Potential of FTIR spectroscopy in chemical characterization of Termitomyces Pellets

doi:10.7324/jabb.2017.50412

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…FTIR spectroscopy was carried out (Bhat 2013;D'Souza and Kamat 2017) to determine functional groups present in the extract. FTIR spectrometer was used to record the infrared transmittance at a wavenumber ranging from 4000-400 cm -1 of the purified compounds (FTIR, I05 Nicolet Avatar 370, Thermo Scientific, USA).…”

Section: Spectroscopic Methods Of Compound Identification Fourier Tra...mentioning

confidence: 99%

Anticancer activity of secondary metabolite isolated from the rhizospheric fungus Fusarium oxysporum isolate-ABRF1, 2-propenoic acid, pentadecyl ester

SAHU,

SUTHAKARAN,

GHOSH

et al. 2024

Asian J Nat Prod Biochem

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Abstract. Sahu MK, Suthakaran S, Ghosh SC, Singh D, Das A, Jha H. 2023. Anticancer activity of secondary metabolite isolated from the rhizospheric fungus Fusarium oxysporum isolate-ABRF1, 2-propenoic acid, pentadecyl ester. Asian J Nat Prod Biochem 21: 88-100. The rhizospheric fungus Fusarium oxysporum ABRF1 from the Achanakmar Biosphere Reserve, Chhattisgarh, India, was evaluated for its anticancer potency using various cellular and molecular assays. A differential cytotoxic profile of the different fractions of the ABRF1 isolate was observed against various cancer cell lines. DNA fragmentation analysis revealed the aqueous and toluene fractions were effective and correlated well with apoptotic gene expression in several breast cancer cells. The fractions showed markedly increased expression of pro-apoptotic protein markers – BAX and cleaved caspase 3 in the breast cancer cells. Structural and functional characterization of potential secondary metabolite from the fungal isolate ABRF1 was carried out using Gas Chromatography with Mass Spectroscopy and Nuclear Magnetic Resonance that characterized as 2-propenoic acid, pentadecyl ester. The underlying mechanisms responsible for the anticancer property were confirmed by molecular docking analysis of 2-propenoic acid, pentadecyl ester, and positive controls such as Doxorubicin and Noscapine. The results showed an efficient binding with molecular targets such as protein kinases (EphA2) and epithelial-to-mesenchymal transition markers (Vimentin) in breast cancer cells. The active metabolite 2-propenoic acid, pentadecyl ester, has a potential anticancer property that needs to be taken further for in vivo studies and drug development in the future.

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Section: Spectroscopic Methods Of Compound Identification Fourier Tra...mentioning

confidence: 99%

Anticancer activity of secondary metabolite isolated from the rhizospheric fungus Fusarium oxysporum isolate-ABRF1, 2-propenoic acid, pentadecyl ester

SAHU,

SUTHAKARAN,

GHOSH

et al. 2024

Asian J Nat Prod Biochem

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Mushroom’s Evaluation Based on FT-IR Fingerprint and Chemometrics

Feher¹,

Floare-Avram²,

Covaciu³

et al. 2021

Preprint

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Edible mushrooms have been recognized as highly nutritional food for a long time, due to their specific flavor, texture and also for therapeutic effects. This study proposes a new simple approach, based on FT-IR analysis, followed by statistical methods, in order to differentiate three wild mushrooms species from Romanian spontaneous flora, namely Armillaria mellea, Boletus edulis and Cantharellus cibarius. The preliminary data treatment consisted of data set reduction with principal component analysis (PCA), which provided scores for the next methods. Linear discriminant analysis (LDA) manage to 100% classify the three species and the cross validation step of the method returned 97.4% of correctly classified samples. Only one A. mellea sample overlapped on B. edulis group. When kNN was used in the same manner as LDA, the overall percent of correctly classified samples from the training step was 86.21%, while for holdout set the percent raised at 94.74%. The lowered values obtained for the training set was due to one C. cibarius sample, two B. edulis and five A. mellea, which were placed to other species. Anyway, for holdout sample set, only one sample from B. edulis was misclassified. The fuzzy c-means clustering (FCM) analysis successfully classified investigated mushroom samples according to their species, meaning that in every partition the predominant specie had the biggest DOMs, while samples belonging to other specie had lower DOMs.

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Potential of FTIR spectroscopy in chemical characterization of Termitomyces Pellets

Cited by 2 publications

References 41 publications

Anticancer activity of secondary metabolite isolated from the rhizospheric fungus Fusarium oxysporum isolate-ABRF1, 2-propenoic acid, pentadecyl ester

Anticancer activity of secondary metabolite isolated from the rhizospheric fungus Fusarium oxysporum isolate-ABRF1, 2-propenoic acid, pentadecyl ester

Mushroom’s Evaluation Based on FT-IR Fingerprint and Chemometrics

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