The advent of autonomous navigation, positioning, and general robotics technologies has enabled the improvement of small to miniature-sized unmanned aerial vehicles (UAVs, or ‘drones’) and their wide uses in engineering practice. Recent research endeavors further envision a systematic integration of aerial drones and traditional contact-based or ground-based sensors, leading to an aerial–ground wireless sensor network (AG-WSN), in which the UAV serves as both a gateway besides and a remote sensing platform. This paper serves two goals. First, we will review the recent development in architecture, design, and algorithms related to UAVs as a gateway and particularly illustrate its nature in realizing an opportunistic sensing network. Second, recognizing the opportunistic sensing need, we further aim to focus on achieving energy efficiency through developing an active radio frequency (RF)-based wake-up mechanism for aerial–ground data transmission. To prove the effectiveness of energy efficiency, several sensor wake-up solutions are physically implemented and evaluated. The results show that the RF-based wake-up mechanism can potentially save more than 98.4% of the energy that the traditional duty-cycle method would otherwise consume, and 96.8% if an infrared-receiver method is used.