Potential of lignocellulolytic biocatalysts of native and proposed genetically engineered microbial cell factories on jute fiber modification and jute waste recycling: A review

Abstract

The lignocellulolytic microbial systems from different parts of the world responsible for lignocellulosic biomass (LCB) like jute (Corchorus spp.) waste degradation, fiber modification, and bioenergy production are not limited to a specific prokaryotic or eukaryotic group. The industrial applications of these highly efficient bacterial, fungal and algal communities are related to the production of lignocellulolytic enzymes such as cellulase, hemicellulase, lignin-peroxidase, versatile peroxidase, laccase, thermostable oxidants, pectinase, etc. They are a blessing for the jute, dye, paper, pulp, and biofuel industries as they help to generate a sustainable ecosystem. The jute plant is lignocellulosic biomass so it can be utilized in various ways, from everyday goods to power generation. Jute industries generally use different physicochemical strategies to generate quality fiber and post-retting activities, but these approaches cannot produce desired products; hence microbial routes are best for quality fiber generation, waste remediation, and biofuel generation. To this end, this review summarizes the most important milestones of the development of the leading enzyme-producing cell factories and their engineering by genetic, metabolic, and synthetic biology approaches with the emergence of high throughput methods, such as site-directed mutagenesis and others that can analyze the relevant mutations to accelerate our understanding of lignocellulolytic enzymology.

Isolation and Screening of Dye Degrading Lignocellulolytic Bacteria from Sundarban Mangrove Ecosystem, West Bengal, India

“…A total of 5 sequences for laccase, 7 for cellulase, 7 for pectinase from all bacterial species, and 3 sequences from fungal species (supplementary dataset1) were selected for the current study depending on their laccase, cellulase, pectinase activity, ABTS-oxidation capacity for laccase and DNS assay capability (released reducing sugar) for cellulase and pectinase that showed similar enzymatic characteristics with the isolated strains 56,57 (Table S1). After multiple sequence alignments by the MUSCLE algorithm, boot-strapping was done 1000 times in MEGA 11 software.…”