Potential of stem cell seeded three-dimensional scaffold for regeneration of full-thickness skin wounds

Abstract

Hypoxic wounds are tough to heal and are associated with chronicity, causing major healthcare burden. Available treatment options offer only limited success for accelerated and scarless healing. Traditional skin substitutes are widely used to improve wound healing, however, they lack proper vascularization. Mesenchymal stem cells (MSCs) offer improved wound healing; however, their poor retention, survival and adherence at the wound site negatively affect their therapeutic potential. The aim of this study is to enhance skin regeneration in a rat model of full-thickness dermal wound by transplanting genetically modified MSCs seeded on a three-dimensional collagen scaffold. Rat bone marrow MSCs were efficiently incorporated in the acellular collagen scaffold. Skin tissues with transplanted subcutaneous scaffolds were histologically analysed, while angiogenesis was assessed both at gene and protein levels. Our findings demonstrated that three-dimensional collagen scaffolds play a potential role in the survival and adherence of stem cells at the wound site, while modification of MSCs with jagged one gene provides a conducive environment for wound regeneration with improved proliferation, reduced inflammation and enhanced vasculogenesis. The results of this study represent an advanced targeted approach having the potential to be translated in clinical settings for targeted personalized therapy.

3D bio scaffold support osteogenic differentiation of mesenchymal stem cells

“…Although the use of MSCs is a common cell source for tissue engineering applications (Aslam et al, 2020; Ekram et al, 2021; Khalid et al, 2022; Sahibdad et al, 2023; Zhao et al, 2018), these cells can self‐renew, survive, and differentiation is highly dependent on the ECM that surrounds them (Khan et al, 2022; Sundaram et al, 2018). Human UC‐derived MSCs provide many advantages over other stem cell sources, including easier access, improved osteochondral differentiation in comparison to other stem cell sources, fewer ethical concerns, and proliferative potency (Basiri et al, 2019; Vautrin‐Glabik et al, 2020).…”

The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds

“…Wang et al, used collagen-binding peptides to induce the homing of MSCs at the wound site [ 35 ]. Some researchers also showed that nanofiber scaffolds could be used to immobilize MSCs with promising results [ 36 , 37 , 38 , 39 ]. As scaffolds from acellular matrices have attracted much attention in the treatment of degenerative diseases, their use has been proposed to improve wound healing.…”

Extracellular Matrix Based 3D Scaffold for Directing the Fate of Adult Stem Cells into Osteogenic Lineage

“…For tissue engineering applications, mesenchymal stem cells (MSCs) are a prevalent cell source [5][6][7]. Selfrenewal, survival, and differentiation potential of these cells are all heavily dependent on the extracellular matrix (ECM) that surrounds them [8,9]. As a result, one of the primary problems is to develop a biomaterial scaffold that can accurately imitate the microenvironment of the native tissue.…”