Potentiation of the cytostatic effect of bleomycin on L5178y mouse lymphoma cells by pepleomycin

Modulation of intracellular calcium and proliferative activity of invertebrate and vertebrate cells by ethylene

“…The viability of HeLa cells and L5178y mouse lymphoma cells [ 31 ] was determined by the MTT colorimetric assay [ 32 ]. Evaluation (at 595 nm) was performed using an ELISA plate reader (BioRad 3550) equipped with the program NCIMR IIIB.…”

Clavines as antitumor agents. 3: Cytostatic activity and structure/activity relationships of 1-alkyl agroclavines and 6-alkyl 6-noragroclavines.

“…Re-alkylation at N-6 with C2 -C7 membered straight-chain substituents led again to remarkably active compounds (12)(13)(14)(15)(16)(17). Analogous removal of the methyl group at N-6 of compound 6 however, yielding 1-pentyl-6-noragroclavine (19), caused only a moderate reduction in cytostatic activity (ED50 concentration of 6 and 19: 0.87 and 1.4 uM, respectively).…”

“…It is true that C4-alkylation at N-6 of 11 increases potency approximately 2-fold compared with 1 and more than 5-fold compared with 11, but all members of the 6-alkyl 6-nor series (12)(13)(14)(15)(16)(17) are less active than the C2-C6-alkylated N-1-substituted compounds 3-7. Furthermore, there are some fluctuations within the 6-alkyl 6-nor series; compound 15 (C5) is less active than 14 (C4), but further increase of the alkyl substituent (C6, C7) again leads to more active compounds (16,17).…”

Clavines as Antitumor Agents; 21,2,3. Natural 8-Hydroxymethyl-ergoline Type Clavines and their Derivatives

“…For the dose-response experiments 5 ml cultures were initiated by inoculation of 4 X io cells/mi and incubated at 37°C for 72 h; the controls showed a generation time of 10.5 h and reached cell con-centrations of 5.2 x iO5iml. The cell growth was determined by cell count with a Cytocomp Counter (128 channel counter; system Michaelis, Mainz/Germany) (20).…”