Potential Protection of Curcumin against Hypoxia-induced Decreases in Beta-III Tubulin Content in Rat Prefrontal Cortical Neurons

Abstract

The central nervous system (CNS) is highly dependent on adequate supply of oxygen and is sensitive to hypoxia. It is known that hypoxia induces injuries on the brain tissue and the neuronal activity. Curcumin, a yellow pigment obtained from the rhizome of C. longa Linn., has been regarded as a multi-functional drug with antioxidative activity. In the present study, we first demonstrated a significant decrease in the content of beta-III tubulin protein in rat prefrontal cortex (PFC) tissues induced by repeated hypoxia, but not in rat cerebellum tissue. These suggest a relatively higher sensitivity and probably a higher vulnerability of rat PFC tissue to hypoxia in vivo. We reconfirmed the effect of hypoxia to primary cultured neurons from rat PFC and found a significant decrease in the contents of beta-III tubulin protein after chronic exposure to hypoxia. Moreover, we demonstrated that the hypoxia-induced decrease in beta-III tubulin protein content could be restored by curcumin, suggesting a potential protection of curcumin against hypoxia-induced decreases in beta-III tubulin content in rat PFC neurons.

High TUBB3 Expression, an Independent Prognostic Marker in Patients with Early Non–Small Cell Lung Cancer Treated by Preoperative Chemotherapy, Is Regulated by K-Ras Signaling Pathway

“…Indeed, in ovarian cancer cells, but not in HeLa cervix cancer cells, hypoxia was reported to induce TUBB3 expression (15). Conversely, we repeatedly detected a decrease of TUBB3 protein content in bronchial cell lines cultured in hypoxia chamber as previously reported in some specific subset of neurons (48). Collectively with the data from the LACE-bio study in which all tumor specimens were chemo-naive (34), these findings would point out that a chemotherapy influence on TUBB3 synthesis cannot explain the TUBB3 prognostic value, and the lack of predictive impact (paclitaxel vs. gemcitabine) in our study population.…”

Central nervous system niche involvement in the leukemia

“…The perivascular network regulates neuroblast migration both through contact-dependent and humoral signals. Master components of the extra-cellular matrix such as tenascin, laminin, heparin and chondroitin sulfate proteoglycans play a pivotal role in addressing the appropriate routes to neuronal migration; as a counterpart, transient amplifying progenitors from NSCs have been shown to express a6b1 integrin that mediates parenchymal migration [48,49].…”

Curcumin-decorated nanoliposomes with very high affinity for amyloid-β1-42 peptide

“…It has been shown to be a potent anti-oxidant and anti-inflammatory compound with a favorable toxicity profile [25]; and a free (nitric oxideebased) radical scavenger [26], which protects the brain from lipid peroxidation [27]. Curcumin inhibits amyloid Ab1-42 oligomer formation and cell toxicity at micromolar concentrations in vitro, [28e32] and binds to plaques reducing amyloid levels in vivo [30].…”