Potentiation of antibiotic activity of aminoglycosides by natural products from Cordia verbenacea DC

Matias, Edinardo Fágner Ferreira; Alves, Erivania Ferreira; Silva, Maria K.N.; Carvalho, Victoria R.A.; Medeiros, Cássio Rocha; Santos, Francisco de Assis Ribeiro dos; Bitu, Vanessa C.N.; Souza, Celestina Elba Sobral de; Figueredo, Fernando Gomes; Boligon, Aline Augusti; Athayde, Margareth L.; Costa, José G.M.; Coutinho, Henrique Douglas Melo

doi:10.1016/j.micpath.2016.03.009

Cited by 18 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several natural products have been investigated as to their modulatory properties, many of which present relevant synergistic effects, constituting an option in the fight against the phenomenon of microbial resistance . ‘Antibiotic activity modifiers’ is a term used to denote substances that modulate or even revert the bacterial resistance phenotype to certain antibiotics and may alter microbial susceptibility to antibiotics by inhibiting efflux pumps .…”

Section: Resultsmentioning

confidence: 99%

Antibiotic‐Potentiating Activity of Phanostenine Isolated from Cissampelos sympodialisEichler

Cunha

Camilo

Nonato

et al. 2019

Chemistry & Biodiversity

View full text Add to dashboard Cite

Cissampelos sympodialis EICHLER is well studied and investigated for its antiasthmatic properties, but there are no data in the literature describing antibacterial properties of alkaloids isolated from this botanical species. This work reports the isolation and characterization of phanostenine obtained from roots of C. sympodialis and describes for the first time its antimicrobial and antibiotic modulatory properties. Phanostenine was first isolated from Cissampelos sympodialis and its antibacterial activities were determined. Chemical structures of the alkaloid isolate were determined using spectroscopic and chemical analyses. Phanostenine was also tested for its antibacterial activity against standard strains and clinical isolates of Escherichia coli and Staphylococcus aureus. Minimal inhibitory concentration (MIC) was determined in a microdilution assay and for the evaluation of antibiotic resistance-modifying activity. MIC of the antibiotics was determined in the presence or absence of phanostenine at sub-inhibitory concentrations. The evaluation of antibacterial activity by microdilution assay showed activity for all strains with better values against S. aureus ATCC 12692 and E. coli 27 (787.69 mM). The evaluation of aminoglycoside antibiotic resistance-modifying activity showed reduction in the MIC of the aminoglycosides (amikacin, gentamicin and neomycin) when associated with phanostenine, MIC reduction of antibiotics ranging from 21 % to 80 %. The data demonstrated that phanostenine possesses a relevant ability to modify the antibiotic activity in vitro. We can suggest that phanostenine presents itself as a promising tool as an adjuvant for novel antibiotics formulations against bacterial resistance.

show abstract

Section: Resultsmentioning

confidence: 99%

Antibiotic‐Potentiating Activity of Phanostenine Isolated from Cissampelos sympodialisEichler

Cunha

Camilo

Nonato

et al. 2019

Chemistry & Biodiversity

View full text Add to dashboard Cite

show abstract

“…Some ethanolic leaf extracts of some species from this genus present biological activities, such as Cordia verbenacea and Cordia curassavica with anti-inflammatory and analgesic activity (Lameira et al, 1997;Bayeux et al, 2002), Cordia boissieri and Cordia sinensis Lam. with antioxidant activity (Al-Musayeib et al, 2011;Salazar-Aranda et al, 2011), and Cordia dichotoma and Cordia verbenacea with antimicrobial and antifungal activity (Matias et al, 2016;Pinho et al, 2012;Rahman and Akhtar 2016).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical screening of Cordia glabrata (MART.) A.DC. extracts and its potential antioxidant, photoprotective, antimicrobial and antiviral activities

