The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds

Abstract

The treatment of full-thickness skin wounds is a problem in the clinical setting, as they do not heal spontaneously. Extensive pain at the donor site and a lack of skin grafts limit autogenic and allogeneic skin graft availability. We evaluated fetal bovine acellular dermal matrix (FADM) in combination with human Wharton’s jelly mesenchymal stem cells (hWJ-MSCs) to heal full-thickness skin wounds. FADM was prepared from a 6-month-old trauma-aborted fetus. WJ-MSCs were derived from a human umbilical cord and seeded on the FADM. Rat models of full-thickness wounds were created and divided into three groups: control (no treatment), FADM, and FADM-WJMSCs groups. Wound treatment was evaluated microscopically and histologically on days 7, 14, and 21 post-surgery. The prepared FADM was porous and decellularized with a normal range of residual DNA. WJ-MSCs were seeded and proliferated on FADM effectively. The highest wound closure rate was observed in the FADM-WJMSC group on days 7 and 14 post-surgery. Furthermore, this group had fewer inflammatory cells than other groups. Finally, in this study, we observed that, without using the differential cell culture media of fibroblasts, the xenogeneic hWJSCs in combination with FADM could promote an increased rate of full-thickness skin wound closure with less inflammation.

Applications of blood plasma derivatives for cutaneous wound healing: A mini-review of clinical studies

“…With advanced therapies like growth factors, extracellular matrices, synthetic skin, and negative pressure wound therapy; chronic wound care would have become its specialty [ [9] , [10] , [11] ]. Platelets and their derivatives have a considerable role in tissue regeneration.…”

Expression Profile of New Marker Genes Involved in Differentiation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells into Chondrocytes, Osteoblasts, Adipocytes and Neural-like Cells

“…Zhang et al [ 11 ] reported the construction of artificial periosteum with methacrylamide gelatin hydrogel and Wharton’s jelly microparticles for bone regeneration, which resulted in BM-MSCs recruitment and promotion of cell proliferation in rats. Mansour et al [ 12 ] utilized the fetal bovine acellular dermal matrix seeded with WJ-MSCs for healing full-thickness skin wounds in rats. Ohers researchers demonstrated the electrospun poly (ε-caprolactone) and collagen scaffolds seeded with WJ-MSCs for skin tissue regeneration [ 13 ], silk and gelatin nanofibrous 3D scaffolds for differentiation of WJ-MSCs into islet-like cells [ 14 ], and many others.…”