Potential Use of IgG2 ELISA in the Diagnosis of Chronic Elephantiasis and IgG4 ELISA in the Follow-Up of Microfilaremic Patients Infected with Brugia malayi

Rahmah, N.; Anuar, A. Khairul; Karim, Rubina; Mehdi, R.; Sinniah, B; Omar, Ainsah

doi:10.1006/bbrc.1994.2650

Cited by 7 publications

(3 citation statements)

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“…Therefore, care should be taken to define the cut-off value to be employed in IgG4 assay for diagnosis of filaria infection. We have shown previously that filaria specific IgG4 antibody titres decreased significantly in individuals treated with diethylcarbamazine (Rahmah et al 1994). Kurniawan et al (1995) reported similar findings.…”

Section: Discussionsupporting

confidence: 78%

Use of antifilarial IgG4‐ELISA to detect Brugia malayi infection in an endemic area of Malaysia

Rahmah

et al. 1998

Tropical Med Int Health

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SummaryOBJECTIVE To evaluate the usefulness of antifilarial IgG4 antibody assay in detecting B. malayi infection in a filaria endemic area in Malaysia. METHODS A sandwich ELISA using B. malayi soluble antigen was employed to detect antifilarial IgG4 antibodies in serum samples of 330 individuals who comprised 88 healthy individuals from nonendemic areas, 15 B. malayi microfilaraemic cases, 22 individuals with soil-transmitted helminthiases, 9 elephantiasis cases and 196 residents from a B. malayi-endemic area. An O.D. value of Ͼ 0.420 at serum dilution of 1:400 was used as the cut-off point. This cut-off point was obtained by taking the mean optical density (0.252 ϩ 4 S.E.) of 36 negative sera which had O.D. values greater than 0.1 at serum dilution of 1: 400. RESULTS All 15 microfilaraemic persons were positive for antifilarial IgG4 antibody. Non-endemic normals, soil-transmitted helminth infected persons and chronic elephantiasis cases were negative for antifilarial IgG4 antibody. Of the 196 individuals from the filaria endemic area, 37 (18.8%) demonstrated presence of antifilarial IgG4 antibodies; and only eight individuals (4.1%) were positive for microfilariae. All eight microfilaraemic individuals were also positive for antifilarial IgG4 antibodies. CONCLUSION Antifilarial IgG4-ELISA could detect 4.6 times more positive cases than the microfilaria detection method. With appropriate cut-off values that eliminate cross-reactivities, this serological tool is very useful for Brugia malayi prevalence surveys and diagnosis.keywords antifilarial IgG4-ELISA, Brugia malayi, Malaysia correspondence Dr Rahmah Noordin,

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Section: Discussionsupporting

confidence: 78%

Use of antifilarial IgG4‐ELISA to detect Brugia malayi infection in an endemic area of Malaysia

Rahmah

et al. 1998

Tropical Med Int Health

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“…High levels of specific IgG (Suntharasamai et al 1985;Dharmkrong et al 1986) and IgE antibodies (Soesatyo et al 1987;Saksirisampant et al 2001) using G. spinigerum L3 crude extract in enzyme-linked immunosorbent assay (ELISA) have been shown with high sensitivity and reasonable specificity, but cross-reactivity with other parasites has not been eliminated. Specific IgG subclass antibodies have been shown to provide improved specificity over the total IgG antibody for the diagnosis of many parasitic infections, such as ascariasis (Chatterjee et al 1996), echinococcosis (Grimm et al 1998), leishmaniasis (Khairul et al 1999), and filariasis (Rahmah et al 1994;Triteeraprapab et al , 2001aNuchprayoon et al 2001). Such studies have not been carried out for gnathostomiasis.…”

Section: Introductionmentioning

confidence: 96%

Study of specific IgG subclass antibodies for diagnosis of Gnathostoma spinigerum

Nuchprayoon

Sanprasert

Suntravat

et al. 2003

Parasitology Research

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Gnathostoma spinigerum infection is endemic in Thailand and many Asian countries. Current diagnosis is the skin test and enzyme-linked immunosorbent assay (ELISA) for IgG antibody against the G. spinigerum third-stage larvae (L3), but cross-reactivity is common. We evaluated the sensitivity and specificity of anti-G. spinigerum L3 IgG subclass antibodies for diagnosis of 43 patients with gnathostomiasis. The majority of patients with gnathostomiasis (91%) had eosinophilia. While the anti-G. spinigerum L3 IgG1 antibody provided the highest sensitivity (98%), the anti-G. spinigerum L3 IgG2 antibody had the highest specificity (88%). The ELISA that detected anti-G. spinigerum L3 IgG1 antibody could be a reliable laboratory screening test, while anti-G. spinigerum L3 IgG2 antibody could be used to confirm the diagnosis.

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“…The IgG subclasses have been shown to provide improved specificity over the total IgG antibody array for the diagnosis of many parasitic infections, such as ascariasis, 16 echinococcosis, 17 leishmaniasis, 18 filariasis, 19,20 and gnathostomiasis caused by species of Gnathostoma other than G. binucleatum. 21,22 Therefore, the purpose of this study was to characterize the humoral immune response to a crude extract of G. binucleatum in patients with clinical diagnoses of gnathostomiasis to detect a possible antibody class or subclass that could be used in the diagnosis of gnathostomiasis.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

Zambrano‐Zaragoza¹,

Durán‐Avelar²,

Messina-Robles³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked freshwater fish infected with the advanced third-stage larvae of Gnathostoma spp. This disease is endemic to the Pacific region of Mexico, and one of its etiologic agents has been identified as Gnathostoma binucleatum. We characterized the humoral immune response of patients clinically diagnosed with gnathostomiasis by detecting total IgM, IgE, and IgG class and subclasses against a crude extract of the parasite by Western blotting. Our results do not show differences in the antigens recognized by IgM and IgE. However, we found that the specific humoral immune response is caused mainly by IgG, specifically IgG4. We found that 43%, 65.2%, 54.1%, and 26.3% of the patients recognize the 37-kD, 33-kD, 31-kD, and 24-kDa antigens, suggesting that the 33-kD antigen is the immunodominant antigen of G. binucleatum.

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Potential Use of IgG2 ELISA in the Diagnosis of Chronic Elephantiasis and IgG4 ELISA in the Follow-Up of Microfilaremic Patients Infected with Brugia malayi

Cited by 7 publications

References 0 publications

Use of antifilarial IgG4‐ELISA to detect Brugia malayi infection in an endemic area of Malaysia

Use of antifilarial IgG4‐ELISA to detect Brugia malayi infection in an endemic area of Malaysia

Study of specific IgG subclass antibodies for diagnosis of Gnathostoma spinigerum

Characterization of the Humoral Immune Response against Gnathostoma binucleatum in Patients Clinically Diagnosed with Gnathostomiasis

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