Partially Hydrolyzed Guar Gum Attenuates the Severity of Pouchitis in a Rat Model of Ileal J Pouch-Anal Anastomosis

Atıla, Koray; Terzi, Cem; Canda, Aras Emre; Akhisaroglu, Serpil Tanriverdi; Avci, Hatice Sanli; Sarıoğlu, Sülen; Oktay, Gülgün; Gülay, Zeynep

doi:10.1007/s10620-008-0377-9

Cited by 13 publications

(5 citation statements)

References 35 publications

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“…The ameliorative effects of PHGG on IBD, which includes conditions such as CD (8), UC (29), and IBS (30,31), are well demonstrated in animal experimental models. In 1994, a study in nine healthy Japanese men who were administered soluble dietary fiber (7 g at each meal for 2 weeks) reported that the percentage of fecal bifidobacterial increased with fiber intake, but returned to normal when the intake was stopped (6).…”

Section: Discussionmentioning

confidence: 94%

Changes in intestinal microbiota and biochemical parameters in patients with inflammatory bowel disease and irritable bowel syndrome induced by the prolonged addition of soluble fibers to usual drug therapy

Watanabe,

Inoue,

Miyamoto

et al. 2024

J. Med. Invest.

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Objectives : Partially hydrolyzed guar gum (PHGG) is a soluble dietary fiber ; in addition to improving bowel movements, it maintains intestinal health by producing short-chain fatty acids. However, majority of clinical studies on PHGG have been concluded within a month and excluded usual drug therapy. Hence, this study aimed to determine the effects of long-term consumption of PHGG, in combination with drug therapy, on gut bacteria ratios, laboratory values for inflammatory response, and fecal characteristics. Methods and Results : The study was performed in patients with irritable bowel syndrome (IBS), Crohn's disease (CD), and ulcerative colitis (UC), by the administration of PHGG for six months while they continued their usual treatment. PHGG treatment caused significant changes in patients with IBS, including an increase in the abundance of short-chain fatty acid-producing bacteria, a significant decrease in Bacteroides abundance, and normalization of the Bristol scale of stool. In patients with UC, non-significant normalization of soft stools and decrease in fecal calprotectin were observed. Adverse events were not observed in any of the groups. Conclusion : Thus, it would be beneficial to include PHGG in the usual drug therapies of patients with IBS.

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Section: Discussionmentioning

confidence: 94%

Changes in intestinal microbiota and biochemical parameters in patients with inflammatory bowel disease and irritable bowel syndrome induced by the prolonged addition of soluble fibers to usual drug therapy

Watanabe,

Inoue,

Miyamoto

et al. 2024

J. Med. Invest.

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“…These models allow for the reconstruction of surgical procedures in humans but they are relatively rarely used due to their sizes and related costs, as well as other restrictions,. Rat models are used much more frequently [12,13], in which either idiopathic pouchitis is assessed [14] or it is chemically induced, most frequently with the use of 5% dextran sulfate sodium (DSS) [15], iodoacetamide or dinitrobenzene sulfonic acid (DNBS) [16]. The rat experimental models of pouchitis pose a vast array of surgical difficulties related to the necessity of the performance of a low anastomosis.…”

Section: Discussionmentioning

confidence: 99%

Stricture of ileo-anal J pouch anastomosis increased the intensity of pouchitis in an experimental rat model

et al. 2012

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SummaryBackgroundPouchitis appears to be the most common complication after restorative proctocolectomy.Material/MethodsIn experimental models we investigated the correlation between the width of anastomosis and the frequency of pouchitis. Twenty-three Wistar rats underwent restorative proctocolectomy under pentobarbital anesthesia. Normal width anastomosis was performed in 11 animals (Group I). In the remaining 12 animals (Group II) the diameter of anastomosis was reduced by 50%. All animals were sacrificed and the pouch mucosa was histologically (Moskowitz score) and immunohistochemically (IL-1, IL-6, IL-10, IL-12 expression) examined.ResultsMorphological assessment of pouchitis symptoms based on Moskowitz scale revealed considerably more severe inflammation (p=0.0079) in the animals from Group II than in the rats from Group I. The expressions of investigated cytokines, assessed qualitatively in histopathological examination, were higher in rats with narrow anastomosis in comparison with animals with normal anastomosis.ConclusionsThe stricture of anastomosis increases the intensity of pouchitis and stimulates the production of interleukins. It seems that anastomotic stricture plays an important role in the development of pouchitis.

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“…Pouch specimens were assessed according to the criteria described by Atila et al[16]. Erosion was evaluated as: 0, negative; 1, focal erosion; 2, erosion in many regions; or 3, extensive erosion.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus acidophilusalleviates pouchitis after ileal pouch-anal anastomosis in rats

Zhang

et al. 2017

WJG

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AIMTo assess the therapeutic potential of Lactobacillus acidophilus (LA) for the treatment of pouchitis in a rat model.METHODSSprague Dawley rats underwent proctocolectomy and ileal pouch-anal anastomosis followed by administration of dextran sulfate sodium (DSS) to induce pouchitis. Rats with pouchitis were randomly divided into three groups: no intervention (NI), normal saline (NS, 3 mL/d normal saline for 7 d), and LA (3 mL/d LA at 1× 1010 colony-forming units for 7 d). General body condition was recorded and pouch specimens were obtained for histological examination. mRNA expression levels of interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor-α were determined by RT-PCR. Zonula occludens protein 1 (ZO-1) levels were measured by immunohistochemistry.RESULTSLA reduced weight loss associated with pouchitis (P < 0.05) and improved the symptoms of pouchitis in rats. Compared with the NI and NS groups, rats in the LA group showed earlier disappearance of hematochezia (6.17 ± 0.75, 6.50 ± 0.55, 3.17 ± 0.75, P < 0.05) and higher fecal scores (2.67 ± 0.48, 2.50 ± 0.51, 4.42 ± 0.50, respectively, P < 0.05). Histological scores were also lower in the LA group compared with the other two groups (7.17 ± 0.98, 8.00 ± 0.89, 4.00 ± 0.89, respectively, P < 0.05). mRNA expression levels of IL-1β, IL-6, and tumor necrosis factor-α were significantly reduced, while IL-10 mRNA levels were significantly increased in the LA group (P < 0.05, respectively). ZO-1 protein levels were also significantly increased after administration of LA (P < 0.05).CONCLUSIONLA alleviates pouchitis induced by DSS after ileal pouch-anal anastomosis by decreasing pro-inflammatory factors and increasing anti-inflammatory factors, and restoring ZO-1 expression in the mucosa.

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Partially Hydrolyzed Guar Gum Attenuates the Severity of Pouchitis in a Rat Model of Ileal J Pouch-Anal Anastomosis

Cited by 13 publications

References 35 publications

Changes in intestinal microbiota and biochemical parameters in patients with inflammatory bowel disease and irritable bowel syndrome induced by the prolonged addition of soluble fibers to usual drug therapy

Changes in intestinal microbiota and biochemical parameters in patients with inflammatory bowel disease and irritable bowel syndrome induced by the prolonged addition of soluble fibers to usual drug therapy

Stricture of ileo-anal J pouch anastomosis increased the intensity of pouchitis in an experimental rat model

Lactobacillus acidophilusalleviates pouchitis after ileal pouch-anal anastomosis in rats

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