Kinase POGSK-3β modulates fungal plant polysaccharide-degrading enzyme production and development

Zhang, Ting; Li, Han-Zhi; Li, Wentong; Tian, Di; Ning, Yuan-Ni; Liang, Xue; Tan, Jun; Zhao, Yan-Hao; Luo, Xi; Feng, Jia‐Xun; Zhao, Shuai

doi:10.1007/s00253-023-12548-7

Cited by 4 publications

(1 citation statement)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The repressor CxrC, containing an Zn(II)2Cys6 zinc nger domain, negatively regulates the expression of genes encoding major RSDEs 10 . Moreover, other speci c regulatory factors such as translational elongation factor eEF1A 11 , G protein γ subunit GNG-1 12 , protein kinases PoxMK1 13 , PoxMKK1 14 and POGSK-3β 15 , GATA-type zinc nger protein NsdD 16 and putative methyltransferase Mtr23B 17 , are known to participate in RSDE biosynthesis in P. oxalicum.…”

Section: Introductionmentioning

confidence: 99%

A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum

Zhao,

Ning,

Liang

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Filamentous fungi produce polysaccharide-degrading enzymes governing tolerance to changing environments, and this is controlled by a poorly understood transcriptional circuit. Here, genome-wide screening and genetic analyses reveal a novel regulatory circuit comprising RsrC-RsrA-RsrB that positively regulates the production of raw starch-degrading enzymes (RSDEs) in Penicillium oxalicum. Transcription factor (TF) RsrA is essential for biosynthesis of RSDEs. Two novel TFs, RsrB and RsrC, containing Zn2Cys6 and C2H2 zinc finger domains, respectively, act downstream and upstream of RsrA. RsrA activates transcription of rsrB, and three nucleotides (G-286, G-287 and G-292) are required for RsrA binding to rsrB. Polypeptide RsrB165-271 binds the conserved DNA sequence 5’-KBKWYSNRKNDVVBS-3’ in the promoters of genes encoding major amylases. RsrC specifically binds rsrA promoter via bases -850 to -825, but not the promoters of amylase genes. This regulatory circuit influences mycelial growth and conidia production. The findings expand the complex regulatory network of fungal RSDE biosynthesis and development.

show abstract