Potentiating Biosynthesis of Alkaloids and Polyphenolic Substances in Catharanthus roseus Plant Using ĸ-Carrageenan

Abstract

Catharanthus roseus is a medicinal plant that produces indole alkaloids, which are utilized in anticancer therapy. Vinblastine and vincristine, two commercially important antineoplastic alkaloids, are mostly found in the leaves of Catharanthus roseus. ĸ-carrageenan has been proven as plant growth promoting substance for a number of medicinal and agricultural plants. Considering the importance of ĸ-carrageenan as a promoter of plant growth and phytochemical constituents, especially alkaloids production in Catharanthus roseus, an experiment was carried out to explore the effect of ĸ-carrageenan on the plant growth, phytochemicals content, pigments content, and production of antitumor alkaloids in Catharanthus roseus after planting. Foliar application of ĸ-carrageenan (at 0, 400, 600 and 800 ppm) significantly improved the performance of Catharanthus roseus. Phytochemical analysis involved determining the amount of total phenolics (TP), flavonoids (F), free amino acids (FAA), alkaloids (TAC) and pigments contents by spectrophotometer, minerals by ICP, amino acids, phenolic compounds and alkaloids (Vincamine, Catharanthine, Vincracine (Vincristine), and vinblastine) analysis uses HPLC. The results indicated that all examined ĸ-carrageenan treatments led to a significant (p ≤ 0.05) increase in growth parameters compared to the untreated plants. Phytochemical examination indicates that the spray of ĸ-carrageenan at 800 mg L−1 increased the yield of alkaloids (Vincamine, Catharanthine and Vincracine (Vincristine)) by 41.85 μg/g DW, total phenolic compounds by 3948.6 μg gallic/g FW, the content of flavonoids 951.3 μg quercetin /g FW and carotenoids content 32.97 mg/g FW as compared to the control. An amount of 400 ppm ĸ-carrageenan treatment gave the best contents of FAA, Chl a, Chl b and anthocyanin. The element content of K, Ca, Cu, Zn and Se increased by treatments. Amino acids constituents and phenolics compounds contents were altered by ĸ-carrageenan.

Impact of biostimulants based amino acids and irrigation frequency on agro-physiological characteristics and productivity of broccoli plants

“…The enhancement of AsA, TPC, and total antioxidant content in sprayed plants with biostimulantsbased amino acids might be correlated to the improvement of the photosynthesis system in plants which leads to the accumulation of secondary metabolites. Furthermore, several authors confirmed that foliar application with amino acid bio-stimulant plays an important role in antioxidant metabolism in plants (Hildebrandt et al, 2015;Weiland et al, 2015;El-Beltagi et al, 2023;Ramadan et al, 2023).…”

Discovery of 3-Formyl-N-(un)Substituted Benzylindole Pyrimidines as an Acaricidal Agent and Their Mechanism of Action

“…The indole ( I-1 , R 1 = H, Figure ) scaffold is found in a wide range of bioactive heterocycles and natural products, such as vinblastine, vincristine, and plant growth regulator indolyl-3-acetic acid (IAA). − Indole as a natural alkaloid is isolated from the clavicipitaceous fungus Balansia epichloe (Weese), which parasitizes pasture grasses . It also occurs in the oil of jasmine, cloves, and coal tar .…”

Significance of selenium in ameliorating the effects of irrigation deficit via improving photosynthesis efficiency, cell integrity, osmo-protectants, and oil profile of anise crop

“…As well, it has antimicrobial, insecticidal, and antioxidative effects (Özcan and Chalchat, 2006;Afify et al, 2012) and carminative and mild expectorant characteristics of its essential oil were highlighted (El-Beltagi et al, 2020;Aly et al, 2023a). Furthermore, the role of anise oil in increasing the weight and the rate of conversion (FCR) of broiler hens was showed (El-Beltagi et al, 2023). Moreover, the aqueous extract of anise has a beneficial effect against neurological turbulence caused by lead intoxication (Ciftci et al, 2005).…”