The pharmacological properties of fibrinogen degradation products

Abstract

A method of obtaining partially purified products of plasmin degradation of fibrinogen (F.D.P.) is described. The F.D.P. passes through dialysing tubes and does not lose its activity during heating for 15 min at a temperature of 56° C. In experiments on isolated organs, F.D.P. in small doses potentiates the contractile action of bradykinin, kallidin, angiotensin, histamine, 5‐hydroxytryptamine, acetylcholine, adrenaline and noradrenaline. In high concentrations, F.D.P. induces an increase in initial tonus of the smooth muscles and simultaneously lowers the sensitivity of the smooth muscles to the action of the pharmacologically active substances mentioned. A similarity in the effect of different concentrations of KC1 and different concentrations of F.D.P. on the contractile reaction of the guinea‐pig intestine to constant doses of histamine and bradykinin and on the initial tonus of the smooth muscles is observed. A hypothesis is presented that F.D.P. in small doses potentiates the contractile action of amines and polypeptides on the smooth muscles and in larger doses increases the initial tonus of these muscles by changing the concentration of the cell electrolytes.

Vascular smooth muscle response to fibrinogen degradation products and 5‐hydroxytryptamine: possible role in cerebral vasospasm in man.

“…A similar interaction has been demonstrated between fibrin(ogen) degradation products and various spasmogens including 5-hydroxytryptamine on nonvascular preparations such as the in vitro guinea-pig ileum and rat uterus (Buluk & Malofiejew, 1969;Malofiejew 1969). …”

Hypoxia-inducible factor-1 drives annexin A2 system-mediated perivascular fibrin clearance in oxygen-induced retinopathy in mice

“…The precise mechanism by which fibrin may block neoangiogenesis is unclear. Individual degradation products of fibrin and fibrinogen have been reported to stimulate such diverse and even opposing activities as vascular contractility, 35 leukocyte activation and inhibition, 36 endothelial cell proliferation and apoptosis, 37,38 and smooth muscle cell proliferation. 39 In addition, fibrin might constitute a physical barrier to the directed migration of plasmin-deficient endothelial cells or could harbor essential growth factors that are released only on localized proteolysis by plasmin.…”

Biological Effects of Degradation Products of Collagen by Bacterial Collagenase

“…As mentioned in the introduction, peptides derived from the hydrolysis of fibrinogen, albumin and globulin are similar but less potent in their effects (Buluk & Malofiejew, 1969;Sobaniec, 1974;Wigniewski et al, 1974;. All these peptides may increase the permeability of the blood-brain barrier as well as acting directly on the nervous tissue Wigniewski et al, 1975;Zwolifiski & Buczko, 1977).…”

“…Besides kinins, other peptides cleaved from blood plasma proteins increase the permeability of capillary vessels and induce changes in the arterial pressure, blood flow and heart rate (Buluk & Malofiejew, 1969;Sobaniec, 1974;Wisniewski, Tarasiewicz, Mackowiak, Buczko & Moniuszko-Jakoniuk, 1974;Buczko, Franco, Bianchetti, Donati, de Gaetano & Garattini, 1976;Buczko, 1977).…”