Determination of mechanical properties of soft tissue scaffolds by atomic force microscopy nanoindentation

Zhu, Yanxia; Dong, Zhuxin; Wejinya, Uchechukwu C.; Jin, Sha; Ye, Kaiming

doi:10.1016/j.jbiomech.2011.07.010

Cited by 86 publications

(69 citation statements)

References 37 publications

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“…Hydrogel scaffolds derived from ECM proteins tend to have lower elastic moduli that more closely match the elasticity of hPSCs. 48 We have also found that the elastic modulus of collagen I hydrogels can be modulated by the addition of chitosan. These softer scaffolds may find use in differentiation protocols where undifferentiated cells rather than partially differentiated EB-derived cells are seeded into the scaffolds.…”

Section: Mechanical Properties Of Substrates Can Regulate Hpsc Fatementioning

confidence: 66%

Mechanobiology of Human Pluripotent Stem Cells

Earls

Jin

2013

Tissue Engineering Part B: Reviews

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Human pluripotent stem cells (hPSCs) are self-renewing and have the potential to differentiate into any cell type in the body, making them attractive cell sources for applications in tissue engineering and regenerative medicine. However, in order for hPSCs to find use in the clinic, the mechanisms underlying their self-renewal and lineage commitment must be better understood. Many technologies that have been developed for the maintenance and directed differentiation of hPSCs involve the use of soluble growth factors, but recent studies suggest that other elements of the hPSC microenvironment also influence the growth and differentiation of hPSCs. This includes the influences of cell-cell interactions, substrate mechanics, cellular interactions with extracellular matrix, as well as the nanotopography of the substrate and physical forces such as shear stress, cyclic mechanical strain, and compression. In this review, we highlight the recent progress of this area of research and discuss ways in which the mechanical cues may be incorporated into hPSC culture regimes to improve methods for expanding and differentiating hPSCs.

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Section: Mechanical Properties Of Substrates Can Regulate Hpsc Fatementioning

confidence: 66%

Mechanobiology of Human Pluripotent Stem Cells

Earls

Jin

2013

Tissue Engineering Part B: Reviews

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“…The Young's modulus was determined by probing the hydrated gel and calculating the stiffness based on cantilever deflection [52]. Bruker DNP-D probes were used in an aqueous environment.…”

Section: Calcium Alginate Hydrogel Fabrication and Characterizationmentioning

confidence: 99%

“…The spring constant of the probes were first calibrated by measuring the thermal noise and fitting the response of a simple harmonic oscillator in fluid. The sample stiffness was measured by fitting probe deflection curves according to the Sneddon model and eliminating close range sample-probe interactions [52,53].…”

Section: Calcium Alginate Hydrogel Fabrication and Characterizationmentioning

confidence: 99%

Digital Microfluidic System with Vertical Functionality

Bender

Garrell

2015

Micromachines

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Digital (droplet) microfluidics (DµF) is a powerful platform for automated lab-on-a-chip procedures, ranging from quantitative bioassays such as RT-qPCR to complete mammalian cell culturing. The simple MEMS processing protocols typically employed to fabricate DµF devices limit their functionality to two dimensions, and hence constrain the applications for which these devices can be used. This paper describes the integration of vertical functionality into a DµF platform by stacking two planar digital microfluidic devices, altering the electrode fabrication process, and incorporating channels for reversibly translating droplets between layers. Vertical droplet movement was modeled to advance the device design, and three applications that were previously unachievable using a conventional format are demonstrated: (1) solutions of calcium dichloride and sodium alginate were vertically mixed to produce a hydrogel with a radially symmetric gradient in crosslink density; (2) a calcium alginate hydrogel was formed within the through-well to create a particle sieve for filtering suspensions passed from one layer to the next; and (3) a cell spheroid formed using an on-chip hanging-drop was retrieved for use in downstream processing. The general capability of vertically delivering droplets between multiple stacked levels represents a processing innovation that increases DµF functionality and has many potential applications.

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“…AFM is well known not only for its high resolution but also for detection of mechanical properties based on the interaction force between a microscopic tip and a sample surface (Shi et al, 2009). In 2011, Zhu obtained the mechanical properties of soft tissue scaffolds by AFM nanoindentation, the elastic modulus of a scaffold composed of 70% collagen and 30% chitosan was about 11.6 KPa (Zhu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Mechanical properties study of SW480 cells based on AFM

Liu

Song

et al. 2015

Cell Biology International

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Since the invention of the atomic force microscope (AFM), it has been widely applied in biomedicine. One of the most important applications is used as an indenter tool to do the indentation experiment in order to get the mechanical properties of cells. In this paper, SW480 cells were used as the test subjects. Through the analysis of the contact and indentation, Young's modulus (E), which is an important parameter of cancer cells, has been estimated. Experimental results show that different mechanical models should be chosen to calculate the E in different indentation depths. Here, the E of SW480 cells was (2.5 ± 0.8) KPa at the indentation depth of 99 nm.

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Determination of mechanical properties of soft tissue scaffolds by atomic force microscopy nanoindentation

Cited by 86 publications

References 37 publications

Mechanobiology of Human Pluripotent Stem Cells

Mechanobiology of Human Pluripotent Stem Cells

Digital Microfluidic System with Vertical Functionality

Mechanical properties study of SW480 cells based on AFM

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