Potential tumor suppressing role of microRNA-545 in epithelial ovarian cancer

Jia, Xibiao; Liu, Xiaogang; Li, Ming; Zeng, Yu; Feng, Zaoming; Su, Xian; Huang, Yan; Chen, Maomao; Yang, Xueyi

doi:10.3892/ol.2018.8130

Cited by 16 publications

(24 citation statements)

References 38 publications

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“…In EOC, miR-545-3p is downregulated in EOC tissues and cell lines [34]. Patients with EOC characterized by low miR-545-3p expression have a shorter overall survival compared with patients exhibiting high miR-545-3p expression [34]. Consistent with this result, the decrease of miR-545-3p expression in EOC was veri ed in our study and its upregulation suppressed cell growth and metastasis by directly targeting HDAC4.…”

Section: Discussionsupporting

confidence: 87%

“…Differentially expressed miR-545-3p has been observed in diverse human malignancies [46][47][48]. In EOC, miR-545-3p is downregulated in EOC tissues and cell lines [34]. Patients with EOC characterized by low miR-545-3p expression have a shorter overall survival compared with patients exhibiting high miR-545-3p expression [34].…”

Section: Discussionmentioning

confidence: 99%

“…A total of 14 miRNAs ( Figure 2D) were predicted by both miRDB and StarBase 3.0. Ten miRNAs, including miR-23a-3p [26], miR-23b-3p [27], miR-23c [28], miR-340-5p [29], miR-374c-5p [30], miR-376c-3p [31], miR-383-5p [32], miR-532-5p [33], miR-545-3p [34], and miR-655-3p [35,36], were selected as candidates for subsequent experiments, in light of their implication in cancer oncogenicity.…”

Section: Ptprg-as1 Functions As a Mir-545-3p Sponge In Eoc Cellsmentioning

confidence: 99%

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Long Non-coding RNA PTPRG-AS1 Promotes Cell Tumorigenicity in Epithelial Ovarian Cancer by Decoying microRNA-545-3p and Consequently Enhancing HDAC4 Expression

Shi

Zhang

et al. 2020

Preprint

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Background: Long non-coding RNA PTPRG antisense RNA 1 (PTPRG-AS1) deregulation has been reported in various human malignancies and identified as an important modulator of cancer development. Few reports have focused on the detailed role of PTPRG-AS1 in epithelial ovarian cancer (EOC) and its underlying mechanism. This study aimed to determine the physiological function of PTPRG-AS1 in EOC. A series of experiments were also done to identify the mechanisms through which PTPRG-AS1 exerts its function in EOC.Methods: Reverse transcription-quantitative polymerase chain reaction was used to determine PTPRG-AS1 expression in EOC tissues and cell lines. PTPRG-AS1 was silenced in EOC cells and studied with respect to cell proliferation, apoptosis, migration, invasion in vitro, and tumor growth in vivo. The putative miRNAs that target PTPRG-AS1 were predicted using bioinformatics analysis and further confirmed in luciferase reporter and RNA immunoprecipitation assays.Results: Our data verified the upregulation of PTPRG-AS1 in EOC tissues and cell lines. High PTPRG-AS1 expression was associated with shorter overall survival in patients with EOC. Functionally, EOC cell proliferation, migration, invasion in vitro and tumor growth in vivo were suppressed by PTPRG-AS1 silencing. In contrast, cell apoptosis was promoted by loss of PTPRG-AS1. For the mechanism part, PTPRG-AS1 could serve as a competing endogenous RNA in EOC cells by decoying microRNA-545-3p (miR-545-3p), thereby elevating histone deacetylase 4 (HDAC4) expression. Furthermore, rescue experiments revealed that PTPRG-AS1 knockdown-mediated effects on EOC cells were, in part, counteracted by the inhibition of miR-545-3p or restoration of HDAC4.Conclusions: PTPRG-AS1 functioned as an oncogenic lncRNA that aggravated the malignancy of EOC through the miR-545-3p/HDAC4 ceRNA network. Thus, targeting the PTPRG-AS1/miR-545-3p/HDAC4 pathway may be a novel strategy for EOC anticancer therapy.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Ptprg-as1 Functions As a Mir-545-3p Sponge In Eoc Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Long Non-coding RNA PTPRG-AS1 Promotes Cell Tumorigenicity in Epithelial Ovarian Cancer by Decoying microRNA-545-3p and Consequently Enhancing HDAC4 Expression

Shi

Zhang

et al. 2020

Preprint

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“…With the environmental deterioration and other factors, a younger trend of breast cancer occurrence has been shown in recent years 20 The radical surgical resection, combined with radiotherapy and chemotherapy, marks the improvement regarding the survival time of early breast cancer patients, but its therapeutic effect is not satisfactory on advanced breast cancer patients 21 miRNA, as a kind of endogenous small noncoding RNA, plays an important role in cell differentiation, proliferation, and apoptosis through regulating the signaling pathway. Accumulative evidences indicate that a variety of miRNAs are involved in the regulation process of several tumors and play similar roles to tumor suppressor genes and oncogenes 22,23 The abnormal expressions of miR-21 and miR-205 are possibly related to the occurrence and development of breast cancer 12 Li et al 24 found that the miR-205 expression level is increased in epithelial ovarian cancer, and is closely related to the proliferation and invasion of ovarian cancer. In this study, we found that the level of miR-205-3p was significantly upregulated in breast cancer tissues, and further in vitro assay by overexpressing miR-205-3p validated its role on proliferation, invasion, and apoptosis of cancer cells, which was in line with previous findings 24 .…”