et al. 2023

View full text Add to dashboard Cite

Species of the genus Cordia have shown biological activities, such as anti-inflammatory, analgesic, antioxidant, antiviral, and antifungal activities. The species Cordia glabrata (MART) A.DC. Has no information concerning its phytochemical profile and possible biological activities. Thus, this study aimed to evaluate this profile in ethanolic extracts of young, adult and senescent leaves, as well as their antioxidant, photoprotective, antimicrobial, and virucidal potentials. Phytochemical analysis was performed by TLC (thin-layer chromatography) and showed the presence of flavonoids, tannins, and terpenes. The evaluation by UPLC-MS/MS (Ultra performance liquid chromatography - tandem mass spectrometer) evidenced the presence of caffeic (3.89 mgL-1), p-cumaric (6.13 mgL-1), and ferulic (0.58 mgL-1) acids, whilst, in GC/MS (Gas chromatography–mass spectrometry) analysis there was a greater amount of palmitic (51.17%), stearic (20.34%), linoleic (9.62%), and miristic (8.16%) fatty acids. The DPPH (2,2-Diphenyl-1-picrylhydrazyl) and ABTS+ (2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radicals were used to verify the potential antioxidant activity, observing a better activity for the leaf extract in the adult phenological stage: 54.63 ± 1.06 µgmL-1 (DPPH) and 44.21 ± 1.69 mM (ABTS). The potential photoprotective activity of the extracts was determined by spectrophotometry and the in vitro values of SPF (Sun Protection Factor) in young and adult leaves (5.47 and 5.41, respectively) showed values close to the minimum SPF of 6.0 required by ANVISA (Brazilian Health Regulatory Agency). It was not observed an antimicrobial activity for Staphylococcus aureus with a minimum inhibitory concentration of 2000 μgmL-1, however the anti-herpetic assay against the Herpes simplex virus type 2 (HSV-2) showed a potent virucidal activity at the tested concentrations with CV50 value <0.195 μgmL-1 and a Selectivity Index (SI = CC50 / CV50) greater than 448. The results obtained in this study suggest that extracts of leaves of C. glabrata in their adult phenological stage have potential antioxidant, photoprotective and virucidal activity, considering in vitro test results.

show abstract

“…The substance α-humulene is considered a chemical marker in the pharmaceutical industry for the quality control of V. curassavica essential oil. Besides its anti-inflammatory activity, the species has been demonstrated to have other medicinal properties, such as anti-allergenic, antiulcerogenic, antioxidant, antitumor [5,[7][8][9][10][11], antibacterial, and antifungal [12,13].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Varronia curassavica Jacq. Terpenoids by Flow Modulated Two-Dimensional Gas Chromatography Coupled to Mass Spectrometry

2020

View full text Add to dashboard Cite

In this study, a metabolomic approach was used to investigate the effect of seasonality on the chemical composition and yield of anti-inflammatory active principle, α-humulene, in the essential oil of three genotypes of Varronia curassavica Jacq. (Syn. Cordia verbenaceae). The essential oils were extracted by hydrodistillation and analyzed by comprehensive two-dimensional gas chromatography coupled to mass spectrometry (GC×GC-MS). The GC×GC approach a three-fold improvement in qualitative analysis (48 compounds were identified by GC-MS versus 135 by GC×GC-MS). The improved resolving power of GC×GC resolved important coelutions and enabled the detection of unusual substances in V. curassavica essential oil. The chromatographic data was analyzed by using peak table-based chemometrics, namely, principal component analysis (PCA) and hierarchical cluster analysis (HCA). The metabolic study showed that seasonality has a significant effect on the chemical composition. The α-humulene content was affected by genotype and season. Spring and summer were the best harvest seasons for the yield of the active ingredient, found in higher concentrations in the VC2 genotype. The proposed metabolomic workflow was successfully applied to terpene analysis found in V. curassavica essential oil, and such results have broadened our understanding of the influence of seasonal factors on the specialized metabolism of the species.

show abstract

Potentiation of antibiotic activity of aminoglycosides by natural products from Cordia verbenacea DC

Cited by 18 publications

References 19 publications

Antibiotic‐Potentiating Activity of Phanostenine Isolated from Cissampelos sympodialisEichler

Antibiotic‐Potentiating Activity of Phanostenine Isolated from Cissampelos sympodialisEichler

Phytochemical screening of Cordia glabrata (MART.) A.DC. extracts and its potential antioxidant, photoprotective, antimicrobial and antiviral activities

Metabolic Profiling of Varronia curassavica Jacq. Terpenoids by Flow Modulated Two-Dimensional Gas Chromatography Coupled to Mass Spectrometry

Contact Info

Product

Resources

About