Potential of Microscopic Fungi Isolated from Mercury Contaminated Soils to Accumulate and Volatilize Mercury(II)

Urík, Martin; Hlodák, Michal; Mikušová, Petra; Matúš, Peter

doi:10.1007/s11270-014-2219-z

Cited by 38 publications

(19 citation statements)

References 39 publications

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“…Their bioremediation potential has been recently explored, i.e., HMs removal capacity from single/multi-metal solutions or contaminated soil bioaugmented with fungal consortia, and their ability to assist in phytoremediation of HM-contaminated soil [ 24 , 31 , 32 , 43 , 44 , 86 , 87 , 88 ]. Unique fungal diversity was identified in various HM-contaminated sites [ 24 , 31 , 43 , 44 , 87 , 88 ]. Similar investigations on bacterial populations reflect the same results [ 56 , 89 , 90 , 91 ].…”

Section: Discussionmentioning

confidence: 99%

“…The preponderance of Fusarium and of other genera identified here (i.e., Penicillium , Lecanicillium, and Phoma ) has also been reported in contaminated areas or substrates containing Cu, Pb, or Zn [ 71 , 95 , 102 ]. Concerning fungal diversity from a former Hg mining plant in Rudňany, Slovakia, Cladosporium cladosporioides , Penicillium spp., Aspergillus spp., and Fusarium oxysporum species were recovered [ 88 ]. However, the composition of fungal communities is site-specific, influenced by soil composition, properties, and selective pressure of HMs [ 37 , 71 , 95 , 99 ].…”

Section: Discussionmentioning

confidence: 99%

“…This facilitates supplementary intracellular uptake, unlike powdered biosorbents. Living fungal cells recovered from contaminated substrates have biosorption capacities in Hg-contaminated medium, in the 0.017–40.2 mg/g Hg 2+ range [ 80 , 88 , 112 , 118 ]. Finally, the Hg 2+ bioremediation potential from highly contaminated aqueous substrate was demonstrated for five species which were isolated and identified in this study.…”

Section: Discussionmentioning

confidence: 99%

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Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

Văcar

Covaci

Chakraborty

et al. 2021

JoF

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Filamentous fungi native to heavy metals (HMs) contaminated sites have great potential for bioremediation, yet are still often underexploited. This research aimed to assess the HMs resistance and Hg remediation capacity of fungi isolated from the rhizosphere of plants resident on highly Hg-contaminated substrate. Analysis of Hg, Pb, Cu, Zn, and Cd concentrations by X-ray spectrometry generated the ecological risk of the rhizosphere soil. A total of 32 HM-resistant fungal isolates were molecularly identified. Their resistance spectrum for the investigated elements was characterized by tolerance indices (TIs) and minimum inhibitory concentrations (MICs). Clustering analysis of TIs was coupled with isolates’ phylogeny to evaluate HMs resistance patterns. The bioremediation potential of five isolates’ live biomasses, in 100 mg/L Hg2+ aqueous solution over 48 h at 120 r/min, was quantified by atomic absorption spectrometry. New species or genera that were previously unrelated to Hg-contaminated substrates were identified. Ascomycota representatives were common, diverse, and exhibited varied HMs resistance spectra, especially towards the elements with ecological risk, in contrast to Mucoromycota-recovered isolates. HMs resistance patterns were similar within phylogenetically related clades, although isolate specific resistance occurred. Cladosporium sp., Didymella glomerata, Fusarium oxysporum, Phoma costaricensis, and Sarocladium kiliense isolates displayed very high MIC (mg/L) for Hg (140–200), in addition to Pb (1568), Cu (381), Zn (2092–2353), or Cd (337). The Hg biosorption capacity of these highly Hg-resistant species ranged from 33.8 to 54.9 mg/g dry weight, with a removal capacity from 47% to 97%. Thus, the fungi identified herein showed great potential as bioremediators for highly Hg-contaminated aqueous substrates.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

