Potential Use of Collagen-Chitosan-Hyaluronan Tri-Copolymer Scaffold for Cartilage Tissue Engineering

Abstract

A three-dimensional biodegradable porous scaffold plays a vital role in a tissue engineering approach. Collagen, chitosan and hyaluronan (HA) are natural extracellular matrix (ECM) or similarity, and may provide appropriate environment for the generation of cartilage-like tissue. In this study, we prepared a collagen/chitosan/HA tri-copolymer porous scaffold by freezing and lyophilization to evaluate physico-chemical properties of the tri-copolymer scaffold and its capacity to sustain chondrocytes proliferation and differentiation in vitro. The results show that the mechanical strength, the resistance to enzymatic degradation, and the waterblinding capacity were improved when chitosan and hyaluronan were incorporated into a collagen scaffold. After 21 days of culture, the porous scaffold had been surfaced with cartilaginous tissue. DNA and glycosaminoglycan (GAG) contents were significantly higher during culture periods in collagen/ chitosan/hyaluronan matrix compared to collagen alone matrix, and most seeded cells preserved the chondrocytic phenotype during culture within the scaffold. The collagen/chitosan/hyaluronan tri-copolymer scaffold has potential applications in a cartilage tissue engineering scaffold field.

Marine Streptomyces as a novel source of bioactive substances

“…Chitin, a linear b-1,4-linked homopolymer of N-acetylglucosamine, is one of the three most abundant polysaccharides in nature besides cellulose and starch. The antifungal activity and highly biocompatible quality make chitinase and its derivatives particularly useful for biomedical applications, such as wound healings, cartilage tissue engineering, drug delivery, and nerve generation (Shi et al 2006;Yan et al 2006). The biodegradable and antifungal properties of chitinase are also useful for environmental and agricultural uses, food technology and cosmetics (Goosen 1997;Rabea et al 2003;Lin and Lin 2005).…”

Determination of mechanical properties of soft tissue scaffolds by atomic force microscopy nanoindentation

“…A variety of biomaterials have been developed and adopted for fabricating 3D scaffolds. Collagen and chitosan are two widely used natural biomaterials for 3D cultures (Chen et al, 2006;Ma et al, 2003;Yan et al, 2006;Zhang et al, 2006). Both materials can be readily cross-linked to form hydrogels for growing soft tissues (von Heimburg et al, 2001;Wang and Ye, 2009;Zustiak and Leach, 2010).…”

Construcción de matrices como sustitutos dérmicos: aplicación potencial en la regeneración de piel (MATRIX CONSTRUCTION AS DERMAL SUBSTITUTES: POTENTIAL APPLICATION IN SKIN REGENERATION)

“…y el Col aislado de piel de ternero (Sigma, EE.UU.) fueron disueltos al 1% (P/V) en una solución de ácido acético al 0,3% por separado, mediante agitación magnética a 4°C [12,13]. Las diferentes concentraciones con combinaciones de AH y Col se realizaron en proporciones de Col:AH de 10:0, 4:6, 2:8 y 0:10 para analizarlas y hacer comparaciones entre sí.…”