Potential Role of Mannose-Binding Lectin in Intrauterine Transmission of Hepatitis B Virus

Wu, Yan; Zhou, Qunyan; Wang, Huihua; Tian, Ting; Zhu, Qingyi; Wang, Hanzhi; Bai, Xuefeng; Yang, Xiaofu; Wang, Zhengping; Dong, Minyue

doi:10.7883/yoken.66.391

Cited by 8 publications

(7 citation statements)

References 10 publications

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“…For example, mannose-binding lectin can combine with HBV directly by identifying the N-acetyl glucosamine and mannose sugar structure on the envelope of HBV, resulting in viral clearance (2,15). In addition, research suggests that the surface antigen entire deglycosylation of virus particles could affect the stability of the protein, change its space structure, and even cause the virion to lose its immunogenicity (10).…”

Section: Discussionmentioning

confidence: 99%

Role of Dendritic Cell–Specific ICAM-3-Grabbing Nonintegrin on Dendritic Cells in the Recognition of Hepatitis B Virus

et al. 2015

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Dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) is an essential process for virus infection, such as HIV and hepatitis C, and plays a role in immune escape. However, the role of DC-SIGN in hepatitis B virus (HBV) infection is still unknown. The aim of this study was to investigate the role of DC-SIGN in mediating the maturation and activation of dendritic cells (DCs) when infected by HBV. Highly mannosylated HBV particles were obtained by treating HBV-producing HepG2.2.15 cells with the a-mannosidase I-inhibitor kifunensine. Highly mannosylated HBV or wild type HBV was added to infect the DCs of the DC-SIGN gene-silencing group and normal group, respectively. Then, the expression of CDla, CD80, CD83, CD86 and HLA-DR on DCs was detected by flow cytometry, the capacity of stimulating lymphocyte proliferation was tested by MTT assay, the level of IL-12p70 that was released by DCs was measured by enzyme-linked immunosorbent assay, and the expression of the proteins NF-κBp65 and p38 was detected by western blot. Both wild type and highly mannosylated HBV could promote DCs maturation and activation. However, the highly mannosylated HBV could promote DCs immune activation more strongly. The difference in the effect on DCs between the two types of HBV could be eliminated by DC-SIGN gene silencing. DC-SIGN can promote the maturation and activation of DCs when recognized HBV, but wild type HBV can escape recognition by DC-SIGN to a certain extent with the help of demannosylated modification, leading to defective DCs function and chronic HBV infection.

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Section: Discussionmentioning

confidence: 99%

Role of Dendritic Cell–Specific ICAM-3-Grabbing Nonintegrin on Dendritic Cells in the Recognition of Hepatitis B Virus

et al. 2015

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“…It has been revealed that MBL polymorphisms that confer low levels of MBL are correlated with poor prognoses such as HBV persistence [Thio et al, ], disease progression [Yuen et al, ; Song le et al, ; Chong et al, ]. It was shown that low fetal MBL is associated with intrauterine infection of HBV [Wu et al, ]. In this study, it was found HBV in vitro up‐regulated MBL expression of trophoblast but the expression of placental MBL did not significantly differ among non‐infected, infected, and control groups.…”

Section: Discussionmentioning

confidence: 59%

Potential roles of placental human beta‐defensin‐3 and apolipoprotein B mRNA‐editing enzyme catalytic polypeptide 3G in prevention of intrauterine transmission of hepatitis B virus

Bai

Tian

Wang

et al. 2014

Journal of Medical Virology

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Approximately 5% of newborns were infected by hepatitis B virus (HBV) via intrauterine transmission and this is the main reason for high prevalence of HBV in endemic regions. However, the mechanisms by which intrauterine transmission is avoided in most cases remain elusive and placental natural anti-microbial factors may play a role in the prevention of HBV intrauterine transmission. The expression levels of human β-defensin-3 (HBD-3), apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G (A3G) and mannose binding lectin (MBL) were determined in the placenta of 30 HBV-seronegative pregnant women (controls), 7 HBV-seropositive pregnant women with infants infected via intrauterine transmission (infected group) and 30 HBV-seropositive pregnant women with non-infected infants (non-infected group). The expression of HBD-3, A3G, and MBL of placental trophoblast cell line Swan71 was determined after exposed to HBV. There were significant differences in placental HBD-3 and A3G levels among three groups, but the expression of MBL did not significantly differ. The expressions of HBD-3 and A3G were higher in non-infected group than controls and infected group, but not significantly different between infected group and controls. The exposure to HBV increased significantly the expression of HBD-3, A3G, and MBL by Swan 71. It may be concluded HBV up-regulates HBD-3 and A3G expression in vivo and in vitro in placental trophoblast and lack of this up-regulation is possibly associated with intrauterine transmission of HBV.

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“…With a mother to child transmission rate of HBV in the region of 5 -10%, intra-uterine transmission of HBV may be related to a failure of HBV infected mothers to stimulate an increase in foetal MBL levels to combat the virus. It has been shown that non-infected neonates of HBV mothers have an induced increase in MBL levels conferring protection whilst neonates born with HBV have a significantly lower level of MBL [37].…”

Section: Mbl and Hepatitis B Virus (Hbv)mentioning

confidence: 99%

Association of Mannose-Binding Lectin Gene Polymorphisms with Liver Diseases: A Review

Lo¹,

Austin²,

Freeman³

2018

MEDJ

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Mannose-Binding Lectin (MBL) is a member of the collectin family and is an important protein in the immune system. It is a pathogen pattern-recognition molecule that binds to specific carbohydrate motifs on the surface of many pathogens. MBL activates complement via lectin pathway. Single nucleotide polymorphisms in the MBL gene influence serum MBL concentration and function. MBL deficiencies increase the risk of infection and disease-specific complications, especially in those who are already immune compromised with pre-existing conditions. This review discusses the molecular genetics of human MBL and the association of MBL polymorphisms with liver diseases including liver fibrosis, viral hepatitis B, viral hepatitis C, and infection post-liver transplantation.

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Potential Role of Mannose-Binding Lectin in Intrauterine Transmission of Hepatitis B Virus

Cited by 8 publications

References 10 publications

Role of Dendritic Cell–Specific ICAM-3-Grabbing Nonintegrin on Dendritic Cells in the Recognition of Hepatitis B Virus

Role of Dendritic Cell–Specific ICAM-3-Grabbing Nonintegrin on Dendritic Cells in the Recognition of Hepatitis B Virus

Potential roles of placental human beta‐defensin‐3 and apolipoprotein B mRNA‐editing enzyme catalytic polypeptide 3G in prevention of intrauterine transmission of hepatitis B virus

Association of Mannose-Binding Lectin Gene Polymorphisms with Liver Diseases: A Review

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