylation (introduction of polyethylene glycols and polyoxazolines, respectively) (Irmukhametova et al, 2011;Irmukhametova et al, 2012;Mun et al, 2014a;Mansfield et al, 2015;Mansfield et al, 2016). PEGylation of thiolated silica nanoparticles was found to reduce the retention of thiolated silica nanoparticles on the ocular (Irmukhametova et al, 2011) and urinary bladder mucosal surfaces (Mun et al, 2016).…”