Modulation of intracellular calcium and proliferative activity of invertebrate and vertebrate cells by ethylene

Perovic, Sanja; Seack, Jürgen; Gamulin, Vera; Müller, Wernér E.G.; Schröder, Heinz C.

doi:10.1186/1471-2121-2-7

Cited by 15 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Signaling by lipid peroxidation products has been investigated before 41 and there are some indications that ethylene itself could have a signaling function. For instance, several cell lines (of both mouse and human origin) responded with increased Ca 2+ levels and altered gene expression after incubation with an ethylene-generating system 42 . In addition, ethylene has profound neurological effects and was widely used as an anesthetic in the 1930s, although this required very high concentrations 43 .…”

Section: Discussionmentioning

confidence: 99%

Ethylene, an early marker of systemic inflammation in humans

Paardekooper

Bogaart

Kox

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Ethylene is a major plant hormone mediating developmental processes and stress responses to stimuli such as infection. We show here that ethylene is also produced during systemic inflammation in humans and is released in exhaled breath. Traces of ethylene were detected by laser spectroscopy both in vitro in isolated blood leukocytes exposed to bacterial lipopolysaccharide (LPS) as well as in vivo following LPS administration in healthy volunteers. Exposure to LPS triggers formation of ethylene as a product of lipid peroxidation induced by the respiratory burst. In humans, ethylene was detected prior to the increase of blood levels of inflammatory cytokines and stress-related hormones. Our results highlight that ethylene release is an early and integral component of in vivo lipid peroxidation with important clinical implications as a breath biomarker of bacterial infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Ethylene, an early marker of systemic inflammation in humans

Paardekooper

Bogaart

Kox

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The intracellular Ca 2+ level of HUVEC cells was quantified using the fluorescent emission method described [ 32 ]. In short, the subconfluent 2-d’s-old cultures were loaded with 10 μM Fura-2 AM (#F0888 Sigma-Aldrich) and, after washing with phosphate-buffered saline, exposed to the polyP sample, either “Na-polyP[Ca 2+ ]” or “Ca-polyP-MP” (30 μg/mL, based on Na-polyP or the dried Ca-polyP microparticles), for up to 20 min.…”

Section: Methodsmentioning

confidence: 99%

Uptake of polyphosphate microparticles in vitro (SaOS-2 and HUVEC cells) followed by an increase of the intracellular ATP pool size

et al. 2017

View full text Add to dashboard Cite

Recently two approaches were reported that addressed a vitally important problem in regenerative medicine, i. e. the successful treatment of wounds even under diabetic conditions. Accordingly, these studies with diabetic rabbits [Sarojini et al. PLoS One 2017, 12(4):e0174899] and diabetic mice [Müller et al. Polymers 2017, 9, 300] identified a novel (potential) target for the acceleration of wound healing in diabetes. Both studies propose a raise of the intracellular metabolic energy status via exogenous administration either of ATP, encapsulated into lipid vesicles, or of polyphosphate (polyP) micro-/nanoparticles. Recently this physiological polymer, polyP, was found to release metabolic energy in form of ATP into both the extra- and also intra-cellular space. In the present work the uptake mechanism of the amorphous polyP microparticles “Ca-polyP-MP” has been described and found to be a clathrin-dependent endocytosis import, based on inhibition studies with the inhibitor trifluoperazine, which blocks the clathrin-dependent endocytosis import. The experiments had been performed with SaOS-2 cells, by studying the uptake and distribution of the electron-dense particles into the cells, and with HUVEC cells, for analysis of the intracellular accumulation of polyP, visualized by fluorescent staining of polyP. Concurrently with the uptake of particular polyP the intracellular ATP level increased as well. In contrast to “Ca-polyP-MP” the soluble polyP, administered as “Na-polyP[Ca2+]”, did not cause an increase in the intracellular Ca2+ level, suggesting a different mode of action of these two forms of polyP. Based on existing data on the effect of polyP and ATP on the induction of vascularization during wound repair, both groups (Sarojini et al. and Müller et al.) propose that the acceleration of wound repair is based on an increased metabolic energy supply directly to the regenerating wound area.

show abstract

“…Addition of the ethylene-releasing compound ethephon causes an increase in the Ca 2+ levels and cell proliferation rates of three mammalian cell lines, mouse NIH-3T3 and human HeLa and SaOS-2 cells. [104] Direct application of ethylene to SaOS-2 cells also resulted in increased Ca 2+ levels.…”

Section: Animalsmentioning

confidence: 98%

“…In this species, ethylene treatment leads to increased Ca 2+ levels, altered expression of two genes including one encoding a Ca 2+ /calmodulin protein kinase, and changes in apoptosis. [102][103][104] These studies used very high levels of ethylene (up to 2000 µL L -1 ), well above the levels of ethylene where responses in plants saturate.…”

Section: Suberites Domunculamentioning

confidence: 99%

Ethylene Receptors in Nonplant Species

2019

View full text Add to dashboard Cite

The effects of ethylene and ethylene signal transduction have been long studied in plants. It was only recently reported that a single-celled cyanobacterium, Synechocystis, contains a functional ethylene receptor. However, it remains to be determined if functional ethylene receptors are more widespread. This review summarizes what is known about responses to ethylene, ethylene receptors, and signal transduction in non-plant species. A summary is provided of information gleaned from searches of sequenced genomes and the possibility that ethylene receptors are widespread in microbes is discussed.

show abstract

Modulation of intracellular calcium and proliferative activity of invertebrate and vertebrate cells by ethylene

Cited by 15 publications

References 33 publications

Ethylene, an early marker of systemic inflammation in humans

Ethylene, an early marker of systemic inflammation in humans

Uptake of polyphosphate microparticles in vitro (SaOS-2 and HUVEC cells) followed by an increase of the intracellular ATP pool size

Ethylene Receptors in Nonplant Species

Contact Info

Product

Resources

About