Potentiating Effect of .BETA.-Glucans on Photodynamic Therapy of Implanted Cancer Cells in Mice

Akramienė, Dalia; Aleksandraviciene, Ceslava; Grazeliene, G.; Žalinkevičius, Rimantas; Suziedelis, Kestutis; Didžiapetrienė, Janina; Simonsen, Ulf; Stankevičius, Edgaras; Kėvelaitis, Egidijus

doi:10.1620/tjem.220.299

Cited by 15 publications

(9 citation statements)

References 41 publications

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“…In worst case this may result in secondary malignancies after PDT. Our data are in accordance with those in murine gliobastoma cells where mTHPC-PDT also resulted in an immediate DNA damage, however, in this study and others activation of repair mechanisms after PDT had been reported [29][30][31][32].…”

Section: Discussionsupporting

confidence: 93%

Cellular and molecular effects of the liposomal mTHPC derivative Foslipos in prostate carcinoma cells in vitro

Gyenge

Hiestand

Graefe

et al. 2011

Photodiagnosis and Photodynamic Therapy

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BACKGROUND: Meso-tetra-hydroxyphenyl-chlorine (mTHPC) is among the most powerful photosensitizers available for photodynamic therapy (PDT). However, the mechanisms leading to cell death are poorly understood. We here focused on changes at DNA and RNA levels after treatment with the liposomal mTHPC derivative Foslipos in vitro. METHODS: After determination of darktoxicity, laser conditions and uptake kinetics, PC-3 prostate carcinoma cells were subjected to PDT with Foslipos, followed by assessment of cell numbers directly (TP0) or 1h (TP1), 2h (TP2), 5h (TP5) and 24h (TP24) after illumination. Nucleic acids had been extracted for evaluation of RNA amounts and integrity as well as for estimation of abasic sites as a measure for DNA damage. Furthermore, expression changes of 84 genes related to oxidative stress were investigated by quantitative polymerase chain reaction. RESULTS: Already at TP0, the number of dead cells was significantly higher after PDT versus controls and at TP24 more than 90% of cells had been destroyed. PDT resulted in a severe damage of both RNA and DNA. Gene expression analyses revealed an impact of PDT on pathways for oxidative and metabolic stress, heat shock, proliferation and carcinogenesis, growth arrest, inflammation, DNA repair and apoptosis signaling. CONCLUSIONS: Mechanisms of Foslipos-mediated PDT comprise a combination of acute and delayed lethal effects in PC-3 cells. The latter may include death processes initiated by nucleic acid damage, activation of stress and growth arrest genes in combination with a reduced capability to adequately cope with oxidative toxicity. Our results will help to better understand molecular photodynamic effects. AbstractBackground: Meso-tetra-hydroxyphenyl-chlorine (mTHPC) is among the most powerful

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Section: Discussionsupporting

confidence: 93%

Cellular and molecular effects of the liposomal mTHPC derivative Foslipos in prostate carcinoma cells in vitro

Gyenge

Hiestand

Graefe

et al. 2011

Photodiagnosis and Photodynamic Therapy

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“…Polysaccharides were fermented into propionic and butyric acids by intestinal microbiota, similar to the effects of prebiotics. These results suggest that the fermentation of laminaran by intestinal bacteria could suppress the risk of colorectal cancer [87,88]. It is of special interest that not only laminarans, but also other β- d -glucans, isolated from yeast, fungi and cereals demonstrated anti-cytotoxic, anti-mutagenic, and anti-tumorigenic properties, making this class of polysaccharides a promising promoter of health [89].…”

Section: Polysaccharides From Brown Algaementioning

confidence: 99%

Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects

et al. 2013

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Many marine-derived polysaccharides and their analogues have been reported as showing anticancer and cancer preventive properties. These compounds demonstrate interesting activities and special modes of action, differing from each other in both structure and toxicity profile. Herein, literature data concerning anticancer and cancer preventive marine polysaccharides are reviewed. The structural diversity, the biological activities, and the molecular mechanisms of their action are discussed.

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“…The cytotoxicity studies of coladerm membrane confirmed that the membrane is non-toxic with suitable structural and biological properties for different clinical application. Beta-glucans manifests a broad spectrum of biological activities 5 and they are found in the cell wall of yeasts, fungi and pathogenic bacteria and also produced by a variety of plants.…”

Section: Discussionmentioning

confidence: 99%

“…Beta-glucans are polysaccharides consisting of a glucose residue jointed by beta linkage 3 and manifest a broad spectrum of biological activities: antimicrobial, anti-infective, anti-inflammatory, anti-arthritis, antihypertension, chemoprotective, antitumor, and immunostimulatory activity [4][5][6] . They are taken as herbal medicines, to prevent and treat cancer, lower cholesterol, for human immunodeficiency virus (HIV), and diabetes, and to increase the immune system function.…”

Section: Introductionmentioning

confidence: 99%

Biological properties of a novel coladerm-beta glucan membrane.In vitro assessment using human fibroblasts

Jantová¹,

Bakoš²,

Bírošová³

et al. 2015

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aim. The purpose of this study was to prepare a coladerm-beta glucan membrane (CBGM) and to evaluate its biocompatibility, cytotoxicity, antimicrobial activity, genotoxicity and mutagenicity. Methods. The biocompatibility of the membrane was studied on the base of cell adhesion and colonization of human fibroblasts on the biomaterial surface by light microscopy. The MTT test and LDH level determination in the culture medium removed from the control and cells treated on the membrane, were used for viability and cytotoxic evaluations. Flow cytometry and gel electrophoresis were used for analysis of cell cycle and death. The antimicrobial activity of CBGM was tested using the qualitative dilution method. Ames bacteria gene mutation test and Comet assay were used for mutagenic and genotoxic studies. Results. MTT and LDH tests confirmed that CBGM is a non-toxic biomaterial. Flow cytometry and gel electrophoresis demonstrated that the membrane did not affect the cell cycle and did not induce either necrotic or apoptotic cell death. CBGM exhibited antibacterial activity against G -bacteria E. sakazakii, S. marcescens, E. coli and agains G + sporogenic bacteria B. cereus. No antifungal activity was detected. The membrane did not induce mutagenicity in the bacterial reverse mutation test in Salmonella Typhimurium strains. Similarly, the comet assay showed that the tested fibroblast cells growing with/without the membrane did not show any statistically significant DNA damage. Conclusions. The CBGM has good biocompatibility, no cytotoxicity/genotoxicity/mutagenicity and it can be included as a potential scaffold for tissue engineering.

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Potentiating Effect of .BETA.-Glucans on Photodynamic Therapy of Implanted Cancer Cells in Mice

Cited by 15 publications

References 41 publications

Cellular and molecular effects of the liposomal mTHPC derivative Foslipos in prostate carcinoma cells in vitro

Cellular and molecular effects of the liposomal mTHPC derivative Foslipos in prostate carcinoma cells in vitro

Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects

Biological properties of a novel coladerm-beta glucan membrane.In vitro assessment using human fibroblasts

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