Pouchitis is associated with mucosal imbalance between interleukin-8 and interleukin-10

Abstract

Summary:Background: Mucosal lesions of pouchitis are characterized by a neutrophil infiltrate. Interleukin (1L)-8 is the main mediator involved in neutrophil recruitment and is downregulated by IL-10. Aim: To look for an imbalance between IL-8 and IL-I 0 in patients with pouchitis. Patients/Methods: 18 patients having an ileoanal pouch for ulcerative colitis were studied. Eleven had pouchitis defined by the pouchitis disease activity index of 2 7 points and 7 had no history of pouchitis. Biopsies taken at the site of inflammation or in the normal mucosa were scored for the histologic lesions, the intensity of neutrophil infiltration, and the presence of crypt abscesses. Mucosal IL-8 and IL-10 mRNA were quantified by competitive polymerase chain reaction. Results: IL-8, IL-10, and IL-IOILL-8 mRNA were similar in patients with or without pouchitis. IL-8 mRNA levels were significantly higher in patients with a histologic score >2 (p = 0.01) and in patients with crypt abscesses (p = 0.01). IL-lO/IL-8 mRNA was significantly lower in patients having a histologic score >2 (p = 0.019), a neutrophil infiltration 2 10% (p = 0.013), and crypt abscesses (p = 0.01). Conclusion: Histologic lesions of pouchitis are associated with a mucosal imbalance between IL-8 and &lo. IL-10 could be proposed as a new treatment for pouchitis.

Leukocytapheresis for the treatment of active pouchitis: a pilot study

“…5,6 The ileal pouch mucosa of patients with pouchitis produces proinfl ammatory molecules, including tumor necrosis factor (TNF)-α, with an imbalance between proinfl ammatory and antiinfl ammatory cytokines. [21][22][23] Furthermore, patients who develop pouchitis show higher activation of signal transducer and activator of transcription (STAT)-1, a transcription factor that activates genes involved in infl ammatory and immune responses, in the pouch mucosa. 24 LCAP may reduce infl ammation via the removal of activated leukocytes that produce infl ammatory cytokines such as interleukin (IL)-1, IL-6, and TNF-α, [25][26][27] and the removal of activated platelets that can produce active oxygen.…”

Ileal reservoir with ileo-anal anastomosis: Long-term complications

“…The primary illness may also play a role in this inflammation; it most commonly occurs in patients with UC [1,5]; in this population, pouchitis occurs more commonly when there are extra-intestinal manifestations of UC [9,10]. These various mechanisms alter the lining of the reservoir and promote inflammation due to an imbalance between pro-and anti-inflammatory cytokines [11,12].…”

Partially Hydrolyzed Guar Gum Attenuates the Severity of Pouchitis in a Rat Model of Ileal J Pouch-Anal Anastomosis

“…This result may suggest that the inhibition of neutrophil accumulation by PHGG could be one of the protective factors helping to decrease pouchitis. Although MPO is a well-known enzyme, characterized by powerful pro-oxidative and pro-inflammatory properties, and recently it has been proposed as a useful diagnostic tool in acute coronary syndromes [36], there are other even more specific inflammatory markers for pouchitis such as interleukin-8 (IL-8) [37]. The correlation between IL-8 values and patient's endoscopic or histologic grades of inflammation was observed in UC and pouchitis [38].…”