Artificial-turf surfaces for sport and recreational activities: microbiota analysis and 16S sequencing signature of synthetic vs natural soccer fields

Valeriani, Federica; Margarucci, Lory Marika; Gianfranceschi, Gianluca; Ciccarelli, Antonello; Tajani, Filippo; Mucci, N; Ripani, Maurizio; Spica, Vincenzo Romano

doi:10.1016/j.heliyon.2019.e02334

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…The use of molecular technologies is fundamental to understanding the structure and functions of microbiota and nonculturable microorganisms (Valeriani et al, 2019). Advances in genomics and metagenomics, marker genes, 16S rRNA gene sequencing and other molecular approaches are essential for predicting soil microbiome functions and improving agriculture production (Fierer, 2017).…”

Section: Introductionmentioning

confidence: 99%

Structuring biofilm communities living in pesticide contaminated water

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Moreira

Freitas

et al. 2020

Heliyon

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The wide use of pesticides in agriculture expose microbiota to stressful conditions that require the development of survival strategies. The bacterial response to many pollutants has not been elucidated in detail, as well as the evolutionary processes that occur to build adapted communities. The purpose of this study was to evaluate the bacterial population structure and adaptation strategies in planktonic and biofilm communities in limited environments, as tanks containing water used for washing herbicide containers. This biodiversity, with high percentage of nonculturable microorganisms, was characterized based on habitat and abiotic parameters using molecular and bioinformatics tools. According to water and wastewater standards, the physicochemical conditions of the tank water were inadequate for survival of the identified bacteria, which had to develop survival strategies in this hostile environment. The biodiversity decreased in the transition from planktonic to biofilm samples, indicating a possible association between genetic drift and selection of individuals that survive under stressful conditions, such as heating in water and the presence of chlorine, fluorine and agrochemicals over a sixmonth period. The abundance of Enterobacter, Acinetobacter and Pseudomonas in biofilms from water tanks was linked to essential processes, deduced from the genes attributed to these taxonomic units, and related to biofilm formation, structure and membrane transport, quorum sensing and xenobiotic degradation. These characteristics were randomly combined and fixed in the biofilm community. Thus, communities of biofilm bacteria obtained under these environmental conditions serve as interesting models for studying herbicide biodegradation kinetics and the prospects of consortia suitable for use in bioremediation in reservoirs containing herbicide-contaminated wastewater, as biofilters containing biofilm communities capable of degrading herbicides.

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Section: Introductionmentioning

confidence: 99%

Structuring biofilm communities living in pesticide contaminated water

Lima

Moreira

Freitas

et al. 2020

Heliyon

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“…amplifying bacterial genes from E. salivarius and S. mutans for targeting saliva, or Corynebacterium for nose secretions, or E. faecalis and Bacteroides for evaluating the presence of faecal traces, as previously shown (Esberg et al, 2020; Liu et al, 2020; Charles et al, 2019; Proctor et al, 2019; Lloyd-Price, J et al, 2016). This approach can be confirmed by next generation sequencing (NGS), analysing the whole microflora DNA (mfDNA) as sampled with environmental swabs on indoor and outdoor surfaces (Slatko et al, 2018; Valeriani et al, 2018b; Valeriani et al, 2018c; Valeriani et al, 2019; Mucci et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Monitoring COVID-19 transmission risks by RT-PCR tracing of droplets in hospital and living environments

Piana¹,

Colucci

Valeriani³

et al. 2020

Preprint

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SARS-CoV-2 environmental contamination occurs through droplets and biological fluids released in the surroundings from patients or asymptomatic carriers. Surfaces and objects contaminated by saliva or nose secretions represent a risk for indirect transmission of COVID-19. We assayed surfaces from hospital and living spaces to identify the presence of viral RNA and the spread of fomites in the environment. Anthropic contamination by droplets and biological fluids was monitored by detecting the microbiota signature using multiplex RT-PCR on selected species and massive sequencing on 16S-amplicons. A total of 92 samples (flocked swab) were collected from critical areas during the pandemic, including indoor (3 hospitals and 3 public buildings) and outdoor surfaces exposed to anthropic contamination (handles and handrails, playgrounds). Traces of biological fluids were frequently detected in spaces open to the public and on objects that are touched with the hands (>80%). However, viral RNA was not detected in hospital wards or other indoor and outdoor surfaces either in the air system of a COVID-hospital, but only in the surroundings of an infected patient, in consistent association with droplets traces and fomites. Handled objects accumulated the highest level of multiple contaminations by saliva, nose secretions and faecal traces, further supporting the priority role of handwashing in prevention. In conclusion, anthropic contamination by droplets and biological fluids is widespread in spaces open to the public and can be traced by RT-PCR. Monitoring fomites can support evaluation of indirect transmission risks for Coronavirus or other flu-like viruses in the environment.

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Evaluating the impact of turf‐care products on soil biological health

et al. 2020

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Golf courses require extensive use of inputs to meet the needs of playability and aesthetics. The impact of these inputs on soil biological health is largely unknown. Two field trials were conducted at a golf course in Georgia to evaluate short‐term effects of wetting agents (Cascade Plus and Duplex [C+D], Revolution [Rev]), plant growth regulators (PrimoMaxx [PM] and Cutless [CL]), and a product called PlantHelper (PH) on soil biological health by measuring microbial abundance and function. Quantitative polymerase chain reaction was used to measure microbial abundance, which included total bacteria, total fungi, and ammonia‐oxidizing prokaryotes. Soil respiration and enzyme assays were used as additional indicators of soil health. In bentgrass putting green, total bacteria and ammonia‐oxidizing bacteria decreased in abundance in response to the wetting agents and PH, indicating their sensitivity to the products. Whereas C+D stimulated urease activity, Rev and PH caused a short‐lived but immediate increase in respiration, indicating that they acted as labile carbon sources. In a bermudagrass fairway, PM was the only product that caused an increase in total bacteria abundance. PrimoMaxx and CL caused a delayed increase in respiration, suggesting that they may have affected the microorganisms indirectly through their impact on root growth and exudate production later. Although CL caused a decrease in urease activity, none of the products significantly affected phosphatase activity. Overall, the products did not seem to have a lasting impact on soil biological health, although long‐term studies are needed to confirm these observations.

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Artificial-turf surfaces for sport and recreational activities: microbiota analysis and 16S sequencing signature of synthetic vs natural soccer fields

Cited by 4 publications

References 37 publications

Structuring biofilm communities living in pesticide contaminated water

Structuring biofilm communities living in pesticide contaminated water

Monitoring COVID-19 transmission risks by RT-PCR tracing of droplets in hospital and living environments

Evaluating the impact of turf‐care products on soil biological health

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