Potential of <em>Annona muricata</em> L. seed oil: phytochemical and nutritional characterization associated with non-toxicity

Abstract

SUMMARY:The aim of this study was to evaluate the nutritional quality, phenolic compounds, fatty acid and antioxidant activity in vitro as well as a toxicological screening of A. muricata seed oil in vivo. The chemical composition and quantification of phenolic compounds were determined by the Adolfo Lutz Institute normative. The antioxidant activity was evaluated by DPPH, FRAP and ABTS methods. The oil was extracted by chloroform/ methanol and precipitated crude (AmPtO) and supernatant oils (AmSO) were obtained. The fatty acid profile was evaluated by gas chromatography and total compounds by HPLC-DAD. BALB/C mice received AmPtO and AmSO (0.5 and 1.0mL·Kg -1 ) for 14 days. Toxicity parameters were assessed. The major fatty acids in the oil were oleic (39.2%) and linoleic (33%). HPLC-DAD suggested the presence of acetogenins (annonacin: 595 [M-H] -), with a greater presence in AmPtO. The AmPtO group showed toxicity, which may be related to the acetogenin content in AmPtO. The AmSO group showed no toxicity and this oil has potential for food or medicinal use. KEYWORDS:Acetogenins; Annona muricata seeds; Annonacin; Linoleic acid; Oleic acid RESUMEN: Potencial del aceite de Annona muricata L.: Caracterización nutricional y fitoquímica asociada con no toxicidad. El objetivo de este estudio fue evaluar la calidad nutricional, compuestos fenólicos, ácidos grasos, actividad antioxidante in vitro y evaluación toxicológica del aceite de las semillas de la Annona muricata. La composición química y los compuestos fenólicos se determinaron de acuerdo con las normas del Instituto Adolfo Lutz y la actividad antioxidante por métodos de DPPH, FRAP y ABTS. El aceite fue extraído con cloroformo/metanol obteniéndose un precipitado crudo (AmPtO) y un aceite sobrenadante (AmSO). El perfil de ácidos grasos fue evaluado por cromatografía gaseosa y los compuestos fitoquímicos mediante HPLC-DAD. BALB/C recibieron AmPtO y AmSO (0,5 y 1,0 mL·kg

Intensified green-based extraction process as a circular economy approach to recover bioactive compounds from soursop seeds (Annona muricata L.)

“…Soursop seed oil extracted by SFE contained 27.6% saturated fatty acids and 70.0% unsaturated fatty acids ( Table 2 ). Similar fatty acid profiles have also been reported in the literature, with unsaturated fatty acid percentages of 64–75% ( Dorado et al, 2016 , Pinto et al, 2018 ). The major compounds identified in terms of the relative area percentage and/or relevance in the extracts were the unsaturated fatty acids, oleic acid (ω9), 10,13-octadecadienoic acid (PUFA), linoleic acid (ω6), palmitoleic acid (ω7), and elaidic acid (a trans geometric isomer of oleic acid), as well as saturated fatty acids, palmitic acid, and stearic acid.…”

“…The SFE extracts afforded high EC 50 values or low AA capacities (ARP values) in the DPPH/ARP assay ( Table 1 ). Pinto et al (2018) also reported that the Annona muricata seed oil showed a low AA in vitro . These results could be explained by the nonpolar characteristics of carbon dioxide that did not favor the solubilization of phenolic compounds of intermediate to high polarities ( Mazzutti et al, 2018 ).…”

“…The results obtained using the SWE method were superior to those reported in the literature for the AAs of the soursop seeds. For example, Pinto et al (2018) obtained extracts using methanol and chloroform as solvents with a value of 40.2 μmol TE∙g −1 of dry extract for the ABTS assay. In a cold extraction study using chloroform, methanol, and water in a 2:1:0.8 (v/v/v) ratio, da Silva and Jorge (2014) reported a value of 0.8 μmol TE∙g −1 of dry extract for the ABTS assay.…”

“…Only a few efforts have been directed toward the industrial exploitation of soursop seeds commonly discarded during the processing of commercially harvested fruit juices and pulps ( Pinto et al, 2018 ). Thus, based on the composition of fatty acids, soursop seeds contain essential compounds that can be applied in food industry.…”

Tópicos em Agroecologia - Volume 2

“…Estudos promissores vem sendo realizados na medicina com compostos isolados das sementes de Annona muricata L. Dentre estes estudos, pode-se citar o potencial de citoxicidade seletiva contra linhas celulares tumorais humanas (ARIFIANTI et al, 2014;RAYBAUDI-MASSILIA et al, 2015) e a melhora nos parâmetros de diabetes tipo 1 in vivo e in vitro (PINTO et al, 2018a).…”