Potential of random amplified microsatellites (RAMS) to typify and discriminate varieties of Physalis ixocarpa Brot. ex Hornem.

Abstract

Physalis ixocarpa is an edible species of Solanaceae. This is one of the few cultivated and economically important species of the genus in Mesoamerica. In Mexico, several varieties and landraces have been developed, which have not been molecularly characterized. In the current study, five RAMS primers were used to characterize and assess the genetic variability of two varieties and three landraces of this species. The capacity of these markers to discriminate between them was also evaluated. With comparative aims, Physalis peruviana, the most economically important species of the genus in South America, was analyzed in the same manner. The results revealed that the varieties and landraces of P. ixocarpa conserve important levels of genetic variability (21.75% > Polymorphism < 42.75%), which were higher than that found for P. peruviana (10.75% Polymorphism). RAMS were useful specific markers, as P. peruviana and P. ixocarpa were clearly distinguished one from each other by both cluster analysis and principal components analysis. Close genetic relationships were found between the landraces San Isidro Chihuiro and Verde Puebla, and between the varieties Diamante and Rendidora. In spite of the genetic closeness, the RAMS amplification profiles had a clear varietal-specific tendency, in such a way that they may represent varietal fingerprints, which can be used as authentication tool for varieties and landraces of P. ixocarpa.

Random amplified microsatellites (RAMS) analysis ascertains genetic variation of Alternaria alternata causing black spot disease on Carya illinoinensis in South Africa

“…The acquired gDNA purity for all the A. alternata isolates was ≥1.7 (A260 nm/A280 nm) ( Sambrook et al, 2006 ), with the concentrations ranging between 10.0 and 802.8 ng/μL. Samples were diluted in sterile water to a concentration of 10 ng/μL, which was sufficient for RAMs molecular analysis ( Delgado-Alvarado et al, 2018 ).…”

“…Random amplified microsatellites (RAMs) are based on Polymerase Chain Reaction (PCR) and is effective to detect high polymorphism levels for genetic diversity studies ( Delgado-Alvarado et al, 2018 ). RAMs markers are co-dominant, fast, reproducible, and are also expressed throughout the fungal genome ( Hantula et al, 1996 ; Hantula and Müller, 1997 ).…”

Advances in Physalis molecular research: applications in authentication, genetic diversity, phylogenetics, functional genes, and omics

“…genotypes, with a polymorphism rate of 22%. Delgado-Alvarado et al. (2018) applied RAMS markers to authenticate the varieties and landraces of Physalis ixocarpa Brot.…”