Potentiation of bradykinin by a liver extract

Bradykinin-potentiating peptides from the venom of Agkistrodon halys. Isolation of five bradykinin potentiators and the amino acid sequences of two of them, potentiators B and C

“…Sulfhydryl compounds (Ferreira and Rocha e Silva, 1962; Auerswald and Doleschel, 1967;Cirstea, 1965), such as cysteine, 2,3-dimercaptopropanol (BAL), or thioglycolic acid and reduced glutathione (Edery and Grunfeld, 1969), increase the size of the contraction of isolated guinea pig ileum induced by bradykinin. e-Aminocaproic acid (Doleschel and Auerswald, 1966), a tryptic hydrolysate of denatured human plasma (Aarsen, 1968), an extract of pig liver (Tewksbury and Stahmann, 1965), and products of degradation of fibrinogen by plasmin (Buluk and Malofiejew, 1969) also have bradykinin-potentiating activity. Hamberg et al (1969) attempted to isolate bradykinin-potentiating peptides from a tryptic hydrolysate of human plasma and a plasmic hydrolysate of human fibrinogen, and found that the bradykinin-bradykinin-potentiating activity on guinea pig ileum.…”

The pharmacological properties of fibrinogen degradation products

“…Gladner, Murtaugh, Folk & Laki (1963) described the potentiation of the contractile effect of bradykinin on the isolated cornu of the rat uterus by fibrinopeptide B. The contractile effect of bradykinin on the isolated guinea-pig intestine is also potentiated by products of proteolytic degradation of some protein fractions of the plasma (Hamberg, 1966), kutapressin (Tewksbury & Stahmann, 1965), products obtained from snake poisons (Fereira, 1966), substances with free SH groups (Doleschel & Auerwald, 1966) and chymotrypsin (Edery, 1966).…”