Potential role of vitamins in chromium induced spermatogenesis in Swiss mice

Introduction Uptake of Hexavalent chromium (Cr 6) by the testis and its subsequent reduction to trivalent chromium is well known. Several studies attributed Cr 6 testicular toxicity to oxidative stress. Vitamin E is a lipid soluble antioxidant prevents damage to membranes. Selenium (Se) is an essential micronutrient with an antioxidant activity. This study aimed to investigate the potential protective role of vitamin E and selenium against sub-chronic toxicity of Cr 6 on testes of adult male albino rats through biochemical, cytogenetic and sperm analysis. Methodology: The duration of the present study was six weeks and was carried out on 60 adult male albino rats. They were divided into 10 rats of six groups, control group, selenium group (0.5 mg/kg by oral gavage), vitamin E group (125 mg/kg by oral gavage), Cr 6 group (10 mg/kg by oral gavage), Cr 6 + Selenium group and Cr 6 + Vitamin E group. Biochemical, cytogenetic and sperm analysis were done to all groups. Results: Levels of cholesterol, triglyceride, micronucleus polychromatic erythrocyte, total sperm abnormality are increased while glucose, serum glutathione-s-transferase, testosterone, DNA and RNA contents, sperm viability and content decreased. Treatment with Se or vit.E improved all these effects. Conclusion: Hexavalent chromium induced changes in biochemical, cytogenetic and sperm analysis, which lead to disruption in reproductive cell functions. Se or vit. E has the ability for reduction of these deleterious effects. Recommendation: Since vitamin E and selenium have antioxidant properties and play vital role in preventing hexavalent chromium induced toxicity, dietary supplementation with them is recommended for exposed workers and general population.

show abstract

“…Also, Cr induces generation of ROS and disruption of blood-testis barrier with consequent accumulation of Cr in the testis (Acharya et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Vitamin E (vit. E) is a natural component of bilayer membrane lipid and thus helps to maintain membrane stability (Acharya et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The Potential Protective Role of Vitamin E and Selenium against Sub-chronic Toxicity of Hexavalent Chromium on the Testis of Adult Male Albino Rats

Mohammed

Abdelwahab

2020

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

View full text Add to dashboard Cite

show abstract

“…4). The molecular and cellular effects of Vit E have been explained by acting as an antioxidant preventing damage to membranes or proteins due to an antioxidant activity (Acharya et al,2004; Traber et al,2007). Vit E may effectively minimize lipid peroxidation in biological systems (Aldana et al,2001; Giray et al, 2007; Zingg,2007), and it is considered as the first line of defense against lipid peroxidation (Sweetman et al,1997), protecting cell membranes from an early stage of free radical attack.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative and antigenotoxic effect of vitamin E against patulin cytotoxicity and genotoxicity in HepG2 cells

Ayed-Boussema

Abassi

Bouaziz

et al. 2011

Environmental Toxicology

View full text Add to dashboard Cite

Patulin (PAT) is a mycotoxin produced in fruits, mainly in apples, by certain species of Penicillium, Aspergillus, and Byssochlamys. It has been shown that PAT is cytotoxic, genotoxic, and mutagenic in different cell types. Several studies incriminate the oxidative stress as a mechanism of PAT-mediated toxicity. In this context, our aim was to investigate the protective role of Vitamin E (Vit E), an antioxidant agent, against PAT induced cytotoxicity and genotoxicity in cultured HepG2 cells. The obtained results showed that addition of Vit E in cells treated with PAT significantly reduce cell mortality induced by this toxin. In the same conditions, Vit E decreased the intracellular level of ROS, reduced PAT induced p53 expression, and reversed PAT induced DNA damage. In addition, Vit E prevented significantly the percentage of chromosome aberrations induced by PAT in HepG2 cells in a concentration dependant manner. These results suggest that Vit E, an exogenous antioxidant agent, plays an important role in defense against PAT-induced cytotoxicity and genotoxicity, which confirms the involvement of oxidative stress in the induction of DNA damage by PAT in HepG2 cells.

show abstract

“…Boric acid is also a reproductive toxicant which reduces the testosterone level 65 . Chromium compounds mainly cause testicular tissue damage by increasing oxidative stress 66 . Aluminium causes reduced weight of reproductive organs and impair fertility 67 .…”

Section: Hormonalmentioning

confidence: 99%

Untitled

2014

IJPSR

View full text Add to dashboard Cite

Male infertility is a reproductive health disorder, emerging globally as serious medicaland social problem that results in much trauma, emotional instability and psychological stress of the affected couples. In 50% of the diagnosed cases of infertility males are reported to be the predominant causative factor. Male infertility can assess by the quality and quantity of sperm cells as well as structure. The present review provides an overview on a wide range of factors that are accountable for male infertility. Hormonal imbalances, genetical defects, reproductive anatomical & morphological abnormalities, chemicals & toxins exposure, reactive oxygen species and smoking are the main causative factors. Besides these, pollution, changed lifestyle, lack of proper diet & nutrition, addictions, diseases, medications, illness and psychological problems may also contribute to infertility problems which can temporarily or permanently affect male reproductive system. Some of these factors are lifestyle borne and can possibly be corrected and some are medical which need to be given special attention while some fail to be diagnosed. The knowledge of male infertility is increasing rapidly and will enormously help diagnostic and therapeutic approaches to recuperate human infertility.

show abstract

Potential role of vitamins in chromium induced spermatogenesis in Swiss mice

Cited by 38 publications

References 69 publications

The Potential Protective Role of Vitamin E and Selenium against Sub-chronic Toxicity of Hexavalent Chromium on the Testis of Adult Male Albino Rats

The Potential Protective Role of Vitamin E and Selenium against Sub-chronic Toxicity of Hexavalent Chromium on the Testis of Adult Male Albino Rats

Antioxidative and antigenotoxic effect of vitamin E against patulin cytotoxicity and genotoxicity in HepG2 cells

Untitled

Contact Info

Product

Resources

About