Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia

Abstract

Summary:The development of novel markers usually requires high costs and is time consuming. Instead of developing new markers, their transfer within a genus is an alternative particularly suitable in less studied species. Hence the goal of this study was to evaluate the ability of SSR markers, previously developed from Vicia faba, and ISSR markers to estimate DNA polymorphism within the genus Vicia. Eight Vicia species were used for cross-species amplification. Transferability of SSR markers in the studied species ranged from 25% to 100%, with an average of 56.25%. Although three SSR primers were polymorphic, the nature of the amplification products indicated that their further usefulness is questionable. ISSR primers generated polymorphic products of amplification in all tested Vicia species. The average polymorphism of ISSR primers was 93.75%, which implies that these primers can be used within the genus Vicia.

Characterization of the Genetic Diversity of Some Species of Genus Vicia Using ISSR and ITS Molecular Techniques

“…The ISSR technique is known to be sensitive enough to detect low levels of genetic diversity while still having the benefits of ease and low cost. It can be used to detect the genetic diversity in Vicia, chickpea, Katsouni pea and Stylosanthes scabra (Sarwat, 2012;Singh et al, 2014;Laureles et al, 2015;Rajkovic′ et al, 2015;Costa et al, 2019;Stavridou et al, 2020) For more exact conclusions, DNA sequencing can be used as sequences are deemed to be largely stable throughout evolution and speciation. One sequence, that is, the internal transcribed spacer (ITS) region of ribosomal DNA, has provided promising results for phylogenetic analyses (Mishra et al, 2016).…”