PoxCbh, a novel CENPB‐type HTH domain protein, regulates cellulase and xylanase gene expression in Penicillium oxalicum

Abstract

To adapt to a changing natural environment, soil filamentous fungi have developed mechanisms to sense and respond to a host of environmental factors, for example, nutrients, pH, light, and temperature (Braunsdorf et al. 2016). Generally, nutrients including sugars, nitrogen, and trace elements are required for the growth and viability of soil fungi. Soil filamentous fungi such as Trichoderma, Aspergillus, and Penicillium, and the model fungus yeast, display a preference for glucose over complex disaccharides and polysaccharides. Sensing of glucose and the regulatory mechanisms have been extensively studied in the yeast Saccharomyces cerevisiae (Rodkaer & Faergeman, 2014) as well as in ascomycete fungi. These mechanisms involve G protein-coupled cAMP/

Three-Dimensional Genome Map of the Filamentous Fungus Penicillium oxalicum

“…The regulation of expression of the genes encoding the key TFs known to regulate the expression of cellulase and xylanase genes in P. oxalicum needs to be investigated for this biomass-degrading fungus, such as clrB/POX_b01969 , cxrA/POX_a00763 , cxrB/POX_f07563 , atf1/POX_b03027 , amyR/POX_f08097 , xlnR/POX_d05133 , cbh/POX_e06803 , creA/POX_e07192 , nsdD/POX_g08615 , and mbf1/POX_g08739 . Expression of these genes showed changes to various degrees at the transcriptional level in response to nonpreferred carbon sources, including Avicel, in comparison with that on glucose ( 31 , 33 – 36 ). All the investigated genes, except atf1/POX_b03027 and amyR/POX_f08097 , exhibited found cis -interactions with adjacent up- and/or down-flanking regions of each gene.…”

Arginine methyltransferases PRMT2 and PRMT3 are essential for biosynthesis of plant-polysaccharide-degrading enzymes in Penicillium oxalicum

“…Several TFs have been identified as being involved in control of PPDE biosynthesis in P. oxalicum [1], including CxrA [2]. CxrA dynamically regulates the expression of genes encoding major PPDEs, such as cbh1, eg1, bgl1 and xyn11A, as well as regulatory genes, such as clrB, cxrC and cbh [3][4][5]. The minimal DNA binding domain, CxrA Δ1-16&59-733 was found to bind the core DNA sequences 5'-ATCAGATCCTCAAAGA-3' and 5'-GCTGAGTCCTT-3' in the promoters of cbh1 and clrB, respectively [4].…”