Potential Skin Regeneration Activity and Chemical Composition of Absolute from Pueraria thunbergiana Flower

Won

Chemistry & Biodiversity

et al. 2020

Self Cite

Paederia foetida (PF) has antidiarrheal, antidiabetic, and anti-inflammatory activities. However, its biological activities on skin remain unclear. In this study, we examined the effect of PF flower absolute (PFFA) on skin wound healing-and skin barrier-linked responses in human epidermal keratinocytes (HaCaT cells). PFFA contained 23 components and increased the proliferation and sprout outgrowth of HaCaT cells and modestly increased migration. PFFA enhanced the phosphorylation levels of extracellular signal-regulated kinase1/2, serine/threonine-specific protein kinase (AKT), and p38 mitogen-activated protein kinase (MAPK) in HaCaT cells, and upregulated type I and IV collagen synthesis and filaggrin (an epidermal barrier protein) expression in HaCaT cells. These findings suggest PFFA may promote skin wound repair by stimulating migratory and proliferative activities (probably through the AKT/MAPK pathway), collagen synthesis, and skin barrier repair by upregulating the expressions of filaggrin in epidermal keratinocytes. Therefore, PFFA may be useful for developing agents that enhance skin wound and barrier-repair functions.

Section: Effect Of Pffa On Hacat Cell Sprout Outgrowthmentioning

confidence: 99%

“…[19 -23] Several recent studies have demonstrated that plants and their extracts can improve skin wound and barrier repair functions. [24][25][26][27][28] However, PF flower absolute has not been previously investigated in this context.…”

Section: Introductionmentioning

confidence: 99%

Stimulatory Effects ofPaederia foetidaFlower Absolute on the Skin Wound and Barrier Repair Activities of Keratinocytes

Won

Chemistry & Biodiversity

et al. 2020

Self Cite

“…This promotes skin proinflammatory activity and epithelialization, resulting in skin regeneration and wound healing. Kim and his Korean team conducted their research on isoflavonoids from the flower of kudzu plant, whose skin-regenerating effect was still unknown at that time [36]. They investigated the skin-regenerative potential of isoflavonoids in human epidermal keratinocytes (HaCats) and found that it induced type I and IV collagen synthesis in HaCats, and the treatment with these isoflavonoids increased sprout outgrowth in HaCats [36].…”

Section: Skin Regeneration Activitymentioning

confidence: 99%

“…Kim and his Korean team conducted their research on isoflavonoids from the flower of kudzu plant, whose skin-regenerating effect was still unknown at that time [36]. They investigated the skin-regenerative potential of isoflavonoids in human epidermal keratinocytes (HaCats) and found that it induced type I and IV collagen synthesis in HaCats, and the treatment with these isoflavonoids increased sprout outgrowth in HaCats [36]. The results suggest that these phytochemical compounds may participate in skin regeneration by promoting migration, proliferation, and collagen synthesis.…”

Section: Skin Regeneration Activitymentioning

confidence: 99%

A Promising View of Kudzu Plant, Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep: Flavonoid Phytochemical Compounds, Taxonomic Data, Traditional Uses and Potential Biological Activities for Future Cosmetic Application

Intharuksa

Sasaki

2020

Cosmetics

Pueraria montana var. lobata is widely known as kudzu especially in Japan, China, Korea, and other Asian countries. This plant is an ingredient for traditional food and an herbal ingredient for traditional medicines, particular in Japan and China. There are a few reports on its cosmetic uses. Interestingly, many phytochemical compounds from this plant have been continuously reported, particularly flavonoid compounds, which are well-known as potential bioactive ingredients for cosmetics. This work aims to illustrate promising views of kudzu plant, focusing on the diversity of flavonoid phytochemical compounds, taxonomic data, traditional uses, and potential biological activities for future cosmetic applications, i.e., antioxidant, antiglycation, skin regeneration, and melanogenesis inhibitory activities.

“…The constituent parts of this plant contain isoflavones, such as puerarin, daidzin, genistin, daidzein, and genistein . The extract of P. lobata has shown a wide range of beneficial effects, including antioxidant/anti‐diabetic effects, weight loss, collagen synthesis, anti‐melanogenic effects, anti‐aging/moisturizing effects, and procollagen formation . Thus, fermentation of the P. lobata extract (FPE) with the equol‐producing aerobic strain L. paracasei JS1 was expected to increase the efficacy of the extract, because equol and its precursors are abundant with the P. lobata extract (PE) and equol precursors might be bioconverted to equol during fermentation.…”

Section: Introductionmentioning

confidence: 99%

Fermentation product with new equol‐producing Lactobacillus paracasei as a probiotic‐like product candidate for prevention of skin and intestinal disorder

et al. 2019

BACKGROUND: Equol is a major isoflavone metabolite, and equol-producing bacteria have been isolated and characterized; however, fermentation has been performed with soybean-based products as substrates. Pueraria lobata has been reported as a plant with higher content of isoflavones. RESULTS:The genome of new equol-producing bacteria, Lactobacillus paracasei JS1, was analyzed. Also, the effect of P. lobata extract fermented with L. paracasei JS1 (FPE) on the skin and intestinal immune response was examined. With gene expression analysis, it was proven that seven skin-related proteins, hyaluronan synthase-1, -2, -3, collagen, elastin, epidermal growth factor, and epidermal growth factor receptor were differentially expressed upon FPE treatment. The messenger RNA expression increased with treatment with the FPE, and a skin moisturizing effect was confirmed by a hematoxylin-eosin staining experiment. In addition, such an experiment showed that proinflammatory cytokines, tumor necrosis factor-, cyclooxygenase-2, inducible nitric oxide synthase, interleukin-1 , -4, and -6, were reduced in large intestine when treated with FPE. CONCLUSION: L. paracasei JS1 has the ability to produce equol having beneficial effects on the skin. Moreover, FPE also has an inhibitory effect on inflammation cytokines in the large intestine. Thus, the novel and edible equol-producing L. paracasei JS1 and FPE have thepotential to be developed as nutricosmetic resources. Experimental animalsSix-week-old male C57BL/6J mice (RaonBio, Yongin, Korea) were housed in an air-conditioned animal room over a 12-h light/dark cycle at a temperature of 22 ± 1 ∘ C and humidity of 50 ± 10%. The J Sci Food Agric 2019; 99: 4200-4210