Povidone iodine suppresses LPS-induced inflammation by inhibiting TLR4/MyD88 formation in airway epithelial cells

Abstract

Povidone-iodine (PVP-I) is an antiseptic and a disinfectant with broad-spectrum antimicrobial activity against various pathogens. However, it is unclear whether PVP-I nasal instillation can suppress mucosal inflammation in non-eosinophilic chronic rhinosinusitis (CRS) mice. This study aimed to explore the anti-inflammatory effects and underlying molecular mechanism of PVP-I on lipopolysaccharide-stimulated airway epithelial cells and investigate whether nasal instillation of PVP-I can suppress mucosal inflammation in non-eosinophilic CRS mice. Inflammation-related molecules in the nasal epithelial cells and non-eosinophilic CRS mice were measured by enzyme-linked immunosorbent assay, western blotting, quantitative real-time polymerase chain reaction, immunoprecipitation, and histopathological analysis. PVP-I blocked expressions of various inflammation-related molecules, such as NLRP3, NF-κB-p65, caspase-1, and IL-1β. Translocation of NF-κB to the nucleus, and assembly of NLRP3/ASC complexes in the nasal epithelial cells and non-eosinophilic CRS mice were also restricted. Notably, PVP-I strongly blocked the receptor co-localization of TLR4 and MyD88 in the epithelial cells of nasal mucosa. We demonstrated that PVP-I significantly attenuated inflammatory molecules and cytokines via blocking the formation of TLR4 and MyD88 complexes during LPS-induced mucosal inflammation in non-eosinophilic CRS.

Nasal instillation of povidone‐iodine ameliorates ongoing mucosal inflammation in a pre‐sensitized murine model of Der p1‐induced allergic rhinitis

“…A recent study has revealed the efficacy of nasal irrigation using PVP‐I solution in diminishing secretions among individuals suffering from chronic rhinosinusitis, although the precise mechanism remains unclear 20–22 . In this study, we demonstrated that the utilization of a safe concentration of aqueous solution of PVP‐I, determined through previous experiments, for nasal cavity washing, relieves allergic symptoms and inhibits the activation of IL‐33, which plays an important role in the development of AR 23,24 …”

“…[20][21][22] In this study, we demonstrated that the utilization of a safe concentration of aqueous solution of PVP-I, determined through previous experiments, for nasal cavity washing, relieves allergic symptoms and inhibits the activation of IL-33, which plays an important role in the development of AR. 23,24 Proteases found in aeroallergens, such as pollens, HDM, and fungi, have the ability to cleave and activate human IL-33FL. 7,25 This process impacts the development of allergic asthma and also correlates with the severity of the disease.…”

“…The concentration of PVP-I used in this experiment was the concentration that we previously confirmed toxicity for other studies. 23 To compare these properties, E64, a protease inhibitor targeting cysteine residues, was used as a control. Suzuki et al reported that the administra-tion of bromelain, an active form of cysteine protease, increased the active form of IgE and IgG1 antibody responses in an animal model stimulated with Der p1, and that nasal symptoms in mice were significantly reduced by the administration of E64.…”

“…PVP‐I was used to exploit the properties of iodine to inactivate proteases by oxidizing amino acids. The concentration of PVP‐I used in this experiment was the concentration that we previously confirmed toxicity for other studies 23 . To compare these properties, E64, a protease inhibitor targeting cysteine ​​residues, was used as a control.…”

Black Phosphorus Nanosheet-Loaded Traditional Chinese Medicine Curcumin for Acute Rhinosinusitis Therapy and Its Mechanism

“…Based on the findings of the preceding tests, 2.5 μg/mL curcumin was chosen as the intervention concentration for the following trials. In HNEpC, LPS + ATP promotes inflammation. , The CCK-8 test was used to examine changes in HNEpC cellular viability following 24 h of LPS at various doses paired with 5 mM ATP intervention. Cell viability decreased after LPS intervention at various doses, as seen in Figure b, the CCK-8 assay was used to determine the toxicity of BP-C@PEG and BP to HNEpC (Figure c), after 24 h of coculture with cells, and the materials were tested using the CCK-8 method.…”