DLS and zeta potential – What they are and what they are not?

Bhattacharjee, Sourav

doi:10.1016/j.jconrel.2016.06.017

Cited by 2,637 publications

(1,791 citation statements)

References 125 publications

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“…In such applications, the particle surface is in a constantly changing, dynamic state, where proteins in the body absorb onto the particle forming a corona [10]. Changes in ionic strengths and molecules present in the environment can cause changes in the composition and size of this corona, and so the hydrodynamic diameter provides a means to characterise the particle under these conditions [11]. More typical characterisation approaches such as EM imaging would have difficulty in measuring such processes due to the nature of the technique and the possibility of introducing artefacts during sample preparation.…”

Section: Np Characterisation: Different Techniques For Sizingmentioning

confidence: 99%

Characterisation of particles in solution – a perspective on light scattering and comparative technologies

Maguire

Rösslein

Wick

et al. 2018

Science and Technology of Advanced Materials

201

133

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We present here a perspective detailing the current state-of-the-art technologies for the characterisation of nanoparticles (NPs) in liquid suspension. We detail the technologies involved and assess their applications in the determination of NP size and concentration. We also investigate the parameters that can influence the results and put forward a cause and effect analysis of the principle factors influencing the measurement of NP size and concentration by NP tracking analysis and dynamic light scattering, to identify areas where uncertainties in the measurement can arise. Also included are technologies capable of characterising NPs in solution, whose measurements are not based on light scattering. It is hoped that the manuscript, with its detailed description of the methodologies involved, will assist scientists in selecting the appropriate technology for characterising their materials and enabling them to comply with regulatory agencies’ demands for accurate and reliable NP size and concentration data.

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Section: Np Characterisation: Different Techniques For Sizingmentioning

confidence: 99%

Characterisation of particles in solution – a perspective on light scattering and comparative technologies

Maguire

Rösslein

Wick

et al. 2018

Science and Technology of Advanced Materials

201

133

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“…On a practical level, the most user friendly instrument used here was the DLS. The process is mostly automated with comparatively few possible issues during measurements 19,48 . DCS and NTA are available and usable by a capable professional, however there are some pitfalls.…”

Section: Particle Size Measurementsmentioning

confidence: 99%

Using single nanoparticle tracking obtained by nanophotonic force microscopy to simultaneously characterize nanoparticle size distribution and nanoparticle–surface interactions

Hristov

Araújo

et al. 2017

Nanoscale

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Comprehensive characterization of nanomaterials for medical applications is a challenging and complex task due to the multitude of parameters which need to be taken into consideration in a broad range of conditions. Routine methods such as dynamic light scattering or nanoparticle tracking analysis provide some insight into the physicochemical properties of particle dispersions. For nanomedicine applications the information they supply can be of limited use. For this reason, there is a need for new methodologies and instruments that can provide additional data on nanoparticle properties such as their interactions with surfaces. Nanophotonic force microscopy has been shown as a viable method for measuring the force between surfaces and individual particles in the nano-size range. Here we outline a further application of this technique to measure the size of single particles and based on these measurement build the distribution of a sample. We demonstrate its efficacy by comparing the size distribution obtained with nanophotonic force microscopy to established instruments, such as dynamic light scattering and differential centrifugal sedimentation. Our results were in good agreement to those observed with all other instruments. Furthermore, we demonstrate that the methodology developed in this work can be used to study complex particle mixtures and the surface alteration of materials. For all cases studied, we were able to obtain both the size and the interaction potential of the particles with a surface in a single measurement. IntroductionNanotechnology for medical applications has been a topic of much scientific interest for several decades due to its potential to address existing challenges in patient treatment 1,2 . In particular, the use of nanoparticles (NPs) as components in the design of targeted drug delivery has been an exciting concept. The field is now facing serious challenges partly due to the reduced circulation lifetime of NPs compared to more conventional approaches [3][4][5] . One of the main obstacles is that as NPs enter into living organisms they adsorb biomolecules forming a layer, known as the biomolecular corona. The NPbiomolecular complex has been shown to have a strong correlation with the "identity" of the materials and determine their biodistribution [6][7][8] . Mechanistic details of the interaction between this complex and cell/tissue surfaces in the body remain unclear, in part due to the lack of reliable methods to measure the physicochemical properties of materials in real exposure conditions. This includes, but is not limited to, accurate size of the particle-protein complex, NP-surface interaction at different conditions and the diffusivity of NPs close to a surface. Such information combined with other advanced characterization methods to study the accessible epitopes on the biomolecular corona could help elucidate the interaction mechanisms between NPs and the cell surface. The most commonly property used to characterize a NP dispersion is its size distribution. For biological ...