Section: Discussionmentioning

confidence: 99%

“…at the age of 45-65 years. Accumulative evidences indicate that a variety of miRNAs are involved in the regulation process of several tumors and play similar roles to tumor suppressor genes and oncogenes22,23 The abnormal expressions of miR-21 and miR-205 are possibly related to the occurrence and development of breast cancer12 Li et al 24 found that the miR-205 expression level is increased in epithelial ovarian cancer, and is closely related to the proliferation and invasion of ovarian cancer. Accumulative evidences indicate that a variety of miRNAs are involved in the regulation process of several tumors and play similar roles to tumor suppressor genes and oncogenes22,23 The abnormal expressions of miR-21 and miR-205 are possibly related to the occurrence and development of breast cancer12 Li et al 24 found that the miR-205 expression level is increased in epithelial ovarian cancer, and is closely related to the proliferation and invasion of ovarian cancer.…”

mentioning

confidence: 99%

miR‐205‐3p promotes proliferation and reduces apoptosis of breast cancer MCF‐7 cells and is associated with poor prognosis of breast cancer patients

Qiu

Huang

Zhang

et al. 2019

Clinical Laboratory Analysis

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Background To study the expression of microribonucleic acid (miR)‐205 in breast cancer and its effects on the proliferation and apoptosis of breast cancer cells. Methods Breast cancer cell line MCF‐7 cells with stable expression of miR‐205‐3p were constructed. Cell proliferation, invasion, and apoptosis were detected via MTT assay, transwell assay, and flow cytometry, respectively. The expressions of Ezrin, LaminA/C, cleaved caspase‐3, Bcl‐2, and Bax were detected via Western blotting. The expressions of miR‐205‐3p in breast cancer tissues and para‐carcinoma tissues were detected via quantitative PCR (qPCR). Results In transfection group, cell proliferation and invasion capacities were increased significantly (P < 0.01), but apoptotic cells were significantly reduced (P < 0.01). In addition, the expressions of Ezrin, LaminA/C, and cleaved caspase‐3 in the transfection group were significantly decreased (P < 0.01), but the Bcl‐2/Bax ratio was significantly increased (P < 0.01). The miR‐205‐3p expression in tumor tissues of breast cancer patients was significantly higher than that in para‐carcinoma tissue, but Ezrin, LaminA/C, and cleaved caspase‐3 expressions in tumor tissues were remarkably declined (P < 0.01), while the Bcl‐2/Bax ratio was remarkably increased (P < 0.01). Moreover, the 5‐year survival of patients with high expression of miR‐205‐3p was significantly shorter than patients with normal or low expression (P < 0.01). Conclusion Highly expressed miR‐205‐3p can promote the proliferation and invasion and reduce the apoptosis of breast cancer cells, and the high expression of miR‐205‐3p can significantly reduce the survival time of patients.

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Overexpressed circ_0067934 acts as an oncogene to facilitate cervical cancer progression via the miR‐545/EIF3C axis

Wang

et al. 2018

Journal Cellular Physiology

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Circular RNAs were recently identified as a novel type of noncoding RNAs. An increasing number of reports have demonstrated their essential regulatory roles in various biological processes and human diseases, including cancer. However, the role of circRNA in cervical cancer (CC) remains largely unknown. In the current study, we investigated the physiological functions of circ_0067934 during CC development and progression. We found that circ_0067934 was overexpressed in CC tissues and cell lines. Circ_0067934 upregulation was associated with advanced stage, lymph node metastasis, and poor prognosis in CC patients. Knockdown of circ_0067934 suppressed the proliferation, colony formation, migration, invasion, and epithelial‐mesenchymal transition of CC cells in vitro. Circ_0067934 loss also inhibited CC tumor growth in vivo. Mechanistically, silencing circ_0067934 increased miR‐545 expression. MiR‐545 repressed EIF3C expression through targeting its 3′‐untranslated region. MiR‐545 suppressed the proliferation, migration, and invasion of CC cells, whereas restoration of EIF3C could rescue the effects of circ_0067934 knockdown. Taken together, our findings revealed that circ_0067934 promotes CC progression via miR‐545/EIF3C axis. Our study may provide a new insight into the pathogenesis of CC.

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Potential tumor suppressing role of microRNA-545 in epithelial ovarian cancer

Cited by 16 publications

References 38 publications

Long Non-coding RNA PTPRG-AS1 Promotes Cell Tumorigenicity in Epithelial Ovarian Cancer by Decoying microRNA-545-3p and Consequently Enhancing HDAC4 Expression

Long Non-coding RNA PTPRG-AS1 Promotes Cell Tumorigenicity in Epithelial Ovarian Cancer by Decoying microRNA-545-3p and Consequently Enhancing HDAC4 Expression

miR‐205‐3p promotes proliferation and reduces apoptosis of breast cancer MCF‐7 cells and is associated with poor prognosis of breast cancer patients

Overexpressed circ_0067934 acts as an oncogene to facilitate cervical cancer progression via the miR‐545/EIF3C axis

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