Potential testing of reprocessing procedures by real-time polymerase chain reaction: A multicenter study of colonoscopy devices

Valeriani, Federica; Agodi, Antonella; Casini, Beatrice; Cristina, Maria Luisa; D’Errico, Marcello Mario; Gianfranceschi, Gianluca; Liguori, Giorgio; Liguori, Renato; Mucci, N; Mura, I; Pasquarella, Cesira; Piana, Andrea; Sotgiu, Giovanni; Privitera, Gaetano Pierpaolo; Protano, Carmela; Quattrocchi, Annalisa; Ripabelli, Giancarlo; Rossini, Angelo; Spagnolo, Anna Maria; Tamburro, Manuela; Tardivo, Stefano; Veronesi, Licia; Vitali, Matteo; Spica, Vincenzo Romano

doi:10.1016/j.ajic.2017.08.008

Cited by 21 publications

(19 citation statements)

References 27 publications

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“…A fecal swab (Copan Italia S.P.A., Brescia, Italy) was given to each participant, together with the instructions for the collection of a stool sample; participants were asked to deliver the swab on a planned day within two hours since collection. Samples were then stored at 4-8 • C in a refrigerated container and were taken within 24 h to the laboratory of the University of Rome "Foro Italico", where they were processed with a previously validated protocol for DNA extraction from fecal traces [23]. Feces were weighed prior to the extraction, and DNA was purified and normalized; the libraries for next-generation sequencing (NGS) were prepared according to the 16S Metagenomic Sequencing Library Preparation Guide (part# 15044223 rev A; Illumina, San Diego, CA, USA).…”

Section: Analysis Of Fecal Samplesmentioning

confidence: 99%

Mediterranean Diet, Physical Activity and Gut Microbiome Composition: A Cross-Sectional Study among Healthy Young Italian Adults

Gallé

Valeriani

Cattaruzza³

et al. 2020

Nutrients

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Background. This cross-sectional study aimed to explore the microbial composition of the gut and its possible association with the Mediterranean diet (MD) after adjusting for demographic and anthropometric characteristics in a sample of healthy young Italian adults. Methods. Gut microbiota, demographic information, and data on adherence to MD and physical activity (PA) habits were collected in a sample of 140 university students (48.6% males, mean age 22.5 ± 2.9) with a mean body mass index (BMI) of 22.4 ± 2.8 kg/m2 (15.2–33.8) and a mean PA level of 3006.2 ± 2973.6 metabolic equivalent (MET)-minutes/week (148–21,090). Results. A high prevalence of Firmicutes and Bacteroidetes was found in all the fecal samples. Significant dissimilarities in the microbiota composition were found on the basis of MD adherence and PA levels (p = 0.001). At the genus level, Streptococcus and Dorea were highly abundant in overweight/obese individuals, Ruminococcus and Oscillospira in participants with lower adherence to MD, and Lachnobacterium in subjects with low levels of PA (p = 0.001). A significantly higher abundance of Paraprevotella was shown by individuals with lower BMI, lower MD adherence, and lower PA levels (p = 0.001). Conclusions. This study contributes to the characterization of the gut microbiome of healthy humans. The findings suggest the role of diet and PA in determining gut microbiota variability.

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Section: Analysis Of Fecal Samplesmentioning

confidence: 99%

Mediterranean Diet, Physical Activity and Gut Microbiome Composition: A Cross-Sectional Study among Healthy Young Italian Adults

Gallé

Valeriani

Cattaruzza³

et al. 2020

Nutrients

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“…After sample collection, each swab was inserted into the semipermeable NAO Baskets and was broken inside at the breakpoint. The samples were pretreated with glass beads® (Sigma Aldrich, USA) and 200 μl of Lysozyme Solution® (Sigma Aldrich, USA), adapting the protocol as previous described (Valeriani et al., 2017, 2018a and b). In a second phase we followed the standard protocol procedure of GenElute® Bacterial Genomic DNA Kit (Sigma Aldrich, USA).…”

Section: Methodsmentioning

confidence: 99%

Artificial-turf surfaces for sport and recreational activities: microbiota analysis and 16S sequencing signature of synthetic vs natural soccer fields

Valeriani

Margarucci

Gianfranceschi

et al. 2019

Heliyon

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Synthetic fibres are used in place of the natural grass worldwide, for realizing playgrounds, soccer fields and even domestic gardens or recreational structures. An intensive use of artificial turf is currently observed in sports facilities, due to lower costs, higher sustainability in recycling of materials, and advantages related to athletic practice and performance. However, even if chemical and physical risks were studied, the microbiological component was not fully addressed, especially considering a comprehensive evaluation of the microbiota in synthetic vs natural playground surfaces. Here, we investigated the microbial community present on soccer fields, using Next Generation Sequencing and a 16S amplicon sequencing approach. Artificial and natural turfs show own ecosystems with different microbial profiles and a mean Shannon's diversity value of 2.176 and 2.475, respectively. The bacterial community is significantly different between facilities (ANOSIM: R ¼ 0.179; p < 0.001) and surface materials (ANOSIM: R ¼ 0.172; p < 0.005). The relative abundance of potentially pathogenic bacterial OTUs was higher in synthetic than in natural samples (ANOVA, F ¼ 2.2). Soccer fields are characterized by their own microbiota, showing a different 16S amplicon sequencing signature between natural and artificial turfs.

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“…The approach, which the authors described as easy to apply, had a sensitivity and specificity of 98 percent for identifying contaminated endoscopes. Full analysis only took three or four hours compared to the 24 to 72 hours required with cultures . According to Armellino, although surveillance methods can be beneficial from an assessment standpoint, they may not be practical.…”

Section: Quality Monitoringmentioning

confidence: 99%

Key steps in flexible endoscope reprocessing

Croke¹

2019

AORN Journal

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Potential testing of reprocessing procedures by real-time polymerase chain reaction: A multicenter study of colonoscopy devices

Cited by 21 publications

References 27 publications

Mediterranean Diet, Physical Activity and Gut Microbiome Composition: A Cross-Sectional Study among Healthy Young Italian Adults

Mediterranean Diet, Physical Activity and Gut Microbiome Composition: A Cross-Sectional Study among Healthy Young Italian Adults

Artificial-turf surfaces for sport and recreational activities: microbiota analysis and 16S sequencing signature of synthetic vs natural soccer fields

Key steps in flexible endoscope reprocessing

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