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“…39 Particle size and surface charge and are the two most frequently referred factors that are responsible for the enhanced biological effects of nanoparticles including cellular uptake, toxicity and dissolution. 40 Despite giving informations about the interactions with the ocular tissues, zeta potential values also gives preliminary data for the prediciton of the physical stability of particle dispersions. 41 Guidelines classifying nanoparticle dispersions with zeta potential values of ±0-10 mV, ±10-20 mV and ±20-30 mV and >±30 mV as highly unstable, relatively stable, moderately stable and highly stable, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…41 Guidelines classifying nanoparticle dispersions with zeta potential values of ±0-10 mV, ±10-20 mV and ±20-30 mV and >±30 mV as highly unstable, relatively stable, moderately stable and highly stable, respectively. 40,42 Since the corneal and conjunctival surfaces are negatively charged, main approach for the enhancement of ocular bioavailability cationic drug delivery systems are being preferred considering possesment of enhanced bioavailability due to the electrostatic interactions between the surfaces. 24,[43][44][45][46] However anionic drug delivery systems also can enhance ocular bioavailability by the help of monocarboxylate transport which processes a proton or Na + -coupled lactate systems in epithelial cells which may enhance the ocular bioavailability of the anionic drugs in a great extent.…”

Section: Resultsmentioning

confidence: 99%

Ocular Application of Dirithromycin Incorporated Polymeric Nanoparticles: an <i>In Vitro</i> Evaluation

Başaran¹

2017

tjps

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ÖZAmaç: Özellikle gözyaşı üretimi ve kırpma refleksleri gibi gözün koruyucu mekanizmalarına bağlı olarak göze topik olarak uygulanan formülasyonların göz yüzeyinden hızla uzaklaştırılması ve korneal yüzeyden verimsiz absorbsiyonu söz konusu olmaktadır. Prekorneal tutunma süresinin ve korneal permeabilitenin arttırılması ile oküler biyoyararlanımın arttırılmasında kolloidal ilaç taşıyıcı sistemler büyük önem taşımaktadır. Bu çalışmada antibakteriyel etkili bir madde olan diritromisin yüklü Kollidon ® SR yapılı polimerik nanopartiküller şiddetli oküler bakteriyel enfeksiyonların etkin tedavisi amacı ile hazırlanmıştır. Gereç ve Yöntemler: Bu çalışmada diritromisin Kollidon ® SR yapılı polimerik nanopartiküllere püskürterek kurutma yöntemi ile yüklenmiştir. Nanopartiküllerin in vitro karakteristik özellikleri üç farklı ortamda üç ay süresince detaylı olarak incelenmiştir. Bulgular: İn vitro analiz sonuçları parçacıkların üç aylık saklama süresince karakteristik özelliklerini koruduklarını göstermiştir. Sonuç: Kollidon ® SR yapılı polimerik nanopartiküllerin şiddetli oküler bakteriyel enfeksiyonların diritromisin ile tedavisinde oldukça etkili ilaç taşıyıcı sistem adayları olduklarını göstermiştir. Anahtar kelimeler: Diritromisin, Kollidon ® SR, polimerik nanopartiküller, oküler ilaç taşıyıcı sistemler Objectives: Ocular drug delivery is a difficult challenge especially with topical intillation which results in rapid drainage and non-productive drug absorption. For the improvement of the pre-corneal retention time and enhancing the corneal permeability, colloidal drug delivery systems play an important role in enhancement of the ocular bioavailability. In this study, dirithromycin incorporated Kollidon ® SR-based polymeric nanoparticles, an antibacterial agent, were formulated for the efficient treatment of severe ocular bacterial infections. Materials and Methods: In this study, dirithromycin was incorporated into the Kollidon ® SR-based nanoparticles by spray drying method. In vitro characteristic properties were evaluated in detail during the storage period of three months at three different conditions. Results: The results of in vitro analyses revealed that characteristic properties of the particles were remained unchanged during the storage period of three months. Conclusion: Kollidon ® SR-based polymeric nanoparticles are good candidates for drug delivery systems in the treatment of severe ocular bacterial infections with dirithromycin.

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DLS and zeta potential – What they are and what they are not?

Cited by 2,637 publications

References 125 publications

Characterisation of particles in solution – a perspective on light scattering and comparative technologies

Characterisation of particles in solution – a perspective on light scattering and comparative technologies

Using single nanoparticle tracking obtained by nanophotonic force microscopy to simultaneously characterize nanoparticle size distribution and nanoparticle–surface interactions

Ocular Application of Dirithromycin Incorporated Polymeric Nanoparticles: an <i>In Vitro</i> Evaluation

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