Văcar

Covaci

Chakraborty

et al. 2021

JoF

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“…Microscopic filamentous fungus Aspergillus niger (Tiegh. ), strain CBS 140837, originally isolated from the mercury-contaminated soil [ 33 ], was grown in Sabouraud growth medium (HiMedia, Mumbai, India) in 250 mL Erlenmeyer flasks using a 7-day static cultivation in a growth chamber (dark, 25 °C). Four different types of growth media were created with three forms of Zn at 6.5 mg Zn∙L −1 (0.1 mmol Zn∙L −1 ), ZnSO 4 , ZnO NP, and bulk ZnO, and one control without added Zn.…”

Section: Methodsmentioning

confidence: 99%

Fungus Aspergillus niger Processes Exogenous Zinc Nanoparticles into a Biogenic Oxalate Mineral

Šebesta

Urík

Bujdoš

et al. 2020

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Zinc oxide nanoparticles (ZnO NPs) belong to the most widely used nanoparticles in both commercial products and industrial applications. Hence, they are frequently released into the environment. Soil fungi can affect the mobilization of zinc from ZnO NPs in soils, and thus they can heavily influence the mobility and bioavailability of zinc there. Therefore, ubiquitous soil fungus Aspergillus niger was selected as a test organism to evaluate the fungal interaction with ZnO NPs. As anticipated, the A. niger strain significantly affected the stability of particulate forms of ZnO due to the acidification of its environment. The influence of ZnO NPs on fungus was compared to the aqueous Zn cations and to bulk ZnO as well. Bulk ZnO had the least effect on fungal growth, while the response of A. niger to ZnO NPs was comparable with ionic zinc. Our results have shown that soil fungus can efficiently bioaccumulate Zn that was bioextracted from ZnO. Furthermore, it influences Zn bioavailability to plants by ZnO NPs transformation to stable biogenic minerals. Hence, a newly formed biogenic mineral phase of zinc oxalate was identified after the experiment with A. niger strain’s extracellular metabolites highlighting the fungal significance in zinc biogeochemistry.

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“…The fungi Aspergillus niger and Cladosporium isolates volatilized almost 80% of initial mercury content during 7‐day static cultivation in the darkness (Urik et al. ). Fischer et al.…”

Section: Introductionmentioning

confidence: 99%

A new mercury‐accumulating Mucor hiemalis strain EH8 from cold sulfidic spring water biofilms

Hoque

Fritscher

2016

MicrobiologyOpen

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Here, we report about a unique aquatic fungus Mucor hiemalis EH8 that can remove toxic ionic mercury from water by intracellular accumulation and reduction into elemental mercury (Hg0). EH8 was isolated from a microbial biofilm grown in sulfidic‐reducing spring water sourced at a Marching's site located downhill from hop cultivation areas with a history of mercury use. A thorough biodiversity survey and mercury‐removal function analyses were undertaken in an area of about 200 km2 in Bavaria (Germany) to find the key biofilm and microbe for mercury removal. After a systematic search using metal removal assays we identified Marching spring's biofilm out of 18 different sulfidic springs' biofilms as the only one that was capable of removing ionic Hg from water. EH8 was selected, due to its molecular biological identification as the key microorganism of this biofilm with the capability of mercury removal, and cultivated as a pure culture on solid and in liquid media to produce germinating sporangiospores. They removed 99% of mercury from water within 10–48 h after initial exposure to Hg(II). Scanning electron microscopy demonstrated occurrence of intracellular mercury in germinating sporangiospores exposed to mercury. Not only associated with intracellular components, but mercury was also found to be released and deposited as metallic‐shiny nanospheres. Electron‐dispersive x‐ray analysis of such a nanosphere confirmed presence of mercury by the HgMα peak at 2.195 keV. Thus, a first aquatic eukaryotic microbe has been found that is able to grow even at low temperature under sulfur‐reducing conditions with promising performance in mercury removal to safeguard our environment from mercury pollution.

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Potential of Microscopic Fungi Isolated from Mercury Contaminated Soils to Accumulate and Volatilize Mercury(II)

Cited by 38 publications

References 39 publications

Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

Fungus Aspergillus niger Processes Exogenous Zinc Nanoparticles into a Biogenic Oxalate Mineral

A new mercury‐accumulating Mucor hiemalis strain EH8 from cold sulfidic spring water biofilms

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